Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242004000300003 http://hdl.handle.net/11449/67789 |
Resumo: | Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 micro/ml for 3 h, at 37 dgrees C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents. |
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Repositório Institucional da UNESP |
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Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.fluoride sodiumanimalCHO cellcomet assayCricetulusDNA damagedrug effectfemalehamsterAnimalsCHO CellsComet AssayCricetinaeDNA DamageFemaleSodium FluorideFluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 micro/ml for 3 h, at 37 dgrees C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.Universidade Estadual Paulista (Unesp)Ribeiro, Daniel ArakiScolastici, ClarissaMarques, Mariângela Esther Alencar [UNESP]Salvadori, Daisy Maria Favero [UNESP]2014-05-27T11:21:06Z2014-05-27T11:21:06Z2004-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article192-196application/pdfhttp://dx.doi.org/10.1590/S1806-83242004000300003Pesquisa odontologica brasileira = Brazilian oral research, v. 18, n. 3, p. 192-196, 2004.1806-8324http://hdl.handle.net/11449/6778910.1590/S1806-83242004000300003S1806-832420040003000032-s2.0-237445124552-s2.0-23744512455.pdf50511187529809037528116925519142Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Odontológica Brasileira = Brazilian Oral Researchinfo:eu-repo/semantics/openAccess2024-09-03T13:14:31Zoai:repositorio.unesp.br:11449/67789Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro. |
title |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro. |
spellingShingle |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro. Ribeiro, Daniel Araki fluoride sodium animal CHO cell comet assay Cricetulus DNA damage drug effect female hamster Animals CHO Cells Comet Assay Cricetinae DNA Damage Female Sodium Fluoride |
title_short |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro. |
title_full |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro. |
title_fullStr |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro. |
title_full_unstemmed |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro. |
title_sort |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro. |
author |
Ribeiro, Daniel Araki |
author_facet |
Ribeiro, Daniel Araki Scolastici, Clarissa Marques, Mariângela Esther Alencar [UNESP] Salvadori, Daisy Maria Favero [UNESP] |
author_role |
author |
author2 |
Scolastici, Clarissa Marques, Mariângela Esther Alencar [UNESP] Salvadori, Daisy Maria Favero [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ribeiro, Daniel Araki Scolastici, Clarissa Marques, Mariângela Esther Alencar [UNESP] Salvadori, Daisy Maria Favero [UNESP] |
dc.subject.por.fl_str_mv |
fluoride sodium animal CHO cell comet assay Cricetulus DNA damage drug effect female hamster Animals CHO Cells Comet Assay Cricetinae DNA Damage Female Sodium Fluoride |
topic |
fluoride sodium animal CHO cell comet assay Cricetulus DNA damage drug effect female hamster Animals CHO Cells Comet Assay Cricetinae DNA Damage Female Sodium Fluoride |
description |
Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 micro/ml for 3 h, at 37 dgrees C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-07-01 2014-05-27T11:21:06Z 2014-05-27T11:21:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242004000300003 Pesquisa odontologica brasileira = Brazilian oral research, v. 18, n. 3, p. 192-196, 2004. 1806-8324 http://hdl.handle.net/11449/67789 10.1590/S1806-83242004000300003 S1806-83242004000300003 2-s2.0-23744512455 2-s2.0-23744512455.pdf 5051118752980903 7528116925519142 |
url |
http://dx.doi.org/10.1590/S1806-83242004000300003 http://hdl.handle.net/11449/67789 |
identifier_str_mv |
Pesquisa odontologica brasileira = Brazilian oral research, v. 18, n. 3, p. 192-196, 2004. 1806-8324 10.1590/S1806-83242004000300003 S1806-83242004000300003 2-s2.0-23744512455 2-s2.0-23744512455.pdf 5051118752980903 7528116925519142 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Odontológica Brasileira = Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
192-196 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021362871304192 |