Evaluation of the interference of platelets in reticulocyte counting by flow cytometry using thiazole orange

• Autor(a) principal: Karina Augusta Viana
• Data de Publicação: 2016
• Outros Autores: Olindo Assis Martins Filho, Luci Maria SantAna Dusse, Lis Ribeiro do Valle Antonelli, Danielle Marchetti Vitelli Avelar, Renato Sathler Avelar, Maria das Graças Carvalho
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: https://doi.org/10.5935/1676-2444.20160025; http://hdl.handle.net/1843/59020
• topic: Reticulocyte count; Flow cytometry; Blood platelets; Hematologia; Contagem de reticulócitos; Citometria de fluxo; Plaquetas

Introduction: The reticulocyte count by flow cytometry (FC) – an automated counting method – can present errors due to the presence of interfering factors, contributing to a slight increase in results. However, automated methods have large advantages over the manual method, taken as reference, what justifies efforts to improve their quality. Objective: Evaluate platelet interference with the reticulocyte count by FC, using thiazole orange (TO) (FC/TO). Materials and methods: The method of reticulocyte count by FC/TO and a modified automated equivalent method, which excluded CD61-positive cells (platelets) from analysis (FC/TO/MOD), were compared to the manual method. Conclusion: Results were analyzed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI) to assess interchangeability between the methods, by linear regression analysis and paired t-test. The exclusion of interfering fragments from result analysis by the modified method produced results in closer proximity to those of the reference method.