Extração de esterase de fígado suíno (PLE)

Detalhes bibliográficos
Autor(a) principal: Trevisan,Henrique Celso
Data de Publicação: 2006
Outros Autores: Medeiros,João Batista de, Lisboa,Helen Cristina Fávero
Tipo de documento: Relatório
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000400040
Resumo: A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.
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spelling Extração de esterase de fígado suíno (PLE)porcine liver esterasePLEbiocatalysisA simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.Sociedade Brasileira de Química2006-07-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000400040Química Nova v.29 n.4 2006reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422006000400040info:eu-repo/semantics/openAccessTrevisan,Henrique CelsoMedeiros,João Batista deLisboa,Helen Cristina Fáveropor2006-07-31T00:00:00Zoai:scielo:S0100-40422006000400040Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2006-07-31T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Extração de esterase de fígado suíno (PLE)
title Extração de esterase de fígado suíno (PLE)
spellingShingle Extração de esterase de fígado suíno (PLE)
Trevisan,Henrique Celso
porcine liver esterase
PLE
biocatalysis
title_short Extração de esterase de fígado suíno (PLE)
title_full Extração de esterase de fígado suíno (PLE)
title_fullStr Extração de esterase de fígado suíno (PLE)
title_full_unstemmed Extração de esterase de fígado suíno (PLE)
title_sort Extração de esterase de fígado suíno (PLE)
author Trevisan,Henrique Celso
author_facet Trevisan,Henrique Celso
Medeiros,João Batista de
Lisboa,Helen Cristina Fávero
author_role author
author2 Medeiros,João Batista de
Lisboa,Helen Cristina Fávero
author2_role author
author
dc.contributor.author.fl_str_mv Trevisan,Henrique Celso
Medeiros,João Batista de
Lisboa,Helen Cristina Fávero
dc.subject.por.fl_str_mv porcine liver esterase
PLE
biocatalysis
topic porcine liver esterase
PLE
biocatalysis
description A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.
publishDate 2006
dc.date.none.fl_str_mv 2006-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000400040
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000400040
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422006000400040
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.29 n.4 2006
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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