Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun

Detalhes bibliográficos
Autor(a) principal: Jiang,Xinyu
Data de Publicação: 2010
Outros Autores: Shi,Shuyun, Zhang,Yuping, Chen,Xiaoqing
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800017
Resumo: Our previous research found that the crude extract of Neo-T. siphonanthun exhibited an effective DPPH (1,1-diphenyl-2-picryhydrazyl) radical scavenging activity. In this study an online rapid screening method, high-performance liquid chromatography-radical scavenging detection-diode array detector-electrospray ionization mass spectrometry (HPLC-RSD- DAD-ESI/MS) system, was developed for screening individual antioxidants from the most active fraction. Accordingly, three isomeric derivatives were detected. The target active compounds were isolated by high-speed counter-current chromatography (HSCCC) with the purity over 99%, and were identified as luteolin-3'-O-β-D-glucopyranoside (1), luteolin-7-O-β-D-glucopyranoside (2) and luteolin-4'-O-β-D-glucopyranoside (3) by analysis of its off-line nuclear magnetic resonance (NMR) spectra. Antioxidant activity of three compounds was assessed by off-line DPPH assay, and all of them showed potent activity.
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spelling Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthunHPLC-RSD-DAD-ESI/MSradical scavengerflavonoidHSCCCNeo-Taraxacum siphonanthunOur previous research found that the crude extract of Neo-T. siphonanthun exhibited an effective DPPH (1,1-diphenyl-2-picryhydrazyl) radical scavenging activity. In this study an online rapid screening method, high-performance liquid chromatography-radical scavenging detection-diode array detector-electrospray ionization mass spectrometry (HPLC-RSD- DAD-ESI/MS) system, was developed for screening individual antioxidants from the most active fraction. Accordingly, three isomeric derivatives were detected. The target active compounds were isolated by high-speed counter-current chromatography (HSCCC) with the purity over 99%, and were identified as luteolin-3'-O-β-D-glucopyranoside (1), luteolin-7-O-β-D-glucopyranoside (2) and luteolin-4'-O-β-D-glucopyranoside (3) by analysis of its off-line nuclear magnetic resonance (NMR) spectra. Antioxidant activity of three compounds was assessed by off-line DPPH assay, and all of them showed potent activity.Sociedade Brasileira de Química2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800017Journal of the Brazilian Chemical Society v.21 n.8 2010reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532010000800017info:eu-repo/semantics/openAccessJiang,XinyuShi,ShuyunZhang,YupingChen,Xiaoqingeng2011-10-14T00:00:00Zoai:scielo:S0103-50532010000800017Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2011-10-14T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun
title Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun
spellingShingle Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun
Jiang,Xinyu
HPLC-RSD-DAD-ESI/MS
radical scavenger
flavonoid
HSCCC
Neo-Taraxacum siphonanthun
title_short Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun
title_full Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun
title_fullStr Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun
title_full_unstemmed Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun
title_sort Excellent combination of HPLC-RSD-DAD-ESI/MS and HSCCC experiments to screen and identify radical scavengers from Neo-Taraxacum siphonanthun
author Jiang,Xinyu
author_facet Jiang,Xinyu
Shi,Shuyun
Zhang,Yuping
Chen,Xiaoqing
author_role author
author2 Shi,Shuyun
Zhang,Yuping
Chen,Xiaoqing
author2_role author
author
author
dc.contributor.author.fl_str_mv Jiang,Xinyu
Shi,Shuyun
Zhang,Yuping
Chen,Xiaoqing
dc.subject.por.fl_str_mv HPLC-RSD-DAD-ESI/MS
radical scavenger
flavonoid
HSCCC
Neo-Taraxacum siphonanthun
topic HPLC-RSD-DAD-ESI/MS
radical scavenger
flavonoid
HSCCC
Neo-Taraxacum siphonanthun
description Our previous research found that the crude extract of Neo-T. siphonanthun exhibited an effective DPPH (1,1-diphenyl-2-picryhydrazyl) radical scavenging activity. In this study an online rapid screening method, high-performance liquid chromatography-radical scavenging detection-diode array detector-electrospray ionization mass spectrometry (HPLC-RSD- DAD-ESI/MS) system, was developed for screening individual antioxidants from the most active fraction. Accordingly, three isomeric derivatives were detected. The target active compounds were isolated by high-speed counter-current chromatography (HSCCC) with the purity over 99%, and were identified as luteolin-3'-O-β-D-glucopyranoside (1), luteolin-7-O-β-D-glucopyranoside (2) and luteolin-4'-O-β-D-glucopyranoside (3) by analysis of its off-line nuclear magnetic resonance (NMR) spectra. Antioxidant activity of three compounds was assessed by off-line DPPH assay, and all of them showed potent activity.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000800017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532010000800017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.21 n.8 2010
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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