Pig liver esterase (PLE) extraction.

Detalhes bibliográficos
Autor(a) principal: Trevisan, Henrique Celso
Data de Publicação: 2006
Outros Autores: Medeiros, Joao Batista de, Favero Lisboa, Helen Cristina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/195856
Resumo: A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 W of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4 degrees C.
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spelling Pig liver esterase (PLE) extraction.porcine liver esterasePLEbiocatalysisA simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 W of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4 degrees C.Univ Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14800900 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14800900 Araraquara, SP, BrazilSoc Brasileira QuimicaUniversidade Estadual Paulista (Unesp)Trevisan, Henrique CelsoMedeiros, Joao Batista deFavero Lisboa, Helen Cristina2020-12-10T18:05:35Z2020-12-10T18:05:35Z2006-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article865-867Quimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 29, n. 4, p. 865-867, 2006.0100-4042http://hdl.handle.net/11449/195856WOS:000239388400040Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporQuimica Novainfo:eu-repo/semantics/openAccess2021-10-23T12:19:08Zoai:repositorio.unesp.br:11449/195856Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:19:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Pig liver esterase (PLE) extraction.
title Pig liver esterase (PLE) extraction.
spellingShingle Pig liver esterase (PLE) extraction.
Trevisan, Henrique Celso
porcine liver esterase
PLE
biocatalysis
title_short Pig liver esterase (PLE) extraction.
title_full Pig liver esterase (PLE) extraction.
title_fullStr Pig liver esterase (PLE) extraction.
title_full_unstemmed Pig liver esterase (PLE) extraction.
title_sort Pig liver esterase (PLE) extraction.
author Trevisan, Henrique Celso
author_facet Trevisan, Henrique Celso
Medeiros, Joao Batista de
Favero Lisboa, Helen Cristina
author_role author
author2 Medeiros, Joao Batista de
Favero Lisboa, Helen Cristina
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Trevisan, Henrique Celso
Medeiros, Joao Batista de
Favero Lisboa, Helen Cristina
dc.subject.por.fl_str_mv porcine liver esterase
PLE
biocatalysis
topic porcine liver esterase
PLE
biocatalysis
description A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 W of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4 degrees C.
publishDate 2006
dc.date.none.fl_str_mv 2006-07-01
2020-12-10T18:05:35Z
2020-12-10T18:05:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Quimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 29, n. 4, p. 865-867, 2006.
0100-4042
http://hdl.handle.net/11449/195856
WOS:000239388400040
identifier_str_mv Quimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 29, n. 4, p. 865-867, 2006.
0100-4042
WOS:000239388400040
url http://hdl.handle.net/11449/195856
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Quimica Nova
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 865-867
dc.publisher.none.fl_str_mv Soc Brasileira Quimica
publisher.none.fl_str_mv Soc Brasileira Quimica
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803649978491469824

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