Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000300189 |
Resumo: | Introduction: Currently, the reticulocyte counting is a challenge for clinical laboratories in Brazil, mainly for the ordinary ones, which still use the manual method. This method has some limitations, since it consists of a laborious method, time consuming, with low accuracy. Objectives: This study has developed and evaluated the performance of a New Laboratory Protocol for flow cytometry (FC) reticulocytes counting using acridine orange (AO) as dye, aiming to standardize a more precise, easy, fast implementation, and low cost protocol. After standardization of the New Protocol (FC/AO), it was compared with the manual method. The results were analyzed according to the recommendations of the National Committee for Clinical Laboratory Standards (NCCLS), now known as Clinical and Laboratory Standards Institute (CLSI), to evaluate the interchangeability of methods in linear regression analysis and paired t test, besides other quality control tests. Conclusion: Based on these results concerning to the correlation between the methods and the tests related to quality control, we can admit that FC/AO for reticulocyte counting shows undeniable advantages when compared to the preexisting manual method. |
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Flow cytometry reticulocyte counting using acridine orange: validation of a new protocolreticulocyte counting methodsflow cytometryacridine orangemanual reticulocyte count Introduction: Currently, the reticulocyte counting is a challenge for clinical laboratories in Brazil, mainly for the ordinary ones, which still use the manual method. This method has some limitations, since it consists of a laborious method, time consuming, with low accuracy. Objectives: This study has developed and evaluated the performance of a New Laboratory Protocol for flow cytometry (FC) reticulocytes counting using acridine orange (AO) as dye, aiming to standardize a more precise, easy, fast implementation, and low cost protocol. After standardization of the New Protocol (FC/AO), it was compared with the manual method. The results were analyzed according to the recommendations of the National Committee for Clinical Laboratory Standards (NCCLS), now known as Clinical and Laboratory Standards Institute (CLSI), to evaluate the interchangeability of methods in linear regression analysis and paired t test, besides other quality control tests. Conclusion: Based on these results concerning to the correlation between the methods and the tests related to quality control, we can admit that FC/AO for reticulocyte counting shows undeniable advantages when compared to the preexisting manual method. Sociedade Brasileira de Patologia Clínica2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000300189Jornal Brasileiro de Patologia e Medicina Laboratorial v.50 n.3 2014reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online)instname:Sociedade Brasileira de Patologia (SBP)instacron:SBP10.5935/1676-2444.20140014info:eu-repo/semantics/openAccessViana,Karina AugustaCarvalho,Maria das GraçasDusse,Luci Maria Sant'AnaFernandes,Aline CaldeiraAvelar,Renato SathlerAvelar,Danielle Marquete VitelliCarvalho,BeatrizRibeiro,Claudia Maria FrancoAntonelli,Lis Ribeiro do ValleTeixeira,AndreaMartins Filho,Olindo Assiseng2014-10-02T00:00:00Zoai:scielo:S1676-24442014000300189Revistahttp://www.scielo.br/jbpmlhttps://old.scielo.br/oai/scielo-oai.php||jbpml@sbpc.org.br1678-47741676-2444opendoar:2014-10-02T00:00Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP)false |
dc.title.none.fl_str_mv |
Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol |
title |
Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol |
spellingShingle |
Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol Viana,Karina Augusta reticulocyte counting methods flow cytometry acridine orange manual reticulocyte count |
title_short |
Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol |
title_full |
Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol |
title_fullStr |
Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol |
title_full_unstemmed |
Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol |
title_sort |
Flow cytometry reticulocyte counting using acridine orange: validation of a new protocol |
author |
Viana,Karina Augusta |
author_facet |
Viana,Karina Augusta Carvalho,Maria das Graças Dusse,Luci Maria Sant'Ana Fernandes,Aline Caldeira Avelar,Renato Sathler Avelar,Danielle Marquete Vitelli Carvalho,Beatriz Ribeiro,Claudia Maria Franco Antonelli,Lis Ribeiro do Valle Teixeira,Andrea Martins Filho,Olindo Assis |
author_role |
author |
author2 |
Carvalho,Maria das Graças Dusse,Luci Maria Sant'Ana Fernandes,Aline Caldeira Avelar,Renato Sathler Avelar,Danielle Marquete Vitelli Carvalho,Beatriz Ribeiro,Claudia Maria Franco Antonelli,Lis Ribeiro do Valle Teixeira,Andrea Martins Filho,Olindo Assis |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Viana,Karina Augusta Carvalho,Maria das Graças Dusse,Luci Maria Sant'Ana Fernandes,Aline Caldeira Avelar,Renato Sathler Avelar,Danielle Marquete Vitelli Carvalho,Beatriz Ribeiro,Claudia Maria Franco Antonelli,Lis Ribeiro do Valle Teixeira,Andrea Martins Filho,Olindo Assis |
dc.subject.por.fl_str_mv |
reticulocyte counting methods flow cytometry acridine orange manual reticulocyte count |
topic |
reticulocyte counting methods flow cytometry acridine orange manual reticulocyte count |
description |
Introduction: Currently, the reticulocyte counting is a challenge for clinical laboratories in Brazil, mainly for the ordinary ones, which still use the manual method. This method has some limitations, since it consists of a laborious method, time consuming, with low accuracy. Objectives: This study has developed and evaluated the performance of a New Laboratory Protocol for flow cytometry (FC) reticulocytes counting using acridine orange (AO) as dye, aiming to standardize a more precise, easy, fast implementation, and low cost protocol. After standardization of the New Protocol (FC/AO), it was compared with the manual method. The results were analyzed according to the recommendations of the National Committee for Clinical Laboratory Standards (NCCLS), now known as Clinical and Laboratory Standards Institute (CLSI), to evaluate the interchangeability of methods in linear regression analysis and paired t test, besides other quality control tests. Conclusion: Based on these results concerning to the correlation between the methods and the tests related to quality control, we can admit that FC/AO for reticulocyte counting shows undeniable advantages when compared to the preexisting manual method. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000300189 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442014000300189 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1676-2444.20140014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Patologia Clínica |
dc.source.none.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial v.50 n.3 2014 reponame:Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) instname:Sociedade Brasileira de Patologia (SBP) instacron:SBP |
instname_str |
Sociedade Brasileira de Patologia (SBP) |
instacron_str |
SBP |
institution |
SBP |
reponame_str |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
collection |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) |
repository.name.fl_str_mv |
Jornal Brasileiro de Patologia e Medicina Laboratorial (Online) - Sociedade Brasileira de Patologia (SBP) |
repository.mail.fl_str_mv |
||jbpml@sbpc.org.br |
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1752122295928750080 |