A manual method to obtain platelet rich plasma

Detalhes bibliográficos
Autor(a) principal: Marques, Fabiana Paulino
Data de Publicação: 2014
Outros Autores: Ingham, Sheila Jean McNeill [UNIFESP], Forgas, Andrea, Franciozi, Carlos Eduardo da Silveira [UNIFESP], Sasaki, Pedro Henrique, Abdalla, Rene Jorge [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/1413-78522014220200798
http://repositorio.unifesp.br/handle/11600/8166
Resumo: OBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.
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spelling A manual method to obtain platelet rich plasmaPlatelet-rich plasmaBlood plateletsMethods.OBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.Hospital do Coração Knee InstituteUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Department of Orthopedics and TraumatologyUNIFESP, EPM, Department of Orthopedics and TraumatologySciELOSociedade Brasileira de Ortopedia e TraumatologiaHospital do Coração Knee InstituteUniversidade Federal de São Paulo (UNIFESP)Marques, Fabiana PaulinoIngham, Sheila Jean McNeill [UNIFESP]Forgas, AndreaFranciozi, Carlos Eduardo da Silveira [UNIFESP]Sasaki, Pedro HenriqueAbdalla, Rene Jorge [UNIFESP]2015-06-14T13:46:51Z2015-06-14T13:46:51Z2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion75-77application/pdfhttp://dx.doi.org/10.1590/1413-78522014220200798Acta Ortopédica Brasileira. Sociedade Brasileira de Ortopedia e Traumatologia, v. 22, n. 2, p. 75-77, 2014.10.1590/1413-78522014220200798S1413-78522014000200075.pdf1413-7852S1413-78522014000200075http://repositorio.unifesp.br/handle/11600/8166engActa Ortopédica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-06T04:55:53Zoai:repositorio.unifesp.br/:11600/8166Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-06T04:55:53Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv A manual method to obtain platelet rich plasma
title A manual method to obtain platelet rich plasma
spellingShingle A manual method to obtain platelet rich plasma
Marques, Fabiana Paulino
Platelet-rich plasma
Blood platelets
Methods.
title_short A manual method to obtain platelet rich plasma
title_full A manual method to obtain platelet rich plasma
title_fullStr A manual method to obtain platelet rich plasma
title_full_unstemmed A manual method to obtain platelet rich plasma
title_sort A manual method to obtain platelet rich plasma
author Marques, Fabiana Paulino
author_facet Marques, Fabiana Paulino
Ingham, Sheila Jean McNeill [UNIFESP]
Forgas, Andrea
Franciozi, Carlos Eduardo da Silveira [UNIFESP]
Sasaki, Pedro Henrique
Abdalla, Rene Jorge [UNIFESP]
author_role author
author2 Ingham, Sheila Jean McNeill [UNIFESP]
Forgas, Andrea
Franciozi, Carlos Eduardo da Silveira [UNIFESP]
Sasaki, Pedro Henrique
Abdalla, Rene Jorge [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Hospital do Coração Knee Institute
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Marques, Fabiana Paulino
Ingham, Sheila Jean McNeill [UNIFESP]
Forgas, Andrea
Franciozi, Carlos Eduardo da Silveira [UNIFESP]
Sasaki, Pedro Henrique
Abdalla, Rene Jorge [UNIFESP]
dc.subject.por.fl_str_mv Platelet-rich plasma
Blood platelets
Methods.
topic Platelet-rich plasma
Blood platelets
Methods.
description OBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2015-06-14T13:46:51Z
2015-06-14T13:46:51Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1413-78522014220200798
Acta Ortopédica Brasileira. Sociedade Brasileira de Ortopedia e Traumatologia, v. 22, n. 2, p. 75-77, 2014.
10.1590/1413-78522014220200798
S1413-78522014000200075.pdf
1413-7852
S1413-78522014000200075
http://repositorio.unifesp.br/handle/11600/8166
url http://dx.doi.org/10.1590/1413-78522014220200798
http://repositorio.unifesp.br/handle/11600/8166
identifier_str_mv Acta Ortopédica Brasileira. Sociedade Brasileira de Ortopedia e Traumatologia, v. 22, n. 2, p. 75-77, 2014.
10.1590/1413-78522014220200798
S1413-78522014000200075.pdf
1413-7852
S1413-78522014000200075
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Ortopédica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 75-77
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268295201685504

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