Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000700013 |
Resumo: | An easy and simple chromatographic method for the determination of riboflavin in bovine milk in the presence of tetracyclines was established. For this, the proteins were precipitated with trichloroacetic acid and the separation was carried out by reversed-phase HPLC with fluorescence detection. A C8 column was used with isocratic flow using a mobile phase consisting of 0.1 mol L-1 sodium acetate, 35 mmol L-1calcium chloride and 25 mmol L-1 EDTA : methanol (65:35 v/v). The method was validated by means of the following parameters: linearity (0.9980), linear range (0.50 - 1.30 µg mL-1), intra- and inter-assay precision (RSD < 0.5%), accuracy (recovery 92%), and detection and quantitation limits (0.75 µg mL-1 and 0.22 µg mL-1, respectively). The method was applied for analysis of full cream and skimmed milk, showing robustness in the presence of tetracyclines, and when compared with a spectrofluorimetric method showed no significant difference (p < 0.05) between the results obtained. |
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Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detectionriboflavinmilktetracyclinesAn easy and simple chromatographic method for the determination of riboflavin in bovine milk in the presence of tetracyclines was established. For this, the proteins were precipitated with trichloroacetic acid and the separation was carried out by reversed-phase HPLC with fluorescence detection. A C8 column was used with isocratic flow using a mobile phase consisting of 0.1 mol L-1 sodium acetate, 35 mmol L-1calcium chloride and 25 mmol L-1 EDTA : methanol (65:35 v/v). The method was validated by means of the following parameters: linearity (0.9980), linear range (0.50 - 1.30 µg mL-1), intra- and inter-assay precision (RSD < 0.5%), accuracy (recovery 92%), and detection and quantitation limits (0.75 µg mL-1 and 0.22 µg mL-1, respectively). The method was applied for analysis of full cream and skimmed milk, showing robustness in the presence of tetracyclines, and when compared with a spectrofluorimetric method showed no significant difference (p < 0.05) between the results obtained.Sociedade Brasileira de Química2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000700013Journal of the Brazilian Chemical Society v.16 n.6a 2005reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532005000700013info:eu-repo/semantics/openAccessSilva Jr.,Luiz SeveroTrevisan,Marcello G.Rath,SusannePoppi,Ronei J.Reyes,Felix G. R.eng2006-01-05T00:00:00Zoai:scielo:S0103-50532005000700013Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2006-01-05T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection |
title |
Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection |
spellingShingle |
Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection Silva Jr.,Luiz Severo riboflavin milk tetracyclines |
title_short |
Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection |
title_full |
Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection |
title_fullStr |
Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection |
title_full_unstemmed |
Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection |
title_sort |
Chromatographic determination of riboflavin in the presence of tetracyclines in skimmed and full cream milk using fluorescence detection |
author |
Silva Jr.,Luiz Severo |
author_facet |
Silva Jr.,Luiz Severo Trevisan,Marcello G. Rath,Susanne Poppi,Ronei J. Reyes,Felix G. R. |
author_role |
author |
author2 |
Trevisan,Marcello G. Rath,Susanne Poppi,Ronei J. Reyes,Felix G. R. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva Jr.,Luiz Severo Trevisan,Marcello G. Rath,Susanne Poppi,Ronei J. Reyes,Felix G. R. |
dc.subject.por.fl_str_mv |
riboflavin milk tetracyclines |
topic |
riboflavin milk tetracyclines |
description |
An easy and simple chromatographic method for the determination of riboflavin in bovine milk in the presence of tetracyclines was established. For this, the proteins were precipitated with trichloroacetic acid and the separation was carried out by reversed-phase HPLC with fluorescence detection. A C8 column was used with isocratic flow using a mobile phase consisting of 0.1 mol L-1 sodium acetate, 35 mmol L-1calcium chloride and 25 mmol L-1 EDTA : methanol (65:35 v/v). The method was validated by means of the following parameters: linearity (0.9980), linear range (0.50 - 1.30 µg mL-1), intra- and inter-assay precision (RSD < 0.5%), accuracy (recovery 92%), and detection and quantitation limits (0.75 µg mL-1 and 0.22 µg mL-1, respectively). The method was applied for analysis of full cream and skimmed milk, showing robustness in the presence of tetracyclines, and when compared with a spectrofluorimetric method showed no significant difference (p < 0.05) between the results obtained. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000700013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000700013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532005000700013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.16 n.6a 2005 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318166610804736 |