Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700028 |
Resumo: | Photochemical derivatization was proposed to enable intense fluorescence (412/465 nm) from erythromycin. Crucial experimental parameters such as type and concentration of the acid used to treat analyte solutions and UV irradiation time were adjusted. Limits of detection and quantification of 0.025 and 0.085 µg mL-1 were achieved with linear range up to 200 µg mL-1. The procedure was selective towards the presence of aminoglycoside antibiotics (kanamycin, gentamycin and amikacin). The method was tested using pharmaceutical formulations and one vaccine composition containing erythromycin as a preservative component, with analyte recoveries between 98 and 105%. |
id |
SBQ-2_6af0a436e6e974fd2fd091baa8192e85 |
---|---|
oai_identifier_str |
oai:scielo:S0103-50532008000700028 |
network_acronym_str |
SBQ-2 |
network_name_str |
Journal of the Brazilian Chemical Society (Online) |
repository_id_str |
|
spelling |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approachphotochemical induced fluorescenceerythromycinpharmaceutical formulationsPhotochemical derivatization was proposed to enable intense fluorescence (412/465 nm) from erythromycin. Crucial experimental parameters such as type and concentration of the acid used to treat analyte solutions and UV irradiation time were adjusted. Limits of detection and quantification of 0.025 and 0.085 µg mL-1 were achieved with linear range up to 200 µg mL-1. The procedure was selective towards the presence of aminoglycoside antibiotics (kanamycin, gentamycin and amikacin). The method was tested using pharmaceutical formulations and one vaccine composition containing erythromycin as a preservative component, with analyte recoveries between 98 and 105%.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700028Journal of the Brazilian Chemical Society v.19 n.7 2008reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532008000700028info:eu-repo/semantics/openAccessFinete,Virginia de L. M.Arissawa,MárciaAucélio,Ricardo Q.eng2017-05-05T00:00:00Zoai:scielo:S0103-50532008000700028Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-05-05T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach |
title |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach |
spellingShingle |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach Finete,Virginia de L. M. photochemical induced fluorescence erythromycin pharmaceutical formulations |
title_short |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach |
title_full |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach |
title_fullStr |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach |
title_full_unstemmed |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach |
title_sort |
Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach |
author |
Finete,Virginia de L. M. |
author_facet |
Finete,Virginia de L. M. Arissawa,Márcia Aucélio,Ricardo Q. |
author_role |
author |
author2 |
Arissawa,Márcia Aucélio,Ricardo Q. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Finete,Virginia de L. M. Arissawa,Márcia Aucélio,Ricardo Q. |
dc.subject.por.fl_str_mv |
photochemical induced fluorescence erythromycin pharmaceutical formulations |
topic |
photochemical induced fluorescence erythromycin pharmaceutical formulations |
description |
Photochemical derivatization was proposed to enable intense fluorescence (412/465 nm) from erythromycin. Crucial experimental parameters such as type and concentration of the acid used to treat analyte solutions and UV irradiation time were adjusted. Limits of detection and quantification of 0.025 and 0.085 µg mL-1 were achieved with linear range up to 200 µg mL-1. The procedure was selective towards the presence of aminoglycoside antibiotics (kanamycin, gentamycin and amikacin). The method was tested using pharmaceutical formulations and one vaccine composition containing erythromycin as a preservative component, with analyte recoveries between 98 and 105%. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700028 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700028 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532008000700028 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.19 n.7 2008 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318169372753920 |