Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes

Detalhes bibliográficos
Autor(a) principal: Augusto,Lívia Maria Mendonça
Data de Publicação: 2016
Outros Autores: Aguiar,Diego Pinheiro, Bonfim,Danielle Cabral, Cavalcanti,Amanda dos Santos, Casado,Priscila Ladeira, Duarte,Maria Eugênia Leite
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Ortopedia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000100070
Resumo: ABSTRACT OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR. RESULTS: The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression. CONCLUSION: Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.
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spelling Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytesTendonMesenchymal stromal cells from bone marrowTenocytesABSTRACT OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR. RESULTS: The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression. CONCLUSION: Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.Sociedade Brasileira de Ortopedia e Traumatologia2016-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000100070Revista Brasileira de Ortopedia v.51 n.1 2016reponame:Revista Brasileira de Ortopedia (Online)instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)instacron:SBOT10.1016/j.rboe.2015.12.013info:eu-repo/semantics/openAccessAugusto,Lívia Maria MendonçaAguiar,Diego PinheiroBonfim,Danielle CabralCavalcanti,Amanda dos SantosCasado,Priscila LadeiraDuarte,Maria Eugênia Leiteeng2016-03-03T00:00:00Zoai:scielo:S0102-36162016000100070Revistahttp://www.rbo.org.br/https://old.scielo.br/oai/scielo-oai.php||rbo@sbot.org.br1982-43780102-3616opendoar:2016-03-03T00:00Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)false
dc.title.none.fl_str_mv Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
title Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
spellingShingle Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
Augusto,Lívia Maria Mendonça
Tendon
Mesenchymal stromal cells from bone marrow
Tenocytes
title_short Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
title_full Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
title_fullStr Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
title_full_unstemmed Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
title_sort Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
author Augusto,Lívia Maria Mendonça
author_facet Augusto,Lívia Maria Mendonça
Aguiar,Diego Pinheiro
Bonfim,Danielle Cabral
Cavalcanti,Amanda dos Santos
Casado,Priscila Ladeira
Duarte,Maria Eugênia Leite
author_role author
author2 Aguiar,Diego Pinheiro
Bonfim,Danielle Cabral
Cavalcanti,Amanda dos Santos
Casado,Priscila Ladeira
Duarte,Maria Eugênia Leite
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Augusto,Lívia Maria Mendonça
Aguiar,Diego Pinheiro
Bonfim,Danielle Cabral
Cavalcanti,Amanda dos Santos
Casado,Priscila Ladeira
Duarte,Maria Eugênia Leite
dc.subject.por.fl_str_mv Tendon
Mesenchymal stromal cells from bone marrow
Tenocytes
topic Tendon
Mesenchymal stromal cells from bone marrow
Tenocytes
description ABSTRACT OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR. RESULTS: The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression. CONCLUSION: Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000100070
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162016000100070
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.rboe.2015.12.013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
dc.source.none.fl_str_mv Revista Brasileira de Ortopedia v.51 n.1 2016
reponame:Revista Brasileira de Ortopedia (Online)
instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron:SBOT
instname_str Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron_str SBOT
institution SBOT
reponame_str Revista Brasileira de Ortopedia (Online)
collection Revista Brasileira de Ortopedia (Online)
repository.name.fl_str_mv Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
repository.mail.fl_str_mv ||rbo@sbot.org.br
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