Quantification of Arsenic, Cadmium, Mercury and Lead in Pineapple: Method Development, Validation and Evaluation of In-House Reference Materials

• Autor(a) principal: Garzón,Diego A.
• Data de Publicação: 2020
• Outros Autores: Ahumada,Diego A., Gamba,Johanna P. Abella, Ágreda,Jesus, Castillo,Elianna
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532020000601296
• topic: pineapple; gravimetric standard addition; toxic elements; ICP-MS; in-house reference material

A method for quantification of arsenic, cadmium, mercury, and lead in pineapple using inductively coupled plasma-mass spectrometry (ICP-MS) was developed, validated, and used to test the homogeneity and stability of in-house reference materials. The method was tested at the regulation levels given by the Food and Agriculture Organization of the United Nations (FAO, 100 µg kg−1). The mineralization conditions of the samples, in a microwave oven, were improved throughout the study of HNO3 and H2O2 concentrations, sample weight, time, and temperatures. The calibration was made by a gravimetric standard addition method with internal standard correction. The validation parameters were accuracy (trueness as recovery, between 97-112%, and precision as repeatability, below 12%), limits of detection (As 14.2, Cd 1.5, Hg 21.9, and Pb 5.4 µg kg−1 measured in pineapple), linearity and selectivity. The in-house material was lyophilized or dried in an oven. The best way to dry it was by the oven process because it has the lowest uncertainty.