Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach

Detalhes bibliográficos
Autor(a) principal: Finete,Virginia de L. M.
Data de Publicação: 2008
Outros Autores: Arissawa,Márcia, Aucélio,Ricardo Q.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700028
Resumo: Photochemical derivatization was proposed to enable intense fluorescence (412/465 nm) from erythromycin. Crucial experimental parameters such as type and concentration of the acid used to treat analyte solutions and UV irradiation time were adjusted. Limits of detection and quantification of 0.025 and 0.085 µg mL-1 were achieved with linear range up to 200 µg mL-1. The procedure was selective towards the presence of aminoglycoside antibiotics (kanamycin, gentamycin and amikacin). The method was tested using pharmaceutical formulations and one vaccine composition containing erythromycin as a preservative component, with analyte recoveries between 98 and 105%.
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spelling Fluorimetric method for the determination of erythromycin using a photochemical derivatization approachphotochemical induced fluorescenceerythromycinpharmaceutical formulationsPhotochemical derivatization was proposed to enable intense fluorescence (412/465 nm) from erythromycin. Crucial experimental parameters such as type and concentration of the acid used to treat analyte solutions and UV irradiation time were adjusted. Limits of detection and quantification of 0.025 and 0.085 µg mL-1 were achieved with linear range up to 200 µg mL-1. The procedure was selective towards the presence of aminoglycoside antibiotics (kanamycin, gentamycin and amikacin). The method was tested using pharmaceutical formulations and one vaccine composition containing erythromycin as a preservative component, with analyte recoveries between 98 and 105%.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700028Journal of the Brazilian Chemical Society v.19 n.7 2008reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532008000700028info:eu-repo/semantics/openAccessFinete,Virginia de L. M.Arissawa,MárciaAucélio,Ricardo Q.eng2017-05-05T00:00:00Zoai:scielo:S0103-50532008000700028Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-05-05T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
title Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
spellingShingle Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
Finete,Virginia de L. M.
photochemical induced fluorescence
erythromycin
pharmaceutical formulations
title_short Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
title_full Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
title_fullStr Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
title_full_unstemmed Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
title_sort Fluorimetric method for the determination of erythromycin using a photochemical derivatization approach
author Finete,Virginia de L. M.
author_facet Finete,Virginia de L. M.
Arissawa,Márcia
Aucélio,Ricardo Q.
author_role author
author2 Arissawa,Márcia
Aucélio,Ricardo Q.
author2_role author
author
dc.contributor.author.fl_str_mv Finete,Virginia de L. M.
Arissawa,Márcia
Aucélio,Ricardo Q.
dc.subject.por.fl_str_mv photochemical induced fluorescence
erythromycin
pharmaceutical formulations
topic photochemical induced fluorescence
erythromycin
pharmaceutical formulations
description Photochemical derivatization was proposed to enable intense fluorescence (412/465 nm) from erythromycin. Crucial experimental parameters such as type and concentration of the acid used to treat analyte solutions and UV irradiation time were adjusted. Limits of detection and quantification of 0.025 and 0.085 µg mL-1 were achieved with linear range up to 200 µg mL-1. The procedure was selective towards the presence of aminoglycoside antibiotics (kanamycin, gentamycin and amikacin). The method was tested using pharmaceutical formulations and one vaccine composition containing erythromycin as a preservative component, with analyte recoveries between 98 and 105%.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700028
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700028
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532008000700028
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.19 n.7 2008
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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