Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system
Autor(a) principal: | |
---|---|
Data de Publicação: | 1997 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50531997000100009 |
Resumo: | A flow-injection (FI) method was developed for the determination of oxalate in urine. It was based on the use of oxalate oxidase (E.C. 1.2.3.4) immobilized on ground seeds of the BR-303 Sorghum vulgare variety. A reactor was filled with this activated material, and the samples (200 µL) containing oxalate were passed through it, carried by a deionized water flow. The carbon dioxide produced by the enzyme reaction permeated through a microporous PTFE membrane, and was received in a water acceptor stream, promoting conductivity changes proportional to the oxalate concentration in the sample. The results obtained showed a useful linear range from 0.05 to 0.50 mmol dm-3. The proposed method, when compared with the Sigma enzymatic procedure, showed good correlation (Y = 0.006( + 0.016) + 0.98( + 0.019)X; r = 0.9995, Y = conductivity in µS, and X = concentration in mmol dm-3), selectivity, and sensitivity. The new immobilization approach promotes greater stability, allowing oxalate determination for 6 months. About 13 determinations can be performed per hour. The precision of the proposed method is about + 3.2 % (r.s.d). |
id |
SBQ-2_380d16d214ac59b2a2e7cd0998e7f44e |
---|---|
oai_identifier_str |
oai:scielo:S0103-50531997000100009 |
network_acronym_str |
SBQ-2 |
network_name_str |
Journal of the Brazilian Chemical Society (Online) |
repository_id_str |
|
spelling |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric systemflow injectionoxalate oxidaseoxalate determinationenzyme immobilizationSorghum vulgareconductimetryA flow-injection (FI) method was developed for the determination of oxalate in urine. It was based on the use of oxalate oxidase (E.C. 1.2.3.4) immobilized on ground seeds of the BR-303 Sorghum vulgare variety. A reactor was filled with this activated material, and the samples (200 µL) containing oxalate were passed through it, carried by a deionized water flow. The carbon dioxide produced by the enzyme reaction permeated through a microporous PTFE membrane, and was received in a water acceptor stream, promoting conductivity changes proportional to the oxalate concentration in the sample. The results obtained showed a useful linear range from 0.05 to 0.50 mmol dm-3. The proposed method, when compared with the Sigma enzymatic procedure, showed good correlation (Y = 0.006( + 0.016) + 0.98( + 0.019)X; r = 0.9995, Y = conductivity in µS, and X = concentration in mmol dm-3), selectivity, and sensitivity. The new immobilization approach promotes greater stability, allowing oxalate determination for 6 months. About 13 determinations can be performed per hour. The precision of the proposed method is about + 3.2 % (r.s.d).Sociedade Brasileira de Química1997-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50531997000100009Journal of the Brazilian Chemical Society v.8 n.1 1997reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50531997000100009info:eu-repo/semantics/openAccessOliveira Neto,Graciliano deTubino,MatthieuGodinho,Oswaldo Espirito SantoKubota,Lauro TatsuoFernandes,João Robertoeng2011-10-04T00:00:00Zoai:scielo:S0103-50531997000100009Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2011-10-04T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system |
title |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system |
spellingShingle |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system Oliveira Neto,Graciliano de flow injection oxalate oxidase oxalate determination enzyme immobilization Sorghum vulgare conductimetry |
title_short |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system |
title_full |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system |
title_fullStr |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system |
title_full_unstemmed |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system |
title_sort |
Oxalate determination in urine using an immobilized enzyme on sorghum vulgare seeds in a flow injection conductimetric system |
author |
Oliveira Neto,Graciliano de |
author_facet |
Oliveira Neto,Graciliano de Tubino,Matthieu Godinho,Oswaldo Espirito Santo Kubota,Lauro Tatsuo Fernandes,João Roberto |
author_role |
author |
author2 |
Tubino,Matthieu Godinho,Oswaldo Espirito Santo Kubota,Lauro Tatsuo Fernandes,João Roberto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Oliveira Neto,Graciliano de Tubino,Matthieu Godinho,Oswaldo Espirito Santo Kubota,Lauro Tatsuo Fernandes,João Roberto |
dc.subject.por.fl_str_mv |
flow injection oxalate oxidase oxalate determination enzyme immobilization Sorghum vulgare conductimetry |
topic |
flow injection oxalate oxidase oxalate determination enzyme immobilization Sorghum vulgare conductimetry |
description |
A flow-injection (FI) method was developed for the determination of oxalate in urine. It was based on the use of oxalate oxidase (E.C. 1.2.3.4) immobilized on ground seeds of the BR-303 Sorghum vulgare variety. A reactor was filled with this activated material, and the samples (200 µL) containing oxalate were passed through it, carried by a deionized water flow. The carbon dioxide produced by the enzyme reaction permeated through a microporous PTFE membrane, and was received in a water acceptor stream, promoting conductivity changes proportional to the oxalate concentration in the sample. The results obtained showed a useful linear range from 0.05 to 0.50 mmol dm-3. The proposed method, when compared with the Sigma enzymatic procedure, showed good correlation (Y = 0.006( + 0.016) + 0.98( + 0.019)X; r = 0.9995, Y = conductivity in µS, and X = concentration in mmol dm-3), selectivity, and sensitivity. The new immobilization approach promotes greater stability, allowing oxalate determination for 6 months. About 13 determinations can be performed per hour. The precision of the proposed method is about + 3.2 % (r.s.d). |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50531997000100009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50531997000100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50531997000100009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.8 n.1 1997 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318162977488896 |