Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100263 |
Resumo: | Abstract: The osteolytic activity of odontogenic cysts and tumors is directly associated with their growth and aggressiveness. The influence of proteins expressed by epithelial and mesenchymal cells on this biological event differs between indolent cystic lesions, aggressive cystic lesions, and odontogenic tumors. The objective of this study was to compare the immunohistochemical expression of factors that stimulate (receptor activator of nuclear factor kappa-Β ligand – RANKL, cathepsin K – CatK and matrix metallopeptidase 8 – MMP-8) and inhibit (osteoprotegerin – OPG) osteoclastogenesis between dentigerous cyst (DC), glandular odontogenic cyst (GOC), odontogenic keratocyst (OKC), and ameloblastoma (AB). Paraffin-embedded sections of nine DCs, nine GOCs, 20 OKCs, 21 ABs, and four dental follicles (DFs) were subjected to immunohistochemistry. Immunoreactivity was analyzed semiquantitatively and quantitatively in epithelium and connective tissue, respectively. The proteins were immunoexpressed in epithelial and mesenchymal cells of all lesions studied. The expression of RANKL and CatK was higher in OKC, AB, and GOC (p<0.005). Higher expression of OPG was found in DF and DC compared to the other markers (p<0.005). MMP-8 expression was high in GOC and OKC. This study demonstrated the differential expression of factors that inhibit and stimulate bone resorption during the development of DC, GOC, OKC, and AB. Higher expression of RANKL and CatK was observed in more aggressive lesions. OPG appears to be one of the molecules responsible for the slower growth of DC. |
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Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumorsBone ResorptionBone CystsAmeloblastomaBiomarkersImmunohistochemistryAbstract: The osteolytic activity of odontogenic cysts and tumors is directly associated with their growth and aggressiveness. The influence of proteins expressed by epithelial and mesenchymal cells on this biological event differs between indolent cystic lesions, aggressive cystic lesions, and odontogenic tumors. The objective of this study was to compare the immunohistochemical expression of factors that stimulate (receptor activator of nuclear factor kappa-Β ligand – RANKL, cathepsin K – CatK and matrix metallopeptidase 8 – MMP-8) and inhibit (osteoprotegerin – OPG) osteoclastogenesis between dentigerous cyst (DC), glandular odontogenic cyst (GOC), odontogenic keratocyst (OKC), and ameloblastoma (AB). Paraffin-embedded sections of nine DCs, nine GOCs, 20 OKCs, 21 ABs, and four dental follicles (DFs) were subjected to immunohistochemistry. Immunoreactivity was analyzed semiquantitatively and quantitatively in epithelium and connective tissue, respectively. The proteins were immunoexpressed in epithelial and mesenchymal cells of all lesions studied. The expression of RANKL and CatK was higher in OKC, AB, and GOC (p<0.005). Higher expression of OPG was found in DF and DC compared to the other markers (p<0.005). MMP-8 expression was high in GOC and OKC. This study demonstrated the differential expression of factors that inhibit and stimulate bone resorption during the development of DC, GOC, OKC, and AB. Higher expression of RANKL and CatK was observed in more aggressive lesions. OPG appears to be one of the molecules responsible for the slower growth of DC.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100263Brazilian Oral Research v.36 2022reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/1807-3107bor-2022.vol36.0072info:eu-repo/semantics/openAccessAlmeida Junior,Vildeman Rodrigues deLeite,Eder Gerardo SantosAlmeida,Marcus ViniciusCastro,Jurema Freire Lisboa deFreitas,Roseana de AlmeidaXavier,Flávia Caló AquinoFigueiredo,Andreia LealSantos,Jean NunesHenriques,Águida Cristina Gomeseng2022-04-28T00:00:00Zoai:scielo:S1806-83242022000100263Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2022-04-28T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors |
title |
Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors |
spellingShingle |
Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors Almeida Junior,Vildeman Rodrigues de Bone Resorption Bone Cysts Ameloblastoma Biomarkers Immunohistochemistry |
title_short |
Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors |
title_full |
Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors |
title_fullStr |
Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors |
title_full_unstemmed |
Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors |
title_sort |
Differential protein expression of osteoclastogenic factors in odontogenic cysts and tumors |
author |
Almeida Junior,Vildeman Rodrigues de |
author_facet |
Almeida Junior,Vildeman Rodrigues de Leite,Eder Gerardo Santos Almeida,Marcus Vinicius Castro,Jurema Freire Lisboa de Freitas,Roseana de Almeida Xavier,Flávia Caló Aquino Figueiredo,Andreia Leal Santos,Jean Nunes Henriques,Águida Cristina Gomes |
author_role |
author |
author2 |
Leite,Eder Gerardo Santos Almeida,Marcus Vinicius Castro,Jurema Freire Lisboa de Freitas,Roseana de Almeida Xavier,Flávia Caló Aquino Figueiredo,Andreia Leal Santos,Jean Nunes Henriques,Águida Cristina Gomes |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Almeida Junior,Vildeman Rodrigues de Leite,Eder Gerardo Santos Almeida,Marcus Vinicius Castro,Jurema Freire Lisboa de Freitas,Roseana de Almeida Xavier,Flávia Caló Aquino Figueiredo,Andreia Leal Santos,Jean Nunes Henriques,Águida Cristina Gomes |
dc.subject.por.fl_str_mv |
Bone Resorption Bone Cysts Ameloblastoma Biomarkers Immunohistochemistry |
topic |
Bone Resorption Bone Cysts Ameloblastoma Biomarkers Immunohistochemistry |
description |
Abstract: The osteolytic activity of odontogenic cysts and tumors is directly associated with their growth and aggressiveness. The influence of proteins expressed by epithelial and mesenchymal cells on this biological event differs between indolent cystic lesions, aggressive cystic lesions, and odontogenic tumors. The objective of this study was to compare the immunohistochemical expression of factors that stimulate (receptor activator of nuclear factor kappa-Β ligand – RANKL, cathepsin K – CatK and matrix metallopeptidase 8 – MMP-8) and inhibit (osteoprotegerin – OPG) osteoclastogenesis between dentigerous cyst (DC), glandular odontogenic cyst (GOC), odontogenic keratocyst (OKC), and ameloblastoma (AB). Paraffin-embedded sections of nine DCs, nine GOCs, 20 OKCs, 21 ABs, and four dental follicles (DFs) were subjected to immunohistochemistry. Immunoreactivity was analyzed semiquantitatively and quantitatively in epithelium and connective tissue, respectively. The proteins were immunoexpressed in epithelial and mesenchymal cells of all lesions studied. The expression of RANKL and CatK was higher in OKC, AB, and GOC (p<0.005). Higher expression of OPG was found in DF and DC compared to the other markers (p<0.005). MMP-8 expression was high in GOC and OKC. This study demonstrated the differential expression of factors that inhibit and stimulate bone resorption during the development of DC, GOC, OKC, and AB. Higher expression of RANKL and CatK was observed in more aggressive lesions. OPG appears to be one of the molecules responsible for the slower growth of DC. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100263 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242022000100263 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1807-3107bor-2022.vol36.0072 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.36 2022 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
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1750318328422858752 |