Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

Detalhes bibliográficos
Autor(a) principal: Ribeiro,Daniel Araki
Data de Publicação: 2004
Outros Autores: Scolastici,Clarissa, Marques,Mariângela Esther Alencar, Salvadori,Daisy Maria Fávero
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000300003
Resumo: Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.
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spelling Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitroSodium fluorideComet assayMutagenicity testsFluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000300003Brazilian Oral Research v.18 n.3 2004reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242004000300003info:eu-repo/semantics/openAccessRibeiro,Daniel ArakiScolastici,ClarissaMarques,Mariângela Esther AlencarSalvadori,Daisy Maria Fáveroeng2004-09-29T00:00:00Zoai:scielo:S1806-83242004000300003Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2004-09-29T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
title Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
spellingShingle Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
Ribeiro,Daniel Araki
Sodium fluoride
Comet assay
Mutagenicity tests
title_short Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
title_full Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
title_fullStr Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
title_full_unstemmed Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
title_sort Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
author Ribeiro,Daniel Araki
author_facet Ribeiro,Daniel Araki
Scolastici,Clarissa
Marques,Mariângela Esther Alencar
Salvadori,Daisy Maria Fávero
author_role author
author2 Scolastici,Clarissa
Marques,Mariângela Esther Alencar
Salvadori,Daisy Maria Fávero
author2_role author
author
author
dc.contributor.author.fl_str_mv Ribeiro,Daniel Araki
Scolastici,Clarissa
Marques,Mariângela Esther Alencar
Salvadori,Daisy Maria Fávero
dc.subject.por.fl_str_mv Sodium fluoride
Comet assay
Mutagenicity tests
topic Sodium fluoride
Comet assay
Mutagenicity tests
description Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.
publishDate 2004
dc.date.none.fl_str_mv 2004-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000300003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000300003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1806-83242004000300003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.18 n.3 2004
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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