Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves

Detalhes bibliográficos
Autor(a) principal: Maciel,Hermelinda Penha Freire
Data de Publicação: 2007
Outros Autores: Gouvêa,Cibele Marli Cação Paiva, Toyama,Marcos, Smolka,Marcus, Marangoni,Sergio, Pastore,Gláucia Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000500003
Resumo: The aim of this work is to obtain, purify and characterize biochemically a peroxidase from Copaifera langsdorffii leaves (COP). COP was obtained by acetone precipitation followed by ion-exchange chromatography. Purification yielded 3.5% of peroxidase with the purification factor of 46.86. The COP optimum pH is 6.0 and the temperature is 35 ºC. COP was stable in the pH range of 4.5 to 9.3 and at temperatures below 50.0 ºC. The apparent Michaelis-Menten constants (Km) for guaiacol and H2O2 were 0.04 mM and 0.39 mM respectively. Enzyme turnover was 0.075 s-1 for guaiacol and 0.28 s-1 for hydrogen peroxide. Copaifera langsdorffii leaves showed to be a rich source of active peroxidase (COP) during the whole year. COP could replace HRP, the most used peroxidase, in analytical determinations and treatment of industrial effluents at low cost.
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spelling Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leavesCopaifera langsdorffiiperoxidasevegetal extractThe aim of this work is to obtain, purify and characterize biochemically a peroxidase from Copaifera langsdorffii leaves (COP). COP was obtained by acetone precipitation followed by ion-exchange chromatography. Purification yielded 3.5% of peroxidase with the purification factor of 46.86. The COP optimum pH is 6.0 and the temperature is 35 ºC. COP was stable in the pH range of 4.5 to 9.3 and at temperatures below 50.0 ºC. The apparent Michaelis-Menten constants (Km) for guaiacol and H2O2 were 0.04 mM and 0.39 mM respectively. Enzyme turnover was 0.075 s-1 for guaiacol and 0.28 s-1 for hydrogen peroxide. Copaifera langsdorffii leaves showed to be a rich source of active peroxidase (COP) during the whole year. COP could replace HRP, the most used peroxidase, in analytical determinations and treatment of industrial effluents at low cost.Sociedade Brasileira de Química2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000500003Química Nova v.30 n.5 2007reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422007000500003info:eu-repo/semantics/openAccessMaciel,Hermelinda Penha FreireGouvêa,Cibele Marli Cação PaivaToyama,MarcosSmolka,MarcusMarangoni,SergioPastore,Gláucia Mariaeng2007-09-28T00:00:00Zoai:scielo:S0100-40422007000500003Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2007-09-28T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
title Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
spellingShingle Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
Maciel,Hermelinda Penha Freire
Copaifera langsdorffii
peroxidase
vegetal extract
title_short Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
title_full Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
title_fullStr Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
title_full_unstemmed Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
title_sort Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
author Maciel,Hermelinda Penha Freire
author_facet Maciel,Hermelinda Penha Freire
Gouvêa,Cibele Marli Cação Paiva
Toyama,Marcos
Smolka,Marcus
Marangoni,Sergio
Pastore,Gláucia Maria
author_role author
author2 Gouvêa,Cibele Marli Cação Paiva
Toyama,Marcos
Smolka,Marcus
Marangoni,Sergio
Pastore,Gláucia Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Maciel,Hermelinda Penha Freire
Gouvêa,Cibele Marli Cação Paiva
Toyama,Marcos
Smolka,Marcus
Marangoni,Sergio
Pastore,Gláucia Maria
dc.subject.por.fl_str_mv Copaifera langsdorffii
peroxidase
vegetal extract
topic Copaifera langsdorffii
peroxidase
vegetal extract
description The aim of this work is to obtain, purify and characterize biochemically a peroxidase from Copaifera langsdorffii leaves (COP). COP was obtained by acetone precipitation followed by ion-exchange chromatography. Purification yielded 3.5% of peroxidase with the purification factor of 46.86. The COP optimum pH is 6.0 and the temperature is 35 ºC. COP was stable in the pH range of 4.5 to 9.3 and at temperatures below 50.0 ºC. The apparent Michaelis-Menten constants (Km) for guaiacol and H2O2 were 0.04 mM and 0.39 mM respectively. Enzyme turnover was 0.075 s-1 for guaiacol and 0.28 s-1 for hydrogen peroxide. Copaifera langsdorffii leaves showed to be a rich source of active peroxidase (COP) during the whole year. COP could replace HRP, the most used peroxidase, in analytical determinations and treatment of industrial effluents at low cost.
publishDate 2007
dc.date.none.fl_str_mv 2007-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000500003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000500003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422007000500003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.30 n.5 2007
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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