Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442016000300142 |
Resumo: | ABSTRACT Introduction: The reticulocyte count by flow cytometry (FC) - an automated counting method - can present errors due to the presence of interfering factors, contributing to a slight increase in results. However, automated methods have large advantages over the manual method, taken as reference, what justifies efforts to improve their quality. Objective: Evaluate platelet interference with the reticulocyte count by FC, using thiazole orange (TO) (FC/TO). Materials and methods: The method of reticulocyte count by FC/TO and a modified automated equivalent method, which excluded CD61-positive cells (platelets) from analysis (FC/TO/MOD), were compared to the manual method. Conclusion: Results were analyzed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI) to assess interchangeability between the methods, by linear regression analysis and paired t-test. The exclusion of interfering fragments from result analysis by the modified method produced results in closer proximity to those of the reference method. |
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Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orangereticulocyte countflow cytometryblood plateletsABSTRACT Introduction: The reticulocyte count by flow cytometry (FC) - an automated counting method - can present errors due to the presence of interfering factors, contributing to a slight increase in results. However, automated methods have large advantages over the manual method, taken as reference, what justifies efforts to improve their quality. Objective: Evaluate platelet interference with the reticulocyte count by FC, using thiazole orange (TO) (FC/TO). Materials and methods: The method of reticulocyte count by FC/TO and a modified automated equivalent method, which excluded CD61-positive cells (platelets) from analysis (FC/TO/MOD), were compared to the manual method. Conclusion: Results were analyzed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI) to assess interchangeability between the methods, by linear regression analysis and paired t-test. The exclusion of interfering fragments from result analysis by the modified method produced results in closer proximity to those of the reference method.Sociedade Brasileira de Patologia Clínica2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442016000300142Jornal Brasileiro de Patologia e Medicina Laboratorial v.52 n.3 2016reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.5935/1676-2444.20160025info:eu-repo/semantics/openAccessViana,Karina AugustaMartins Filho,Olindo A.Dusse,Luci Maria S.Antonelli,Lis R. V.Avelar,Danielle M. V.Avelar,Renato S.Carvalho,Maria das Graçaseng2016-07-28T00:00:00Zoai:scielo:S1676-24442016000300142Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2016-07-28T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange |
title |
Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange |
spellingShingle |
Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange Viana,Karina Augusta reticulocyte count flow cytometry blood platelets |
title_short |
Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange |
title_full |
Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange |
title_fullStr |
Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange |
title_full_unstemmed |
Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange |
title_sort |
Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange |
author |
Viana,Karina Augusta |
author_facet |
Viana,Karina Augusta Martins Filho,Olindo A. Dusse,Luci Maria S. Antonelli,Lis R. V. Avelar,Danielle M. V. Avelar,Renato S. Carvalho,Maria das Graças |
author_role |
author |
author2 |
Martins Filho,Olindo A. Dusse,Luci Maria S. Antonelli,Lis R. V. Avelar,Danielle M. V. Avelar,Renato S. Carvalho,Maria das Graças |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Viana,Karina Augusta Martins Filho,Olindo A. Dusse,Luci Maria S. Antonelli,Lis R. V. Avelar,Danielle M. V. Avelar,Renato S. Carvalho,Maria das Graças |
dc.subject.por.fl_str_mv |
reticulocyte count flow cytometry blood platelets |
topic |
reticulocyte count flow cytometry blood platelets |
description |
ABSTRACT Introduction: The reticulocyte count by flow cytometry (FC) - an automated counting method - can present errors due to the presence of interfering factors, contributing to a slight increase in results. However, automated methods have large advantages over the manual method, taken as reference, what justifies efforts to improve their quality. Objective: Evaluate platelet interference with the reticulocyte count by FC, using thiazole orange (TO) (FC/TO). Materials and methods: The method of reticulocyte count by FC/TO and a modified automated equivalent method, which excluded CD61-positive cells (platelets) from analysis (FC/TO/MOD), were compared to the manual method. Conclusion: Results were analyzed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI) to assess interchangeability between the methods, by linear regression analysis and paired t-test. The exclusion of interfering fragments from result analysis by the modified method produced results in closer proximity to those of the reference method. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442016000300142 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442016000300142 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1676-2444.20160025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.52 n.3 2016 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
_version_ |
1752122296719376384 |