ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL

Detalhes bibliográficos
Autor(a) principal: Ficanha,Aline Matuella Moreira
Data de Publicação: 2015
Outros Autores: Nyari,Nádia Ligianara D., Levandoski,Katarine, Mignoni,Marcelo Luis, Dallago,Rogério Marcos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300364
Resumo: The objective of this work was the immobilization of the enzyme Candida antarctica lipase B (CAL B) using the sol-gel method of immobilization and three different initiators of the polymerization reaction: one acid (HCl), one basic (NH4OH) and the other nucleophilic (HBr). Tetraethylorthosilicate was used as the silica precursor. The influence of the additive PEG 1500 on immobilization was assessed. The efficiency of the process was evaluated considering the esterification activity of the xerogels. The immobilization process provided enhanced thermal stability, storage and operational aspects relative to the free enzyme. Storage temperature proved one of the main variables to be considered in the process, with the xerogels stored under refrigeration showing better results in terms of residual activity (nearly 200 days with ≥ 90% residual activity of basic and nucleophilic xerogels) when compared with storage at ambient temperature (nearly 40 days). The results demonstrated the possibility of reuse of derivatives and a greater number of cycles (nine), considering a residual activity of 50%.
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spelling ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GELimmobilizationlipasesol-gelPEG 1500esterificationThe objective of this work was the immobilization of the enzyme Candida antarctica lipase B (CAL B) using the sol-gel method of immobilization and three different initiators of the polymerization reaction: one acid (HCl), one basic (NH4OH) and the other nucleophilic (HBr). Tetraethylorthosilicate was used as the silica precursor. The influence of the additive PEG 1500 on immobilization was assessed. The efficiency of the process was evaluated considering the esterification activity of the xerogels. The immobilization process provided enhanced thermal stability, storage and operational aspects relative to the free enzyme. Storage temperature proved one of the main variables to be considered in the process, with the xerogels stored under refrigeration showing better results in terms of residual activity (nearly 200 days with ≥ 90% residual activity of basic and nucleophilic xerogels) when compared with storage at ambient temperature (nearly 40 days). The results demonstrated the possibility of reuse of derivatives and a greater number of cycles (nine), considering a residual activity of 50%.Sociedade Brasileira de Química2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300364Química Nova v.38 n.3 2015reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0100-4042.20150027info:eu-repo/semantics/openAccessFicanha,Aline Matuella MoreiraNyari,Nádia Ligianara D.Levandoski,KatarineMignoni,Marcelo LuisDallago,Rogério Marcospor2015-05-12T00:00:00Zoai:scielo:S0100-40422015000300364Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2015-05-12T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
title ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
spellingShingle ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
Ficanha,Aline Matuella Moreira
immobilization
lipase
sol-gel
PEG 1500
esterification
title_short ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
title_full ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
title_fullStr ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
title_full_unstemmed ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
title_sort ESTUDO DA IMOBILIZAÇÃO DE LIPASE EM SÍLICA OBTIDA PELA TÉCNICA SOL-GEL
author Ficanha,Aline Matuella Moreira
author_facet Ficanha,Aline Matuella Moreira
Nyari,Nádia Ligianara D.
Levandoski,Katarine
Mignoni,Marcelo Luis
Dallago,Rogério Marcos
author_role author
author2 Nyari,Nádia Ligianara D.
Levandoski,Katarine
Mignoni,Marcelo Luis
Dallago,Rogério Marcos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ficanha,Aline Matuella Moreira
Nyari,Nádia Ligianara D.
Levandoski,Katarine
Mignoni,Marcelo Luis
Dallago,Rogério Marcos
dc.subject.por.fl_str_mv immobilization
lipase
sol-gel
PEG 1500
esterification
topic immobilization
lipase
sol-gel
PEG 1500
esterification
description The objective of this work was the immobilization of the enzyme Candida antarctica lipase B (CAL B) using the sol-gel method of immobilization and three different initiators of the polymerization reaction: one acid (HCl), one basic (NH4OH) and the other nucleophilic (HBr). Tetraethylorthosilicate was used as the silica precursor. The influence of the additive PEG 1500 on immobilization was assessed. The efficiency of the process was evaluated considering the esterification activity of the xerogels. The immobilization process provided enhanced thermal stability, storage and operational aspects relative to the free enzyme. Storage temperature proved one of the main variables to be considered in the process, with the xerogels stored under refrigeration showing better results in terms of residual activity (nearly 200 days with ≥ 90% residual activity of basic and nucleophilic xerogels) when compared with storage at ambient temperature (nearly 40 days). The results demonstrated the possibility of reuse of derivatives and a greater number of cycles (nine), considering a residual activity of 50%.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300364
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300364
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.5935/0100-4042.20150027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.38 n.3 2015
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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