Fitas comestíveis de banana como veículos para probióticos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/15082 |
Resumo: | Considering the growing demand for products that simultaneously meet the requirements of healthiness, good sensory acceptance and convenience, snacks based on fruits or vegetables have been a strong market trend. Another trend is that of food containing probiotics. However, most probiotic foods available on the market are dairy products. With a large number of people with restrictions to the consumption of dairy products and even to animal-based products in general, there is a growing need to introduce non-dairy probiotic foods to the market. The objective of this project was to produce a probiotic banana leather. The fruit leathers were made from banana puree and a film forming matrix. Two matrices were compared – a digestible one (starch) and a non-digestible one (bacterial cellulose - BC), and two probiotic strains (one sporogenic and one non-sporogenic - Bacillus coagulans BC4 and Lactobacillus acidophilus LA-3, respectively), in addition to the presence of a plasticizer and prebiotic agent (yacon syrup). The different formulations were evaluated in terms of their physical properties and viability of the strains after oven drying at 50 °C, during storage at 25 °C and along the simulated passage through the gastrointestinal tract. The leathers presented tensile strength values between 1 and 3 MPa, the ones with BC showing significant higher strengths (p < 0.05), and the presence of probiotics significantly decreasing the tensile strength values. The shear force was also affected by the matrix (being higher for the BC-based leathers) and by the presence of L. acidophilus (which significantly decreased the shear force values). After the dehydration process, the viability of B. coagulans was reduced in about 1 log cycle when compared to that of the film-forming dispersion, showing about 7.6 log cfu g-1, while L. acidophilus did not show enough viability for counting (< 3 log cfu g-1). The viability of B. coagulans remained constant up to 187 days of storage at 25 °C. During the simulated passage through gastrointestinal tract, B. coagulans BC4 resisted well to the pH and enzimatic conditions of the stomach and duodenum, BC- and starch-based leathers released, respectively, 7.45 ± 0.12 e 7.77 ± 0.33 log cfu g-1 in the small intestine (without significant differences between matrices). Therefore, banana leathers can be promising non-dairy foods as carriers of B. coagulans BC4, however, L. acidophilus LA-3 could not be considered a suitable strain for the proposed process. It is recommended that a sensory analysis be carried out in the future with the different formulations of banana leathers to understand how differences in the texture can influence the product acceptance. |
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Niro, Carolina MadazioAzeredo, Henriette Monteiro Cordeiro dehttp://lattes.cnpq.br/8516223928904348http://lattes.cnpq.br/494649594516416930a1acc4-a481-4f0d-8fed-119c8fcd45d52021-11-08T15:04:43Z2021-11-08T15:04:43Z2021-08-18NIRO, Carolina Madazio. Fitas comestíveis de banana como veículos para probióticos. 2021. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15082.https://repositorio.ufscar.br/handle/ufscar/15082Considering the growing demand for products that simultaneously meet the requirements of healthiness, good sensory acceptance and convenience, snacks based on fruits or vegetables have been a strong market trend. Another trend is that of food containing probiotics. However, most probiotic foods available on the market are dairy products. With a large number of people with restrictions to the consumption of dairy products and even to animal-based products in general, there is a growing need to introduce non-dairy probiotic foods to the market. The objective of this project was to produce a probiotic banana leather. The fruit leathers were made from banana puree and a film forming matrix. Two matrices were compared – a digestible one (starch) and a non-digestible one (bacterial cellulose - BC), and two probiotic strains (one sporogenic and one non-sporogenic - Bacillus coagulans BC4 and Lactobacillus acidophilus LA-3, respectively), in addition to the presence of a plasticizer and prebiotic agent (yacon syrup). The different formulations were evaluated in terms of their physical properties and viability of the strains after oven drying at 50 °C, during storage at 25 °C and along the simulated passage through the gastrointestinal tract. The leathers presented tensile strength values between 1 and 3 MPa, the ones with BC showing significant higher strengths (p < 0.05), and the presence of probiotics significantly decreasing the tensile strength values. The shear force was also affected by the matrix (being higher for the BC-based leathers) and by the presence of L. acidophilus (which significantly decreased the shear force values). After the dehydration process, the viability of B. coagulans was reduced in about 1 log cycle when compared to that of the film-forming dispersion, showing about 7.6 log cfu g-1, while L. acidophilus did not show enough viability for counting (< 3 log cfu g-1). The viability of B. coagulans remained constant up to 187 days of storage at 25 °C. During the simulated passage through gastrointestinal tract, B. coagulans BC4 resisted well to the pH and enzimatic conditions of the stomach and duodenum, BC- and starch-based leathers released, respectively, 7.45 ± 0.12 e 7.77 ± 0.33 log cfu g-1 in the small intestine (without significant differences between matrices). Therefore, banana leathers can be promising non-dairy foods as carriers of B. coagulans BC4, however, L. acidophilus LA-3 could not be considered a suitable strain for the proposed process. It is recommended that a sensory analysis be carried out in the future with the different formulations of banana leathers to understand how differences in the texture can influence the product acceptance.Considerando a grande demanda por produtos que atendam simultaneamente aos requisitos de saudabilidade, boa aceitação sensorial e conveniência, snacks à base de frutas ou hortaliças têm se apresentado como uma forte tendência no mercado. Outra tendência são alimentos contendo probióticos. Porém, a grande maioria de alimentos probióticos disponíveis no mercado atualmente são lácteos. Com uma grande quantidade de pessoas com restrições ao consumo de laticínios e mesmo de produtos de origem animal no geral, cresce a necessidade de introduzir no mercado alimentos probióticos não-lácteos. O objetivo do trabalho foi desenvolver uma fita de banana probiótica. As fitas foram formuladas a partir de polpa de banana e uma matriz filmogênica. Foram testadas duas matrizes - uma digerível (amido) e uma não-digerível (celulose bacteriana - CB) - e duas cepas probióticas (esporogênica e não-esporogênica – Bacillus coagulans BC4 e Lactobacillus acidophilus LA-3, respectivamente), além da presença de um agente plastificante (xarope de yacon). As diferentes formulações foram avaliadas em termos de suas propriedades físicas e viabilidade das cepas após o processo de secagem em estufa a 50 °C, durante estocagem a 25 °C e ao longo da passagem simulada pelo trato gastrointestinal. As fitas apresentaram valores de resistência à tração entre 1 e 3 MPa, as fitas feitas com CB apresentaram resistência significativamente (p < 0,05) maior que as fitas feitas com amido, e a presença de probióticos diminuiu significativamente a resistência à tração. A força de cisalhamento também foi afetada pela matriz (sendo maior para as fitas de CB) e pela presença de L. acidophilus (que diminuiu a força de cisalhamento). Após a secagem, a viabilidade de B. coagulans reduziu em cerca de 1 ciclo log em relação à dispersão filmogênica, apresentando cerca de 7,6 log UFC g-1, enquanto L. acidophilus não apresentou viabilidade suficiente para contagem (< 3 log UFC g-1). A viabilidade de B. coagulans permaneceu constante até 187 dias de estocagem em 25 °C. Durante a passagem simulada pelo trato gastrointestinal, as fitas contendo B. coagulans BC4 foram resistentes às condições de pH e enzimáticas do estômago e duodeno, liberando 7,45 ± 0,12 e 7,77 ± 0,33 log UFC g-1 no intestino para as fitas à base de CB e amido, respectivamente (sem diferença significativa para as matrizes). Portanto, fitas de banana podem ser alimentos não-lácteos promissores como carreadores de B. coagulans BC4, porém, L. acidophilus LA-3 não poderia ser considerada uma cepa adequada para o processo proposto. Indica-se que seja feita futuramente uma análise sensorial com as diferentes formulações de fitas de banana para entender como as diferenças na textura das fitas podem influenciar a aceitação do produto.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES: 88882.426523/2019-01porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Biotecnologia - PPGBiotecUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessFitas de frutasProbióticosPolissacarídeosCelulose bacterianaSnacks saudáveisFruit leathersProbioticsPolysaccharidesBacterial celluloseHealthy snacksCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS::MICROBIOLOGIA DE ALIMENTOSCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOSCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOSFitas comestíveis de banana como veículos para probióticosBanana leathers as probiotic carriersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6006000bdfe530-ac00-4c9a-9a2f-a06e4dd13055reponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdfDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdfDissertação de mestradoapplication/pdf2304592https://repositorio.ufscar.br/bitstream/ufscar/15082/1/Disserta%c3%a7%c3%a3o%20de%20Mestrado%20-%20PPGBiotec%20-%20Carolina%20Madazio%20Niro%20-%20Vers%c3%a3o%20Final.pdfc9f2ee32734abf1d0735465df705881fMD51Carta Comprovante.pdfCarta Comprovante.pdfCarta Comprovanteapplication/pdf694988https://repositorio.ufscar.br/bitstream/ufscar/15082/2/Carta%20Comprovante.pdff1a8ec82738c7f5c9e4113b1661c9a34MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/15082/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdf.txtDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdf.txtExtracted texttext/plain155893https://repositorio.ufscar.br/bitstream/ufscar/15082/4/Disserta%c3%a7%c3%a3o%20de%20Mestrado%20-%20PPGBiotec%20-%20Carolina%20Madazio%20Niro%20-%20Vers%c3%a3o%20Final.pdf.txt6cf5c85f7aee56ddf4c74e62942c2fa7MD54Carta Comprovante.pdf.txtCarta Comprovante.pdf.txtExtracted texttext/plain1198https://repositorio.ufscar.br/bitstream/ufscar/15082/6/Carta%20Comprovante.pdf.txt06db3f35d9e8528ef8b564cfd7debea0MD56THUMBNAILDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdf.jpgDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdf.jpgIM Thumbnailimage/jpeg10042https://repositorio.ufscar.br/bitstream/ufscar/15082/5/Disserta%c3%a7%c3%a3o%20de%20Mestrado%20-%20PPGBiotec%20-%20Carolina%20Madazio%20Niro%20-%20Vers%c3%a3o%20Final.pdf.jpgb1dc9f9f20af3c497e654ceb14e88b5eMD55Carta Comprovante.pdf.jpgCarta Comprovante.pdf.jpgIM Thumbnailimage/jpeg6319https://repositorio.ufscar.br/bitstream/ufscar/15082/7/Carta%20Comprovante.pdf.jpg670bf13d485e18d126ea2aefb464ce50MD57ufscar/150822023-09-18 18:32:19.95oai:repositorio.ufscar.br:ufscar/15082Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:19Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Fitas comestíveis de banana como veículos para probióticos |
dc.title.alternative.eng.fl_str_mv |
Banana leathers as probiotic carriers |
title |
Fitas comestíveis de banana como veículos para probióticos |
spellingShingle |
Fitas comestíveis de banana como veículos para probióticos Niro, Carolina Madazio Fitas de frutas Probióticos Polissacarídeos Celulose bacteriana Snacks saudáveis Fruit leathers Probiotics Polysaccharides Bacterial cellulose Healthy snacks CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS::MICROBIOLOGIA DE ALIMENTOS CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
title_short |
Fitas comestíveis de banana como veículos para probióticos |
title_full |
Fitas comestíveis de banana como veículos para probióticos |
title_fullStr |
Fitas comestíveis de banana como veículos para probióticos |
title_full_unstemmed |
Fitas comestíveis de banana como veículos para probióticos |
title_sort |
Fitas comestíveis de banana como veículos para probióticos |
author |
Niro, Carolina Madazio |
author_facet |
Niro, Carolina Madazio |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/4946495945164169 |
dc.contributor.author.fl_str_mv |
Niro, Carolina Madazio |
dc.contributor.advisor1.fl_str_mv |
Azeredo, Henriette Monteiro Cordeiro de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8516223928904348 |
dc.contributor.authorID.fl_str_mv |
30a1acc4-a481-4f0d-8fed-119c8fcd45d5 |
contributor_str_mv |
Azeredo, Henriette Monteiro Cordeiro de |
dc.subject.por.fl_str_mv |
Fitas de frutas Probióticos Polissacarídeos Celulose bacteriana Snacks saudáveis |
topic |
Fitas de frutas Probióticos Polissacarídeos Celulose bacteriana Snacks saudáveis Fruit leathers Probiotics Polysaccharides Bacterial cellulose Healthy snacks CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS::MICROBIOLOGIA DE ALIMENTOS CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Fruit leathers Probiotics Polysaccharides Bacterial cellulose Healthy snacks |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS::MICROBIOLOGIA DE ALIMENTOS CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS |
description |
Considering the growing demand for products that simultaneously meet the requirements of healthiness, good sensory acceptance and convenience, snacks based on fruits or vegetables have been a strong market trend. Another trend is that of food containing probiotics. However, most probiotic foods available on the market are dairy products. With a large number of people with restrictions to the consumption of dairy products and even to animal-based products in general, there is a growing need to introduce non-dairy probiotic foods to the market. The objective of this project was to produce a probiotic banana leather. The fruit leathers were made from banana puree and a film forming matrix. Two matrices were compared – a digestible one (starch) and a non-digestible one (bacterial cellulose - BC), and two probiotic strains (one sporogenic and one non-sporogenic - Bacillus coagulans BC4 and Lactobacillus acidophilus LA-3, respectively), in addition to the presence of a plasticizer and prebiotic agent (yacon syrup). The different formulations were evaluated in terms of their physical properties and viability of the strains after oven drying at 50 °C, during storage at 25 °C and along the simulated passage through the gastrointestinal tract. The leathers presented tensile strength values between 1 and 3 MPa, the ones with BC showing significant higher strengths (p < 0.05), and the presence of probiotics significantly decreasing the tensile strength values. The shear force was also affected by the matrix (being higher for the BC-based leathers) and by the presence of L. acidophilus (which significantly decreased the shear force values). After the dehydration process, the viability of B. coagulans was reduced in about 1 log cycle when compared to that of the film-forming dispersion, showing about 7.6 log cfu g-1, while L. acidophilus did not show enough viability for counting (< 3 log cfu g-1). The viability of B. coagulans remained constant up to 187 days of storage at 25 °C. During the simulated passage through gastrointestinal tract, B. coagulans BC4 resisted well to the pH and enzimatic conditions of the stomach and duodenum, BC- and starch-based leathers released, respectively, 7.45 ± 0.12 e 7.77 ± 0.33 log cfu g-1 in the small intestine (without significant differences between matrices). Therefore, banana leathers can be promising non-dairy foods as carriers of B. coagulans BC4, however, L. acidophilus LA-3 could not be considered a suitable strain for the proposed process. It is recommended that a sensory analysis be carried out in the future with the different formulations of banana leathers to understand how differences in the texture can influence the product acceptance. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-11-08T15:04:43Z |
dc.date.available.fl_str_mv |
2021-11-08T15:04:43Z |
dc.date.issued.fl_str_mv |
2021-08-18 |
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info:eu-repo/semantics/masterThesis |
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NIRO, Carolina Madazio. Fitas comestíveis de banana como veículos para probióticos. 2021. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15082. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/15082 |
identifier_str_mv |
NIRO, Carolina Madazio. Fitas comestíveis de banana como veículos para probióticos. 2021. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15082. |
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Universidade Federal de São Carlos Câmpus São Carlos |
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Universidade Federal de São Carlos Câmpus São Carlos |
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