Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2012.05.011 http://hdl.handle.net/11449/14560 |
Resumo: | One of the main factors known to influence quality and fertility of bovine cryopreserved semen is the extender used. In this matter, a great worldwide interest has been directed to the development of chemically defined media, free of animal origin products. The objective of the present study was to compare the efficacy of three bovine semen extenders: Iris-fructose (IRIS, control with 20% egg yolk), Botu-Bov (R) (BB; 20% egg yolk), and Botu-Bov (R)-soy lecithin (BB-L; 1% soy lecithin). Towards this aim, post-thaw computer assisted sperm analysis (CASA), sperm membrane and acrosome integrity were evaluated (Experiment 1). Additionally, cryopreserved samples were used in a fixed time artificial insemination program aiming to evaluate in vivo fertility (pregnancy per insemination-P/AI; Experiment 2). Despite the higher straightness and linearity found for samples cryopreserved in BB and BB-L when compared to those cryopreserved in IRIS, egg yolk based extenders provided higher total and progressive motilities, percentage of rapid sperms and intact membrane cells (P < 0.05). Furthermore, P/IA was higher in samples cryopreserved in egg yolk based extenders when compared to soy lecithin [TRIS=59.26(a) (64/108), BB=62.37(a) (58/93), and BB-L=36.45(b) (35/96)]. Although soy lecithin represents an alternative for the development of chemically defined extenders with decreased risk of biological contamination, egg yolk based extenders are more efficient on the preservation of bovine post-thaw sperm viability and fertility. (C) 2012 Elsevier B.V. All rights reserved. |
id |
UNSP_0f495a3d5a0c0222200e500779a938ee |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/14560 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extendersBull semenSemen extenderEgg yolkSoy lecithinCryopreservationArtificial inseminationOne of the main factors known to influence quality and fertility of bovine cryopreserved semen is the extender used. In this matter, a great worldwide interest has been directed to the development of chemically defined media, free of animal origin products. The objective of the present study was to compare the efficacy of three bovine semen extenders: Iris-fructose (IRIS, control with 20% egg yolk), Botu-Bov (R) (BB; 20% egg yolk), and Botu-Bov (R)-soy lecithin (BB-L; 1% soy lecithin). Towards this aim, post-thaw computer assisted sperm analysis (CASA), sperm membrane and acrosome integrity were evaluated (Experiment 1). Additionally, cryopreserved samples were used in a fixed time artificial insemination program aiming to evaluate in vivo fertility (pregnancy per insemination-P/AI; Experiment 2). Despite the higher straightness and linearity found for samples cryopreserved in BB and BB-L when compared to those cryopreserved in IRIS, egg yolk based extenders provided higher total and progressive motilities, percentage of rapid sperms and intact membrane cells (P < 0.05). Furthermore, P/IA was higher in samples cryopreserved in egg yolk based extenders when compared to soy lecithin [TRIS=59.26(a) (64/108), BB=62.37(a) (58/93), and BB-L=36.45(b) (35/96)]. Although soy lecithin represents an alternative for the development of chemically defined extenders with decreased risk of biological contamination, egg yolk based extenders are more efficient on the preservation of bovine post-thaw sperm viability and fertility. (C) 2012 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ, Dept Anim Reprod & Vet Radiol, Botucatu, SP, BrazilUniv Santo Amaro, UNISA, Sch Vet Med, São Paulo, BrazilUniv São Paulo, Dept Anim Reprod, São Paulo, BrazilSão Paulo State Univ, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilSão Paulo State Univ, Dept Anim Reprod & Vet Radiol, Botucatu, SP, BrazilSão Paulo State Univ, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilFAPESP: 06/61153-5Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ Santo AmaroUniversidade de São Paulo (USP)Crespilho, A. M.Sa Filho, M. F.Dell'aqua Junior, José Antônio [UNESP]Nichi, M.Monteiro, G. A. [UNESP]Avanzi, B. R. [UNESP]Martins, A. [UNESP]Papa, Frederico Ozanam [UNESP]2013-09-30T18:25:28Z2014-05-20T13:41:58Z2013-09-30T18:25:28Z2014-05-20T13:41:58Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article01/junapplication/pdfhttp://dx.doi.org/10.1016/j.livsci.2012.05.011Livestock Science. Amsterdam: Elsevier B.V., v. 149, n. 1-2, p. 1-6, 2012.1871-1413http://hdl.handle.net/11449/1456010.1016/j.livsci.2012.05.011WOS:000309793700001WOS000309793700001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science1.2040,730info:eu-repo/semantics/openAccess2024-09-09T14:05:33Zoai:repositorio.unesp.br:11449/14560Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:05:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders |
title |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders |
spellingShingle |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders Crespilho, A. M. Bull semen Semen extender Egg yolk Soy lecithin Cryopreservation Artificial insemination |
title_short |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders |
title_full |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders |
title_fullStr |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders |
title_full_unstemmed |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders |
title_sort |
Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders |
author |
Crespilho, A. M. |
author_facet |
Crespilho, A. M. Sa Filho, M. F. Dell'aqua Junior, José Antônio [UNESP] Nichi, M. Monteiro, G. A. [UNESP] Avanzi, B. R. [UNESP] Martins, A. [UNESP] Papa, Frederico Ozanam [UNESP] |
author_role |
author |
author2 |
Sa Filho, M. F. Dell'aqua Junior, José Antônio [UNESP] Nichi, M. Monteiro, G. A. [UNESP] Avanzi, B. R. [UNESP] Martins, A. [UNESP] Papa, Frederico Ozanam [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Santo Amaro Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Crespilho, A. M. Sa Filho, M. F. Dell'aqua Junior, José Antônio [UNESP] Nichi, M. Monteiro, G. A. [UNESP] Avanzi, B. R. [UNESP] Martins, A. [UNESP] Papa, Frederico Ozanam [UNESP] |
dc.subject.por.fl_str_mv |
Bull semen Semen extender Egg yolk Soy lecithin Cryopreservation Artificial insemination |
topic |
Bull semen Semen extender Egg yolk Soy lecithin Cryopreservation Artificial insemination |
description |
One of the main factors known to influence quality and fertility of bovine cryopreserved semen is the extender used. In this matter, a great worldwide interest has been directed to the development of chemically defined media, free of animal origin products. The objective of the present study was to compare the efficacy of three bovine semen extenders: Iris-fructose (IRIS, control with 20% egg yolk), Botu-Bov (R) (BB; 20% egg yolk), and Botu-Bov (R)-soy lecithin (BB-L; 1% soy lecithin). Towards this aim, post-thaw computer assisted sperm analysis (CASA), sperm membrane and acrosome integrity were evaluated (Experiment 1). Additionally, cryopreserved samples were used in a fixed time artificial insemination program aiming to evaluate in vivo fertility (pregnancy per insemination-P/AI; Experiment 2). Despite the higher straightness and linearity found for samples cryopreserved in BB and BB-L when compared to those cryopreserved in IRIS, egg yolk based extenders provided higher total and progressive motilities, percentage of rapid sperms and intact membrane cells (P < 0.05). Furthermore, P/IA was higher in samples cryopreserved in egg yolk based extenders when compared to soy lecithin [TRIS=59.26(a) (64/108), BB=62.37(a) (58/93), and BB-L=36.45(b) (35/96)]. Although soy lecithin represents an alternative for the development of chemically defined extenders with decreased risk of biological contamination, egg yolk based extenders are more efficient on the preservation of bovine post-thaw sperm viability and fertility. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 2013-09-30T18:25:28Z 2013-09-30T18:25:28Z 2014-05-20T13:41:58Z 2014-05-20T13:41:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2012.05.011 Livestock Science. Amsterdam: Elsevier B.V., v. 149, n. 1-2, p. 1-6, 2012. 1871-1413 http://hdl.handle.net/11449/14560 10.1016/j.livsci.2012.05.011 WOS:000309793700001 WOS000309793700001.pdf |
url |
http://dx.doi.org/10.1016/j.livsci.2012.05.011 http://hdl.handle.net/11449/14560 |
identifier_str_mv |
Livestock Science. Amsterdam: Elsevier B.V., v. 149, n. 1-2, p. 1-6, 2012. 1871-1413 10.1016/j.livsci.2012.05.011 WOS:000309793700001 WOS000309793700001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science 1.204 0,730 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
01/jun application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546595217571840 |