Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200005 |
Resumo: | Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306). The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable. |
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Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extendersartificial inseminationcoolingsemen extenderstallionTwo semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306). The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.Sociedade Brasileira de Zootecnia2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200005Revista Brasileira de Zootecnia v.41 n.12 2012reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/S1516-35982012001200005info:eu-repo/semantics/openAccessPugliesi,GuilhermeCarvalho,Giovanni Ribeiro deRates,Daniel MacêdoKer,Pedro GamaMatta,Manuela Pereira daOliveira,Renan Reis deSilva Filho,José Monteiro daeng2012-12-10T00:00:00Zoai:scielo:S1516-35982012001200005Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2012-12-10T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false |
dc.title.none.fl_str_mv |
Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders |
title |
Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders |
spellingShingle |
Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders Pugliesi,Guilherme artificial insemination cooling semen extender stallion |
title_short |
Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders |
title_full |
Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders |
title_fullStr |
Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders |
title_full_unstemmed |
Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders |
title_sort |
Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders |
author |
Pugliesi,Guilherme |
author_facet |
Pugliesi,Guilherme Carvalho,Giovanni Ribeiro de Rates,Daniel Macêdo Ker,Pedro Gama Matta,Manuela Pereira da Oliveira,Renan Reis de Silva Filho,José Monteiro da |
author_role |
author |
author2 |
Carvalho,Giovanni Ribeiro de Rates,Daniel Macêdo Ker,Pedro Gama Matta,Manuela Pereira da Oliveira,Renan Reis de Silva Filho,José Monteiro da |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pugliesi,Guilherme Carvalho,Giovanni Ribeiro de Rates,Daniel Macêdo Ker,Pedro Gama Matta,Manuela Pereira da Oliveira,Renan Reis de Silva Filho,José Monteiro da |
dc.subject.por.fl_str_mv |
artificial insemination cooling semen extender stallion |
topic |
artificial insemination cooling semen extender stallion |
description |
Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306). The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-35982012001200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
Revista Brasileira de Zootecnia v.41 n.12 2012 reponame:Revista Brasileira de Zootecnia (Online) instname:Sociedade Brasileira de Zootecnia (SBZ) instacron:SBZ |
instname_str |
Sociedade Brasileira de Zootecnia (SBZ) |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Revista Brasileira de Zootecnia (Online) |
collection |
Revista Brasileira de Zootecnia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ) |
repository.mail.fl_str_mv |
||bz@sbz.org.br|| secretariarbz@sbz.org.br |
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1750318150455394304 |