Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas

Detalhes bibliográficos
Autor(a) principal: Nunes,Gilvanda Silva
Data de Publicação: 2005
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000300018
Resumo: Immunoassay techniques provide simple, powerful and inexpensive methods for analysis of environmental contaminants. However, the acceptance of immunoassays is dependent on the clear demonstration of quality and validity compared to more traditional techniques. In this review, primarily, the understanding and the fundamentals of immunoassay methods are given in order to make good use of immunoassays, especially of EIA tests. Special attention is given to the concepts related to the enzyme-linked immunosorbent assay (ELISA) formats, such as inhibition concentration at 50% (IC50), detection limit (LOD), cross-reactivity (CR %). It is also explained why some immunoassays are quantitative methods whereas others can only be used as screening methods. A list of main commercial kits for detection of priority pollutants is given in order to help analysts. Others formats, such as flow-injection immunoassay analysis (FIIA), immunoassay chromatography and immunosensors are also cited.
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spelling Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivasimmunoassaysenvironmental contaminantsmonitoringImmunoassay techniques provide simple, powerful and inexpensive methods for analysis of environmental contaminants. However, the acceptance of immunoassays is dependent on the clear demonstration of quality and validity compared to more traditional techniques. In this review, primarily, the understanding and the fundamentals of immunoassay methods are given in order to make good use of immunoassays, especially of EIA tests. Special attention is given to the concepts related to the enzyme-linked immunosorbent assay (ELISA) formats, such as inhibition concentration at 50% (IC50), detection limit (LOD), cross-reactivity (CR %). It is also explained why some immunoassays are quantitative methods whereas others can only be used as screening methods. A list of main commercial kits for detection of priority pollutants is given in order to help analysts. Others formats, such as flow-injection immunoassay analysis (FIIA), immunoassay chromatography and immunosensors are also cited.Sociedade Brasileira de Química2005-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000300018Química Nova v.28 n.3 2005reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422005000300018info:eu-repo/semantics/openAccessNunes,Gilvanda Silvapor2005-06-14T00:00:00Zoai:scielo:S0100-40422005000300018Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2005-06-14T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas
title Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas
spellingShingle Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas
Nunes,Gilvanda Silva
immunoassays
environmental contaminants
monitoring
title_short Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas
title_full Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas
title_fullStr Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas
title_full_unstemmed Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas
title_sort Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas
author Nunes,Gilvanda Silva
author_facet Nunes,Gilvanda Silva
author_role author
dc.contributor.author.fl_str_mv Nunes,Gilvanda Silva
dc.subject.por.fl_str_mv immunoassays
environmental contaminants
monitoring
topic immunoassays
environmental contaminants
monitoring
description Immunoassay techniques provide simple, powerful and inexpensive methods for analysis of environmental contaminants. However, the acceptance of immunoassays is dependent on the clear demonstration of quality and validity compared to more traditional techniques. In this review, primarily, the understanding and the fundamentals of immunoassay methods are given in order to make good use of immunoassays, especially of EIA tests. Special attention is given to the concepts related to the enzyme-linked immunosorbent assay (ELISA) formats, such as inhibition concentration at 50% (IC50), detection limit (LOD), cross-reactivity (CR %). It is also explained why some immunoassays are quantitative methods whereas others can only be used as screening methods. A list of main commercial kits for detection of priority pollutants is given in order to help analysts. Others formats, such as flow-injection immunoassay analysis (FIIA), immunoassay chromatography and immunosensors are also cited.
publishDate 2005
dc.date.none.fl_str_mv 2005-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000300018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422005000300018
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422005000300018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.28 n.3 2005
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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