Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Daniel Araki
Data de Publicação: 2004
Outros Autores: Scolastici, Clarissa, Marques, Mariângela Esther Alencar [UNESP], Salvadori, Daisy Maria Favero [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1806-83242004000300003
http://hdl.handle.net/11449/67789
Resumo: Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 micro/ml for 3 h, at 37 dgrees C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.
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spelling Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.fluoride sodiumanimalCHO cellcomet assayCricetulusDNA damagedrug effectfemalehamsterAnimalsCHO CellsComet AssayCricetinaeDNA DamageFemaleSodium FluorideFluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 micro/ml for 3 h, at 37 dgrees C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.Universidade Estadual Paulista (Unesp)Ribeiro, Daniel ArakiScolastici, ClarissaMarques, Mariângela Esther Alencar [UNESP]Salvadori, Daisy Maria Favero [UNESP]2014-05-27T11:21:06Z2014-05-27T11:21:06Z2004-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article192-196application/pdfhttp://dx.doi.org/10.1590/S1806-83242004000300003Pesquisa odontologica brasileira = Brazilian oral research, v. 18, n. 3, p. 192-196, 2004.1806-8324http://hdl.handle.net/11449/6778910.1590/S1806-83242004000300003S1806-832420040003000032-s2.0-237445124552-s2.0-23744512455.pdf50511187529809037528116925519142Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Odontológica Brasileira = Brazilian Oral Researchinfo:eu-repo/semantics/openAccess2024-09-03T13:14:31Zoai:repositorio.unesp.br:11449/67789Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
title Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
spellingShingle Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
Ribeiro, Daniel Araki
fluoride sodium
animal
CHO cell
comet assay
Cricetulus
DNA damage
drug effect
female
hamster
Animals
CHO Cells
Comet Assay
Cricetinae
DNA Damage
Female
Sodium Fluoride
title_short Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
title_full Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
title_fullStr Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
title_full_unstemmed Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
title_sort Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro.
author Ribeiro, Daniel Araki
author_facet Ribeiro, Daniel Araki
Scolastici, Clarissa
Marques, Mariângela Esther Alencar [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
author_role author
author2 Scolastici, Clarissa
Marques, Mariângela Esther Alencar [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ribeiro, Daniel Araki
Scolastici, Clarissa
Marques, Mariângela Esther Alencar [UNESP]
Salvadori, Daisy Maria Favero [UNESP]
dc.subject.por.fl_str_mv fluoride sodium
animal
CHO cell
comet assay
Cricetulus
DNA damage
drug effect
female
hamster
Animals
CHO Cells
Comet Assay
Cricetinae
DNA Damage
Female
Sodium Fluoride
topic fluoride sodium
animal
CHO cell
comet assay
Cricetulus
DNA damage
drug effect
female
hamster
Animals
CHO Cells
Comet Assay
Cricetinae
DNA Damage
Female
Sodium Fluoride
description Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 micro/ml for 3 h, at 37 dgrees C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.
publishDate 2004
dc.date.none.fl_str_mv 2004-07-01
2014-05-27T11:21:06Z
2014-05-27T11:21:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1806-83242004000300003
Pesquisa odontologica brasileira = Brazilian oral research, v. 18, n. 3, p. 192-196, 2004.
1806-8324
http://hdl.handle.net/11449/67789
10.1590/S1806-83242004000300003
S1806-83242004000300003
2-s2.0-23744512455
2-s2.0-23744512455.pdf
5051118752980903
7528116925519142
url http://dx.doi.org/10.1590/S1806-83242004000300003
http://hdl.handle.net/11449/67789
identifier_str_mv Pesquisa odontologica brasileira = Brazilian oral research, v. 18, n. 3, p. 192-196, 2004.
1806-8324
10.1590/S1806-83242004000300003
S1806-83242004000300003
2-s2.0-23744512455
2-s2.0-23744512455.pdf
5051118752980903
7528116925519142
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Odontológica Brasileira = Brazilian Oral Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 192-196
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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