Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC

Detalhes bibliográficos
Autor(a) principal: Li,Yanfang
Data de Publicação: 2013
Outros Autores: Han,Juan, Wang,Yun, Ma,Jingjing, Yan,Yongsheng, Cao,Lihua
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000400021
Resumo: An aqueous two-phase system (ATPS) based on short chain alcohol and salt was the method for preconcentration, separation and analysis of chloramphenicol (CAM), coupled with high performance liquid chromatography with ultraviolet-visible detector (HPLC-UV). In order to select the suitable ATPS for CAM extraction, different ATPSs were tested and n-propanol/potassium citrate ATPS was chosen for separating and concentrating CAM in the subsequent experiments. The influences of the salt concentration, pH and the volume of n-propanol on the extraction efficiency and partition coefficient of CAM were examined. Response surface methodology was employed to optimize the experimental conditions. Under the optimal conditions, this method has been applied to quantitative determination of CAM in livestock meat samples with limit of detection of 0.48 ng g-1 and limit of quantification of 1.6 ng g-1 with a recovery range of 92.39-104.12%. This ATPS used low cost of organic solvents and supplied a moderate and biocompatible environment, which is suitable for biomolecules.
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spelling Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLCHPLCaqueous two-phase systemextractionchloramphenicollivestock meatATPSAn aqueous two-phase system (ATPS) based on short chain alcohol and salt was the method for preconcentration, separation and analysis of chloramphenicol (CAM), coupled with high performance liquid chromatography with ultraviolet-visible detector (HPLC-UV). In order to select the suitable ATPS for CAM extraction, different ATPSs were tested and n-propanol/potassium citrate ATPS was chosen for separating and concentrating CAM in the subsequent experiments. The influences of the salt concentration, pH and the volume of n-propanol on the extraction efficiency and partition coefficient of CAM were examined. Response surface methodology was employed to optimize the experimental conditions. Under the optimal conditions, this method has been applied to quantitative determination of CAM in livestock meat samples with limit of detection of 0.48 ng g-1 and limit of quantification of 1.6 ng g-1 with a recovery range of 92.39-104.12%. This ATPS used low cost of organic solvents and supplied a moderate and biocompatible environment, which is suitable for biomolecules.Sociedade Brasileira de Química2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000400021Journal of the Brazilian Chemical Society v.24 n.4 2013reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20130088info:eu-repo/semantics/openAccessLi,YanfangHan,JuanWang,YunMa,JingjingYan,YongshengCao,Lihuaeng2013-05-20T00:00:00Zoai:scielo:S0103-50532013000400021Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-05-20T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
title Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
spellingShingle Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
Li,Yanfang
HPLC
aqueous two-phase system
extraction
chloramphenicol
livestock meat
ATPS
title_short Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
title_full Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
title_fullStr Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
title_full_unstemmed Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
title_sort Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
author Li,Yanfang
author_facet Li,Yanfang
Han,Juan
Wang,Yun
Ma,Jingjing
Yan,Yongsheng
Cao,Lihua
author_role author
author2 Han,Juan
Wang,Yun
Ma,Jingjing
Yan,Yongsheng
Cao,Lihua
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Li,Yanfang
Han,Juan
Wang,Yun
Ma,Jingjing
Yan,Yongsheng
Cao,Lihua
dc.subject.por.fl_str_mv HPLC
aqueous two-phase system
extraction
chloramphenicol
livestock meat
ATPS
topic HPLC
aqueous two-phase system
extraction
chloramphenicol
livestock meat
ATPS
description An aqueous two-phase system (ATPS) based on short chain alcohol and salt was the method for preconcentration, separation and analysis of chloramphenicol (CAM), coupled with high performance liquid chromatography with ultraviolet-visible detector (HPLC-UV). In order to select the suitable ATPS for CAM extraction, different ATPSs were tested and n-propanol/potassium citrate ATPS was chosen for separating and concentrating CAM in the subsequent experiments. The influences of the salt concentration, pH and the volume of n-propanol on the extraction efficiency and partition coefficient of CAM were examined. Response surface methodology was employed to optimize the experimental conditions. Under the optimal conditions, this method has been applied to quantitative determination of CAM in livestock meat samples with limit of detection of 0.48 ng g-1 and limit of quantification of 1.6 ng g-1 with a recovery range of 92.39-104.12%. This ATPS used low cost of organic solvents and supplied a moderate and biocompatible environment, which is suitable for biomolecules.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000400021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000400021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20130088
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.24 n.4 2013
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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