Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000400021 |
Resumo: | An aqueous two-phase system (ATPS) based on short chain alcohol and salt was the method for preconcentration, separation and analysis of chloramphenicol (CAM), coupled with high performance liquid chromatography with ultraviolet-visible detector (HPLC-UV). In order to select the suitable ATPS for CAM extraction, different ATPSs were tested and n-propanol/potassium citrate ATPS was chosen for separating and concentrating CAM in the subsequent experiments. The influences of the salt concentration, pH and the volume of n-propanol on the extraction efficiency and partition coefficient of CAM were examined. Response surface methodology was employed to optimize the experimental conditions. Under the optimal conditions, this method has been applied to quantitative determination of CAM in livestock meat samples with limit of detection of 0.48 ng g-1 and limit of quantification of 1.6 ng g-1 with a recovery range of 92.39-104.12%. This ATPS used low cost of organic solvents and supplied a moderate and biocompatible environment, which is suitable for biomolecules. |
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Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLCHPLCaqueous two-phase systemextractionchloramphenicollivestock meatATPSAn aqueous two-phase system (ATPS) based on short chain alcohol and salt was the method for preconcentration, separation and analysis of chloramphenicol (CAM), coupled with high performance liquid chromatography with ultraviolet-visible detector (HPLC-UV). In order to select the suitable ATPS for CAM extraction, different ATPSs were tested and n-propanol/potassium citrate ATPS was chosen for separating and concentrating CAM in the subsequent experiments. The influences of the salt concentration, pH and the volume of n-propanol on the extraction efficiency and partition coefficient of CAM were examined. Response surface methodology was employed to optimize the experimental conditions. Under the optimal conditions, this method has been applied to quantitative determination of CAM in livestock meat samples with limit of detection of 0.48 ng g-1 and limit of quantification of 1.6 ng g-1 with a recovery range of 92.39-104.12%. This ATPS used low cost of organic solvents and supplied a moderate and biocompatible environment, which is suitable for biomolecules.Sociedade Brasileira de Química2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000400021Journal of the Brazilian Chemical Society v.24 n.4 2013reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20130088info:eu-repo/semantics/openAccessLi,YanfangHan,JuanWang,YunMa,JingjingYan,YongshengCao,Lihuaeng2013-05-20T00:00:00Zoai:scielo:S0103-50532013000400021Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-05-20T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC |
title |
Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC |
spellingShingle |
Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC Li,Yanfang HPLC aqueous two-phase system extraction chloramphenicol livestock meat ATPS |
title_short |
Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC |
title_full |
Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC |
title_fullStr |
Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC |
title_full_unstemmed |
Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC |
title_sort |
Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC |
author |
Li,Yanfang |
author_facet |
Li,Yanfang Han,Juan Wang,Yun Ma,Jingjing Yan,Yongsheng Cao,Lihua |
author_role |
author |
author2 |
Han,Juan Wang,Yun Ma,Jingjing Yan,Yongsheng Cao,Lihua |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Li,Yanfang Han,Juan Wang,Yun Ma,Jingjing Yan,Yongsheng Cao,Lihua |
dc.subject.por.fl_str_mv |
HPLC aqueous two-phase system extraction chloramphenicol livestock meat ATPS |
topic |
HPLC aqueous two-phase system extraction chloramphenicol livestock meat ATPS |
description |
An aqueous two-phase system (ATPS) based on short chain alcohol and salt was the method for preconcentration, separation and analysis of chloramphenicol (CAM), coupled with high performance liquid chromatography with ultraviolet-visible detector (HPLC-UV). In order to select the suitable ATPS for CAM extraction, different ATPSs were tested and n-propanol/potassium citrate ATPS was chosen for separating and concentrating CAM in the subsequent experiments. The influences of the salt concentration, pH and the volume of n-propanol on the extraction efficiency and partition coefficient of CAM were examined. Response surface methodology was employed to optimize the experimental conditions. Under the optimal conditions, this method has been applied to quantitative determination of CAM in livestock meat samples with limit of detection of 0.48 ng g-1 and limit of quantification of 1.6 ng g-1 with a recovery range of 92.39-104.12%. This ATPS used low cost of organic solvents and supplied a moderate and biocompatible environment, which is suitable for biomolecules. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000400021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000400021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20130088 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.24 n.4 2013 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318174835834880 |