Plasma cytokine expression after lower-limb compression in rats

• Autor(a) principal: Sgarbi,Mauricio Wanderley Moral
• Data de Publicação: 2015
• Outros Autores: Silva Júnior,Bomfim Alves, Peres,Carmem Maldonado, Loureiro,Tatiana Carolina Alba, Curi,Rui, Soriano,Francisco Garcia, Ribeiro,Daniel Araki, Velasco,Irineu Tadeu
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Brasileira de Ortopedia (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162015000100105
• topic: Crush syndrome; Animal models; Interleukins; Tumor necrosis factor α

OBJECTIVES: Muscle injury due to crushing (muscle compression injury) is associated with systemic manifestations known as crush syndrome. A systemic inflammatory reaction may also be triggered by isolated muscle injury. The aim of this study was to investigate the plasma levels of interleukins (IL) 1, 6 and 10 and tumor necrosis factor alpha (TNF-α), which are markers for possible systemic inflammatory reactions, after isolated muscle injury resulting from lower-limb compression in rats.METHODS: Male Wistar rats were subjected to 1 h of compression of their lower limbs by means of a rubber band. The plasma levels of IL 1, 6 and 10 and TNF-α were measured 1, 2 and 4 h after the rats were released from compression.RESULTS: The plasma levels of IL 10 decreased in relation to those of the other groups, with a statistically significant difference (p < 0.05). The method used did not detect the presence of IL 1, IL 6 or TNF-α.CONCLUSION: Our results demonstrated that the changes in plasma levels of IL 10 that were found may have been a sign of the presence of circulating interleukins in this model of lower-limb compression in rats.