Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol

Detalhes bibliográficos
Autor(a) principal: Viana,Karina Augusta
Data de Publicação: 2014
Outros Autores: Carvalho,Maria das Graças, Dusse,Luci Maria Sant'Ana, Fernandes,Aline Caldeira, Avelar,Renato Sathler, Avelar,Danielle Marquete Vitelli, Carvalho,Beatriz, Ribeiro,Claudia Maria Franco, Antonelli,Lis Ribeiro do Valle, Teixeira,Andrea, Martins Filho,Olindo Assis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000300189
Resumo: Introduction: Currently, the reticulocyte counting is a challenge for clinical laboratories in Brazil, mainly for the ordinary ones, which still use the manual method. This method has some limitations, since it consists of a laborious method, time consuming, with low accuracy. Objectives: This study has developed and evaluated the performance of a New Laboratory Protocol for flow cytometry (FC) reticulocytes counting using acridine orange (AO) as dye, aiming to standardize a more precise, easy, fast implementation, and low cost protocol. After standardization of the New Protocol (FC/AO), it was compared with the manual method. The results were analyzed according to the recommendations of the National Committee for Clinical Laboratory Standards (NCCLS), now known as Clinical and Laboratory Standards Institute (CLSI), to evaluate the interchangeability of methods in linear regression analysis and paired t test, besides other quality control tests. Conclusion: Based on these results concerning to the correlation between the methods and the tests related to quality control, we can admit that FC/AO for reticulocyte counting shows undeniable advantages when compared to the preexisting manual method.
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spelling Flow cytometry reticulocyte counting using acridine orange: validation of a new protocolreticulocyte counting methodsflow cytometryacridine orangemanual reticulocyte count Introduction: Currently, the reticulocyte counting is a challenge for clinical laboratories in Brazil, mainly for the ordinary ones, which still use the manual method. This method has some limitations, since it consists of a laborious method, time consuming, with low accuracy. Objectives: This study has developed and evaluated the performance of a New Laboratory Protocol for flow cytometry (FC) reticulocytes counting using acridine orange (AO) as dye, aiming to standardize a more precise, easy, fast implementation, and low cost protocol. After standardization of the New Protocol (FC/AO), it was compared with the manual method. The results were analyzed according to the recommendations of the National Committee for Clinical Laboratory Standards (NCCLS), now known as Clinical and Laboratory Standards Institute (CLSI), to evaluate the interchangeability of methods in linear regression analysis and paired t test, besides other quality control tests. Conclusion: Based on these results concerning to the correlation between the methods and the tests related to quality control, we can admit that FC/AO for reticulocyte counting shows undeniable advantages when compared to the preexisting manual method. Sociedade Brasileira de Patologia Clínica2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000300189Jornal Brasileiro de Patologia e Medicina Laboratorial v.50 n.3 2014reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.5935/1676-2444.20140014info:eu-repo/semantics/openAccessViana,Karina AugustaCarvalho,Maria das GraçasDusse,Luci Maria Sant'AnaFernandes,Aline CaldeiraAvelar,Renato SathlerAvelar,Danielle Marquete VitelliCarvalho,BeatrizRibeiro,Claudia Maria FrancoAntonelli,Lis Ribeiro do ValleTeixeira,AndreaMartins Filho,Olindo Assiseng2014-10-02T00:00:00Zoai:scielo:S1676-24442014000300189Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2014-10-02T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false
dc.title.none.fl_str_mv Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
title Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
spellingShingle Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
Viana,Karina Augusta
reticulocyte counting methods
flow cytometry
acridine orange
manual reticulocyte count
title_short Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
title_full Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
title_fullStr Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
title_full_unstemmed Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
title_sort Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
author Viana,Karina Augusta
author_facet Viana,Karina Augusta
Carvalho,Maria das Graças
Dusse,Luci Maria Sant'Ana
Fernandes,Aline Caldeira
Avelar,Renato Sathler
Avelar,Danielle Marquete Vitelli
Carvalho,Beatriz
Ribeiro,Claudia Maria Franco
Antonelli,Lis Ribeiro do Valle
Teixeira,Andrea
Martins Filho,Olindo Assis
author_role author
author2 Carvalho,Maria das Graças
Dusse,Luci Maria Sant'Ana
Fernandes,Aline Caldeira
Avelar,Renato Sathler
Avelar,Danielle Marquete Vitelli
Carvalho,Beatriz
Ribeiro,Claudia Maria Franco
Antonelli,Lis Ribeiro do Valle
Teixeira,Andrea
Martins Filho,Olindo Assis
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Viana,Karina Augusta
Carvalho,Maria das Graças
Dusse,Luci Maria Sant'Ana
Fernandes,Aline Caldeira
Avelar,Renato Sathler
Avelar,Danielle Marquete Vitelli
Carvalho,Beatriz
Ribeiro,Claudia Maria Franco
Antonelli,Lis Ribeiro do Valle
Teixeira,Andrea
Martins Filho,Olindo Assis
dc.subject.por.fl_str_mv reticulocyte counting methods
flow cytometry
acridine orange
manual reticulocyte count
topic reticulocyte counting methods
flow cytometry
acridine orange
manual reticulocyte count
description Introduction: Currently, the reticulocyte counting is a challenge for clinical laboratories in Brazil, mainly for the ordinary ones, which still use the manual method. This method has some limitations, since it consists of a laborious method, time consuming, with low accuracy. Objectives: This study has developed and evaluated the performance of a New Laboratory Protocol for flow cytometry (FC) reticulocytes counting using acridine orange (AO) as dye, aiming to standardize a more precise, easy, fast implementation, and low cost protocol. After standardization of the New Protocol (FC/AO), it was compared with the manual method. The results were analyzed according to the recommendations of the National Committee for Clinical Laboratory Standards (NCCLS), now known as Clinical and Laboratory Standards Institute (CLSI), to evaluate the interchangeability of methods in linear regression analysis and paired t test, besides other quality control tests. Conclusion: Based on these results concerning to the correlation between the methods and the tests related to quality control, we can admit that FC/AO for reticulocyte counting shows undeniable advantages when compared to the preexisting manual method.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/1676-2444.20140014
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Sociedade Brasileira de Patologia Clínica
publisher.none.fl_str_mv
Sociedade Brasileira de Patologia Clínica
dc.source.none.fl_str_mv Jornal Brasileiro de Patologia e Medicina Laboratorial v.50 n.3 2014
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instname:Sociedade Brasileira de Patologia (SBP)
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reponame_str Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)
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