Detecção de inibidores de proteases em sementes de Punica granatum
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270 |
Resumo: | The use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They are found in plants, especially in Fabaceae, Poaceae, and Solanaceae families, and show satisfactory use to treat diseases due to their regulatory activities and selective proteolytic degradation of target substrates. This study aimed to obtain protease inhibitors in the seeds of Punica granatum, due its use in traditional medicine. The alcohol-acid solution was used to extract and isolate the protease inhibitors in P. granatum seeds and Glycine max grains. The extract of P. granatum seeds showed similar concentration of protease inhibitors when compared to G. max. A 14 kDa band was detected by electrophoresis on polyacrylamide gel, which was characterized by high-performance liquid chromatography as protease inhibitors. The extract of P. granatum seeds showed a potent trypsin inhibitor activity (EC50 18.2 ng mL-1). This study showed for the first time the extraction, purification, and identification of protease inhibitors in Punica granatum seeds. |
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Detecção de inibidores de proteases em sementes de Punica granatumprotease inhibitorsPunica granatum seedstraditional medicineThe use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They are found in plants, especially in Fabaceae, Poaceae, and Solanaceae families, and show satisfactory use to treat diseases due to their regulatory activities and selective proteolytic degradation of target substrates. This study aimed to obtain protease inhibitors in the seeds of Punica granatum, due its use in traditional medicine. The alcohol-acid solution was used to extract and isolate the protease inhibitors in P. granatum seeds and Glycine max grains. The extract of P. granatum seeds showed similar concentration of protease inhibitors when compared to G. max. A 14 kDa band was detected by electrophoresis on polyacrylamide gel, which was characterized by high-performance liquid chromatography as protease inhibitors. The extract of P. granatum seeds showed a potent trypsin inhibitor activity (EC50 18.2 ng mL-1). This study showed for the first time the extraction, purification, and identification of protease inhibitors in Punica granatum seeds.Sociedade Brasileira de Química2017-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270Química Nova v.40 n.3 2017reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20160189info:eu-repo/semantics/openAccessColares,Lara FrancaSantos Neto,Antero RicardoCordeiro,Isac HenriqueCastro,Sandra Bertelli Ribeiro deVerly,Rodrigo MoreiraAlves,Caio Cesar de SouzaCarli,Alessandra de Paulapor2017-05-05T00:00:00Zoai:scielo:S0100-40422017000300270Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2017-05-05T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Detecção de inibidores de proteases em sementes de Punica granatum |
title |
Detecção de inibidores de proteases em sementes de Punica granatum |
spellingShingle |
Detecção de inibidores de proteases em sementes de Punica granatum Colares,Lara Franca protease inhibitors Punica granatum seeds traditional medicine |
title_short |
Detecção de inibidores de proteases em sementes de Punica granatum |
title_full |
Detecção de inibidores de proteases em sementes de Punica granatum |
title_fullStr |
Detecção de inibidores de proteases em sementes de Punica granatum |
title_full_unstemmed |
Detecção de inibidores de proteases em sementes de Punica granatum |
title_sort |
Detecção de inibidores de proteases em sementes de Punica granatum |
author |
Colares,Lara Franca |
author_facet |
Colares,Lara Franca Santos Neto,Antero Ricardo Cordeiro,Isac Henrique Castro,Sandra Bertelli Ribeiro de Verly,Rodrigo Moreira Alves,Caio Cesar de Souza Carli,Alessandra de Paula |
author_role |
author |
author2 |
Santos Neto,Antero Ricardo Cordeiro,Isac Henrique Castro,Sandra Bertelli Ribeiro de Verly,Rodrigo Moreira Alves,Caio Cesar de Souza Carli,Alessandra de Paula |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Colares,Lara Franca Santos Neto,Antero Ricardo Cordeiro,Isac Henrique Castro,Sandra Bertelli Ribeiro de Verly,Rodrigo Moreira Alves,Caio Cesar de Souza Carli,Alessandra de Paula |
dc.subject.por.fl_str_mv |
protease inhibitors Punica granatum seeds traditional medicine |
topic |
protease inhibitors Punica granatum seeds traditional medicine |
description |
The use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They are found in plants, especially in Fabaceae, Poaceae, and Solanaceae families, and show satisfactory use to treat diseases due to their regulatory activities and selective proteolytic degradation of target substrates. This study aimed to obtain protease inhibitors in the seeds of Punica granatum, due its use in traditional medicine. The alcohol-acid solution was used to extract and isolate the protease inhibitors in P. granatum seeds and Glycine max grains. The extract of P. granatum seeds showed similar concentration of protease inhibitors when compared to G. max. A 14 kDa band was detected by electrophoresis on polyacrylamide gel, which was characterized by high-performance liquid chromatography as protease inhibitors. The extract of P. granatum seeds showed a potent trypsin inhibitor activity (EC50 18.2 ng mL-1). This study showed for the first time the extraction, purification, and identification of protease inhibitors in Punica granatum seeds. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20160189 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.40 n.3 2017 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318118001967104 |