Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders

Detalhes bibliográficos
Autor(a) principal: Pugliesi, Guilherme
Data de Publicação: 2012
Outros Autores: Carvalho, Giovanni Ribeiro de, Rates, Daniel Macêdo, Ker, Pedro Gama, Matta, Manuela Pereira da, Oliveira, Renan Reis de, Silva Filho, José Monteiro da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1516-35982012001200005
http://www.locus.ufv.br/handle/123456789/14486
Resumo: Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306). The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.
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spelling Pugliesi, GuilhermeCarvalho, Giovanni Ribeiro deRates, Daniel MacêdoKer, Pedro GamaMatta, Manuela Pereira daOliveira, Renan Reis deSilva Filho, José Monteiro da2017-12-06T16:09:21Z2017-12-06T16:09:21Z2012-08-031806-9290http://dx.doi.org/10.1590/S1516-35982012001200005http://www.locus.ufv.br/handle/123456789/14486Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306). The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.engRevista Brasileira de Zootecniav. 41, n. 12, p. 2411-2417, Dec. 2012Artificial inseminationCoolingSemen extenderStallionViability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extendersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALv41n12a05.pdfv41n12a05.pdftexto completoapplication/pdf274944https://locus.ufv.br//bitstream/123456789/14486/1/v41n12a05.pdf4aa687164a387854c3f1bbed78233f42MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/14486/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILv41n12a05.pdf.jpgv41n12a05.pdf.jpgIM Thumbnailimage/jpeg4754https://locus.ufv.br//bitstream/123456789/14486/3/v41n12a05.pdf.jpg04fd33777eafcfdcd2869a684d3e66d7MD53123456789/144862017-12-06 22:00:52.217oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-12-07T01:00:52LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
title Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
spellingShingle Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
Pugliesi, Guilherme
Artificial insemination
Cooling
Semen extender
Stallion
title_short Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
title_full Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
title_fullStr Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
title_full_unstemmed Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
title_sort Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
author Pugliesi, Guilherme
author_facet Pugliesi, Guilherme
Carvalho, Giovanni Ribeiro de
Rates, Daniel Macêdo
Ker, Pedro Gama
Matta, Manuela Pereira da
Oliveira, Renan Reis de
Silva Filho, José Monteiro da
author_role author
author2 Carvalho, Giovanni Ribeiro de
Rates, Daniel Macêdo
Ker, Pedro Gama
Matta, Manuela Pereira da
Oliveira, Renan Reis de
Silva Filho, José Monteiro da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pugliesi, Guilherme
Carvalho, Giovanni Ribeiro de
Rates, Daniel Macêdo
Ker, Pedro Gama
Matta, Manuela Pereira da
Oliveira, Renan Reis de
Silva Filho, José Monteiro da
dc.subject.pt-BR.fl_str_mv Artificial insemination
Cooling
Semen extender
Stallion
topic Artificial insemination
Cooling
Semen extender
Stallion
description Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23 ejaculates from two stallions were split-diluted with Kenney extender and Foote extender. The sperm concentration in each extender was adjusted to 500 million viable sperms per insemination dose. Semen was cooled to 6-8 ºC and stored for 24 hours. Seventy-four cycles of crossbred mares were inseminated with either semen diluted in Kenney extender or semen diluted in Foote extender. The pregnancy rate was higher from semen diluted in Kenney extender than that from semen in Foote extender (0.553 vs. 0.306). The Kenney extender is effective in preserving the motility, vigor and fertility of stallion semen after 24 hours of cold storage, whereas the Foote extender is not acceptable.
publishDate 2012
dc.date.issued.fl_str_mv 2012-08-03
dc.date.accessioned.fl_str_mv 2017-12-06T16:09:21Z
dc.date.available.fl_str_mv 2017-12-06T16:09:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-35982012001200005
http://www.locus.ufv.br/handle/123456789/14486
dc.identifier.issn.none.fl_str_mv 1806-9290
identifier_str_mv 1806-9290
url http://dx.doi.org/10.1590/S1516-35982012001200005
http://www.locus.ufv.br/handle/123456789/14486
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 41, n. 12, p. 2411-2417, Dec. 2012
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dc.publisher.none.fl_str_mv Revista Brasileira de Zootecnia
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