Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders

Detalhes bibliográficos
Autor(a) principal: Crespilho, A. M.
Data de Publicação: 2012
Outros Autores: Sa Filho, M. F., Dell'aqua Junior, José Antônio [UNESP], Nichi, M., Monteiro, G. A. [UNESP], Avanzi, B. R. [UNESP], Martins, A. [UNESP], Papa, Frederico Ozanam [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.livsci.2012.05.011
http://hdl.handle.net/11449/14560
Resumo: One of the main factors known to influence quality and fertility of bovine cryopreserved semen is the extender used. In this matter, a great worldwide interest has been directed to the development of chemically defined media, free of animal origin products. The objective of the present study was to compare the efficacy of three bovine semen extenders: Iris-fructose (IRIS, control with 20% egg yolk), Botu-Bov (R) (BB; 20% egg yolk), and Botu-Bov (R)-soy lecithin (BB-L; 1% soy lecithin). Towards this aim, post-thaw computer assisted sperm analysis (CASA), sperm membrane and acrosome integrity were evaluated (Experiment 1). Additionally, cryopreserved samples were used in a fixed time artificial insemination program aiming to evaluate in vivo fertility (pregnancy per insemination-P/AI; Experiment 2). Despite the higher straightness and linearity found for samples cryopreserved in BB and BB-L when compared to those cryopreserved in IRIS, egg yolk based extenders provided higher total and progressive motilities, percentage of rapid sperms and intact membrane cells (P < 0.05). Furthermore, P/IA was higher in samples cryopreserved in egg yolk based extenders when compared to soy lecithin [TRIS=59.26(a) (64/108), BB=62.37(a) (58/93), and BB-L=36.45(b) (35/96)]. Although soy lecithin represents an alternative for the development of chemically defined extenders with decreased risk of biological contamination, egg yolk based extenders are more efficient on the preservation of bovine post-thaw sperm viability and fertility. (C) 2012 Elsevier B.V. All rights reserved.
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spelling Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extendersBull semenSemen extenderEgg yolkSoy lecithinCryopreservationArtificial inseminationOne of the main factors known to influence quality and fertility of bovine cryopreserved semen is the extender used. In this matter, a great worldwide interest has been directed to the development of chemically defined media, free of animal origin products. The objective of the present study was to compare the efficacy of three bovine semen extenders: Iris-fructose (IRIS, control with 20% egg yolk), Botu-Bov (R) (BB; 20% egg yolk), and Botu-Bov (R)-soy lecithin (BB-L; 1% soy lecithin). Towards this aim, post-thaw computer assisted sperm analysis (CASA), sperm membrane and acrosome integrity were evaluated (Experiment 1). Additionally, cryopreserved samples were used in a fixed time artificial insemination program aiming to evaluate in vivo fertility (pregnancy per insemination-P/AI; Experiment 2). Despite the higher straightness and linearity found for samples cryopreserved in BB and BB-L when compared to those cryopreserved in IRIS, egg yolk based extenders provided higher total and progressive motilities, percentage of rapid sperms and intact membrane cells (P < 0.05). Furthermore, P/IA was higher in samples cryopreserved in egg yolk based extenders when compared to soy lecithin [TRIS=59.26(a) (64/108), BB=62.37(a) (58/93), and BB-L=36.45(b) (35/96)]. Although soy lecithin represents an alternative for the development of chemically defined extenders with decreased risk of biological contamination, egg yolk based extenders are more efficient on the preservation of bovine post-thaw sperm viability and fertility. (C) 2012 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ, Dept Anim Reprod & Vet Radiol, Botucatu, SP, BrazilUniv Santo Amaro, UNISA, Sch Vet Med, São Paulo, BrazilUniv São Paulo, Dept Anim Reprod, São Paulo, BrazilSão Paulo State Univ, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilSão Paulo State Univ, Dept Anim Reprod & Vet Radiol, Botucatu, SP, BrazilSão Paulo State Univ, Dept Clin Surg & Anim Reprod, Aracatuba, SP, BrazilFAPESP: 06/61153-5Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ Santo AmaroUniversidade de São Paulo (USP)Crespilho, A. M.Sa Filho, M. F.Dell'aqua Junior, José Antônio [UNESP]Nichi, M.Monteiro, G. A. [UNESP]Avanzi, B. R. [UNESP]Martins, A. [UNESP]Papa, Frederico Ozanam [UNESP]2013-09-30T18:25:28Z2014-05-20T13:41:58Z2013-09-30T18:25:28Z2014-05-20T13:41:58Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article01/junapplication/pdfhttp://dx.doi.org/10.1016/j.livsci.2012.05.011Livestock Science. Amsterdam: Elsevier B.V., v. 149, n. 1-2, p. 1-6, 2012.1871-1413http://hdl.handle.net/11449/1456010.1016/j.livsci.2012.05.011WOS:000309793700001WOS000309793700001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science1.2040,730info:eu-repo/semantics/openAccess2024-09-09T14:05:33Zoai:repositorio.unesp.br:11449/14560Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:05:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
title Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
spellingShingle Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
Crespilho, A. M.
Bull semen
Semen extender
Egg yolk
Soy lecithin
Cryopreservation
Artificial insemination
title_short Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
title_full Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
title_fullStr Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
title_full_unstemmed Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
title_sort Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders
author Crespilho, A. M.
author_facet Crespilho, A. M.
Sa Filho, M. F.
Dell'aqua Junior, José Antônio [UNESP]
Nichi, M.
Monteiro, G. A. [UNESP]
Avanzi, B. R. [UNESP]
Martins, A. [UNESP]
Papa, Frederico Ozanam [UNESP]
author_role author
author2 Sa Filho, M. F.
Dell'aqua Junior, José Antônio [UNESP]
Nichi, M.
Monteiro, G. A. [UNESP]
Avanzi, B. R. [UNESP]
Martins, A. [UNESP]
Papa, Frederico Ozanam [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Santo Amaro
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Crespilho, A. M.
Sa Filho, M. F.
Dell'aqua Junior, José Antônio [UNESP]
Nichi, M.
Monteiro, G. A. [UNESP]
Avanzi, B. R. [UNESP]
Martins, A. [UNESP]
Papa, Frederico Ozanam [UNESP]
dc.subject.por.fl_str_mv Bull semen
Semen extender
Egg yolk
Soy lecithin
Cryopreservation
Artificial insemination
topic Bull semen
Semen extender
Egg yolk
Soy lecithin
Cryopreservation
Artificial insemination
description One of the main factors known to influence quality and fertility of bovine cryopreserved semen is the extender used. In this matter, a great worldwide interest has been directed to the development of chemically defined media, free of animal origin products. The objective of the present study was to compare the efficacy of three bovine semen extenders: Iris-fructose (IRIS, control with 20% egg yolk), Botu-Bov (R) (BB; 20% egg yolk), and Botu-Bov (R)-soy lecithin (BB-L; 1% soy lecithin). Towards this aim, post-thaw computer assisted sperm analysis (CASA), sperm membrane and acrosome integrity were evaluated (Experiment 1). Additionally, cryopreserved samples were used in a fixed time artificial insemination program aiming to evaluate in vivo fertility (pregnancy per insemination-P/AI; Experiment 2). Despite the higher straightness and linearity found for samples cryopreserved in BB and BB-L when compared to those cryopreserved in IRIS, egg yolk based extenders provided higher total and progressive motilities, percentage of rapid sperms and intact membrane cells (P < 0.05). Furthermore, P/IA was higher in samples cryopreserved in egg yolk based extenders when compared to soy lecithin [TRIS=59.26(a) (64/108), BB=62.37(a) (58/93), and BB-L=36.45(b) (35/96)]. Although soy lecithin represents an alternative for the development of chemically defined extenders with decreased risk of biological contamination, egg yolk based extenders are more efficient on the preservation of bovine post-thaw sperm viability and fertility. (C) 2012 Elsevier B.V. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-01
2013-09-30T18:25:28Z
2013-09-30T18:25:28Z
2014-05-20T13:41:58Z
2014-05-20T13:41:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.livsci.2012.05.011
Livestock Science. Amsterdam: Elsevier B.V., v. 149, n. 1-2, p. 1-6, 2012.
1871-1413
http://hdl.handle.net/11449/14560
10.1016/j.livsci.2012.05.011
WOS:000309793700001
WOS000309793700001.pdf
url http://dx.doi.org/10.1016/j.livsci.2012.05.011
http://hdl.handle.net/11449/14560
identifier_str_mv Livestock Science. Amsterdam: Elsevier B.V., v. 149, n. 1-2, p. 1-6, 2012.
1871-1413
10.1016/j.livsci.2012.05.011
WOS:000309793700001
WOS000309793700001.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Livestock Science
1.204
0,730
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 01/jun
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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