Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Oral Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000300003 |
Resumo: | Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents. |
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Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitroSodium fluorideComet assayMutagenicity testsFluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000300003Brazilian Oral Research v.18 n.3 2004reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242004000300003info:eu-repo/semantics/openAccessRibeiro,Daniel ArakiScolastici,ClarissaMarques,Mariângela Esther AlencarSalvadori,Daisy Maria Fáveroeng2004-09-29T00:00:00Zoai:scielo:S1806-83242004000300003Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2004-09-29T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false |
dc.title.none.fl_str_mv |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro |
title |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro |
spellingShingle |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro Ribeiro,Daniel Araki Sodium fluoride Comet assay Mutagenicity tests |
title_short |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro |
title_full |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro |
title_fullStr |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro |
title_full_unstemmed |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro |
title_sort |
Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro |
author |
Ribeiro,Daniel Araki |
author_facet |
Ribeiro,Daniel Araki Scolastici,Clarissa Marques,Mariângela Esther Alencar Salvadori,Daisy Maria Fávero |
author_role |
author |
author2 |
Scolastici,Clarissa Marques,Mariângela Esther Alencar Salvadori,Daisy Maria Fávero |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ribeiro,Daniel Araki Scolastici,Clarissa Marques,Mariângela Esther Alencar Salvadori,Daisy Maria Fávero |
dc.subject.por.fl_str_mv |
Sodium fluoride Comet assay Mutagenicity tests |
topic |
Sodium fluoride Comet assay Mutagenicity tests |
description |
Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000300003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242004000300003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-83242004000300003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
Brazilian Oral Research v.18 n.3 2004 reponame:Brazilian Oral Research instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO |
instname_str |
Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
instacron_str |
SBPQO |
institution |
SBPQO |
reponame_str |
Brazilian Oral Research |
collection |
Brazilian Oral Research |
repository.name.fl_str_mv |
Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO) |
repository.mail.fl_str_mv |
pob@edu.usp.br||bor@sbpqo.org.br |
_version_ |
1750318320306880512 |