Fitas comestíveis de banana como veículos para probióticos

Detalhes bibliográficos
Autor(a) principal: Niro, Carolina Madazio
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/15082
Resumo: Considering the growing demand for products that simultaneously meet the requirements of healthiness, good sensory acceptance and convenience, snacks based on fruits or vegetables have been a strong market trend. Another trend is that of food containing probiotics. However, most probiotic foods available on the market are dairy products. With a large number of people with restrictions to the consumption of dairy products and even to animal-based products in general, there is a growing need to introduce non-dairy probiotic foods to the market. The objective of this project was to produce a probiotic banana leather. The fruit leathers were made from banana puree and a film forming matrix. Two matrices were compared – a digestible one (starch) and a non-digestible one (bacterial cellulose - BC), and two probiotic strains (one sporogenic and one non-sporogenic - Bacillus coagulans BC4 and Lactobacillus acidophilus LA-3, respectively), in addition to the presence of a plasticizer and prebiotic agent (yacon syrup). The different formulations were evaluated in terms of their physical properties and viability of the strains after oven drying at 50 °C, during storage at 25 °C and along the simulated passage through the gastrointestinal tract. The leathers presented tensile strength values between 1 and 3 MPa, the ones with BC showing significant higher strengths (p < 0.05), and the presence of probiotics significantly decreasing the tensile strength values. The shear force was also affected by the matrix (being higher for the BC-based leathers) and by the presence of L. acidophilus (which significantly decreased the shear force values). After the dehydration process, the viability of B. coagulans was reduced in about 1 log cycle when compared to that of the film-forming dispersion, showing about 7.6 log cfu g-1, while L. acidophilus did not show enough viability for counting (< 3 log cfu g-1). The viability of B. coagulans remained constant up to 187 days of storage at 25 °C. During the simulated passage through gastrointestinal tract, B. coagulans BC4 resisted well to the pH and enzimatic conditions of the stomach and duodenum, BC- and starch-based leathers released, respectively, 7.45 ± 0.12 e 7.77 ± 0.33 log cfu g-1 in the small intestine (without significant differences between matrices). Therefore, banana leathers can be promising non-dairy foods as carriers of B. coagulans BC4, however, L. acidophilus LA-3 could not be considered a suitable strain for the proposed process. It is recommended that a sensory analysis be carried out in the future with the different formulations of banana leathers to understand how differences in the texture can influence the product acceptance.
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spelling Niro, Carolina MadazioAzeredo, Henriette Monteiro Cordeiro dehttp://lattes.cnpq.br/8516223928904348http://lattes.cnpq.br/494649594516416930a1acc4-a481-4f0d-8fed-119c8fcd45d52021-11-08T15:04:43Z2021-11-08T15:04:43Z2021-08-18NIRO, Carolina Madazio. Fitas comestíveis de banana como veículos para probióticos. 2021. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15082.https://repositorio.ufscar.br/handle/ufscar/15082Considering the growing demand for products that simultaneously meet the requirements of healthiness, good sensory acceptance and convenience, snacks based on fruits or vegetables have been a strong market trend. Another trend is that of food containing probiotics. However, most probiotic foods available on the market are dairy products. With a large number of people with restrictions to the consumption of dairy products and even to animal-based products in general, there is a growing need to introduce non-dairy probiotic foods to the market. The objective of this project was to produce a probiotic banana leather. The fruit leathers were made from banana puree and a film forming matrix. Two matrices were compared – a digestible one (starch) and a non-digestible one (bacterial cellulose - BC), and two probiotic strains (one sporogenic and one non-sporogenic - Bacillus coagulans BC4 and Lactobacillus acidophilus LA-3, respectively), in addition to the presence of a plasticizer and prebiotic agent (yacon syrup). The different formulations were evaluated in terms of their physical properties and viability of the strains after oven drying at 50 °C, during storage at 25 °C and along the simulated passage through the gastrointestinal tract. The leathers presented tensile strength values between 1 and 3 MPa, the ones with BC showing significant higher strengths (p < 0.05), and the presence of probiotics significantly decreasing the tensile strength values. The shear force was also affected by the matrix (being higher for the BC-based leathers) and by the presence of L. acidophilus (which significantly decreased the shear force values). After the dehydration process, the viability of B. coagulans was reduced in about 1 log cycle when compared to that of the film-forming dispersion, showing about 7.6 log cfu g-1, while L. acidophilus did not show enough viability for counting (< 3 log cfu g-1). The viability of B. coagulans remained constant up to 187 days of storage at 25 °C. During the simulated passage through gastrointestinal tract, B. coagulans BC4 resisted well to the pH and enzimatic conditions of the stomach and duodenum, BC- and starch-based leathers released, respectively, 7.45 ± 0.12 e 7.77 ± 0.33 log cfu g-1 in the small intestine (without significant differences between matrices). Therefore, banana leathers can be promising non-dairy foods as carriers of B. coagulans BC4, however, L. acidophilus LA-3 could not be considered a suitable strain for the proposed process. It is recommended that a sensory analysis be carried out in the future with the different formulations of banana leathers to understand how differences in the texture can influence the product acceptance.Considerando a grande demanda por produtos que atendam simultaneamente aos requisitos de saudabilidade, boa aceitação sensorial e conveniência, snacks à base de frutas ou hortaliças têm se apresentado como uma forte tendência no mercado. Outra tendência são alimentos contendo probióticos. Porém, a grande maioria de alimentos probióticos disponíveis no mercado atualmente são lácteos. Com uma grande quantidade de pessoas com restrições ao consumo de laticínios e mesmo de produtos de origem animal no geral, cresce a necessidade de introduzir no mercado alimentos probióticos não-lácteos. O objetivo do trabalho foi desenvolver uma fita de banana probiótica. As fitas foram formuladas a partir de polpa de banana e uma matriz filmogênica. Foram testadas duas matrizes - uma digerível (amido) e uma não-digerível (celulose bacteriana - CB) - e duas cepas probióticas (esporogênica e não-esporogênica – Bacillus coagulans BC4 e Lactobacillus acidophilus LA-3, respectivamente), além da presença de um agente plastificante (xarope de yacon). As diferentes formulações foram avaliadas em termos de suas propriedades físicas e viabilidade das cepas após o processo de secagem em estufa a 50 °C, durante estocagem a 25 °C e ao longo da passagem simulada pelo trato gastrointestinal. As fitas apresentaram valores de resistência à tração entre 1 e 3 MPa, as fitas feitas com CB apresentaram resistência significativamente (p < 0,05) maior que as fitas feitas com amido, e a presença de probióticos diminuiu significativamente a resistência à tração. A força de cisalhamento também foi afetada pela matriz (sendo maior para as fitas de CB) e pela presença de L. acidophilus (que diminuiu a força de cisalhamento). Após a secagem, a viabilidade de B. coagulans reduziu em cerca de 1 ciclo log em relação à dispersão filmogênica, apresentando cerca de 7,6 log UFC g-1, enquanto L. acidophilus não apresentou viabilidade suficiente para contagem (< 3 log UFC g-1). A viabilidade de B. coagulans permaneceu constante até 187 dias de estocagem em 25 °C. Durante a passagem simulada pelo trato gastrointestinal, as fitas contendo B. coagulans BC4 foram resistentes às condições de pH e enzimáticas do estômago e duodeno, liberando 7,45 ± 0,12 e 7,77 ± 0,33 log UFC g-1 no intestino para as fitas à base de CB e amido, respectivamente (sem diferença significativa para as matrizes). Portanto, fitas de banana podem ser alimentos não-lácteos promissores como carreadores de B. coagulans BC4, porém, L. acidophilus LA-3 não poderia ser considerada uma cepa adequada para o processo proposto. Indica-se que seja feita futuramente uma análise sensorial com as diferentes formulações de fitas de banana para entender como as diferenças na textura das fitas podem influenciar a aceitação do produto.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES: 88882.426523/2019-01porUniversidade Federal de São CarlosCâmpus São CarlosPrograma de Pós-Graduação em Biotecnologia - PPGBiotecUFSCarAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessFitas de frutasProbióticosPolissacarídeosCelulose bacterianaSnacks saudáveisFruit leathersProbioticsPolysaccharidesBacterial celluloseHealthy snacksCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS::MICROBIOLOGIA DE ALIMENTOSCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOSCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOSFitas comestíveis de banana como veículos para probióticosBanana leathers as probiotic carriersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis6006000bdfe530-ac00-4c9a-9a2f-a06e4dd13055reponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINALDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdfDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdfDissertação de mestradoapplication/pdf2304592https://repositorio.ufscar.br/bitstream/ufscar/15082/1/Disserta%c3%a7%c3%a3o%20de%20Mestrado%20-%20PPGBiotec%20-%20Carolina%20Madazio%20Niro%20-%20Vers%c3%a3o%20Final.pdfc9f2ee32734abf1d0735465df705881fMD51Carta Comprovante.pdfCarta Comprovante.pdfCarta Comprovanteapplication/pdf694988https://repositorio.ufscar.br/bitstream/ufscar/15082/2/Carta%20Comprovante.pdff1a8ec82738c7f5c9e4113b1661c9a34MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufscar.br/bitstream/ufscar/15082/3/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD53TEXTDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdf.txtDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdf.txtExtracted texttext/plain155893https://repositorio.ufscar.br/bitstream/ufscar/15082/4/Disserta%c3%a7%c3%a3o%20de%20Mestrado%20-%20PPGBiotec%20-%20Carolina%20Madazio%20Niro%20-%20Vers%c3%a3o%20Final.pdf.txt6cf5c85f7aee56ddf4c74e62942c2fa7MD54Carta Comprovante.pdf.txtCarta Comprovante.pdf.txtExtracted texttext/plain1198https://repositorio.ufscar.br/bitstream/ufscar/15082/6/Carta%20Comprovante.pdf.txt06db3f35d9e8528ef8b564cfd7debea0MD56THUMBNAILDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdf.jpgDissertação de Mestrado - PPGBiotec - Carolina Madazio Niro - Versão Final.pdf.jpgIM Thumbnailimage/jpeg10042https://repositorio.ufscar.br/bitstream/ufscar/15082/5/Disserta%c3%a7%c3%a3o%20de%20Mestrado%20-%20PPGBiotec%20-%20Carolina%20Madazio%20Niro%20-%20Vers%c3%a3o%20Final.pdf.jpgb1dc9f9f20af3c497e654ceb14e88b5eMD55Carta Comprovante.pdf.jpgCarta Comprovante.pdf.jpgIM Thumbnailimage/jpeg6319https://repositorio.ufscar.br/bitstream/ufscar/15082/7/Carta%20Comprovante.pdf.jpg670bf13d485e18d126ea2aefb464ce50MD57ufscar/150822023-09-18 18:32:19.95oai:repositorio.ufscar.br:ufscar/15082Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:32:19Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Fitas comestíveis de banana como veículos para probióticos
dc.title.alternative.eng.fl_str_mv Banana leathers as probiotic carriers
title Fitas comestíveis de banana como veículos para probióticos
spellingShingle Fitas comestíveis de banana como veículos para probióticos
Niro, Carolina Madazio
Fitas de frutas
Probióticos
Polissacarídeos
Celulose bacteriana
Snacks saudáveis
Fruit leathers
Probiotics
Polysaccharides
Bacterial cellulose
Healthy snacks
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS::MICROBIOLOGIA DE ALIMENTOS
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS
title_short Fitas comestíveis de banana como veículos para probióticos
title_full Fitas comestíveis de banana como veículos para probióticos
title_fullStr Fitas comestíveis de banana como veículos para probióticos
title_full_unstemmed Fitas comestíveis de banana como veículos para probióticos
title_sort Fitas comestíveis de banana como veículos para probióticos
author Niro, Carolina Madazio
author_facet Niro, Carolina Madazio
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/4946495945164169
dc.contributor.author.fl_str_mv Niro, Carolina Madazio
dc.contributor.advisor1.fl_str_mv Azeredo, Henriette Monteiro Cordeiro de
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8516223928904348
dc.contributor.authorID.fl_str_mv 30a1acc4-a481-4f0d-8fed-119c8fcd45d5
contributor_str_mv Azeredo, Henriette Monteiro Cordeiro de
dc.subject.por.fl_str_mv Fitas de frutas
Probióticos
Polissacarídeos
Celulose bacteriana
Snacks saudáveis
topic Fitas de frutas
Probióticos
Polissacarídeos
Celulose bacteriana
Snacks saudáveis
Fruit leathers
Probiotics
Polysaccharides
Bacterial cellulose
Healthy snacks
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS::MICROBIOLOGIA DE ALIMENTOS
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS
dc.subject.eng.fl_str_mv Fruit leathers
Probiotics
Polysaccharides
Bacterial cellulose
Healthy snacks
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS::MICROBIOLOGIA DE ALIMENTOS
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::TECNOLOGIA DE ALIMENTOS
description Considering the growing demand for products that simultaneously meet the requirements of healthiness, good sensory acceptance and convenience, snacks based on fruits or vegetables have been a strong market trend. Another trend is that of food containing probiotics. However, most probiotic foods available on the market are dairy products. With a large number of people with restrictions to the consumption of dairy products and even to animal-based products in general, there is a growing need to introduce non-dairy probiotic foods to the market. The objective of this project was to produce a probiotic banana leather. The fruit leathers were made from banana puree and a film forming matrix. Two matrices were compared – a digestible one (starch) and a non-digestible one (bacterial cellulose - BC), and two probiotic strains (one sporogenic and one non-sporogenic - Bacillus coagulans BC4 and Lactobacillus acidophilus LA-3, respectively), in addition to the presence of a plasticizer and prebiotic agent (yacon syrup). The different formulations were evaluated in terms of their physical properties and viability of the strains after oven drying at 50 °C, during storage at 25 °C and along the simulated passage through the gastrointestinal tract. The leathers presented tensile strength values between 1 and 3 MPa, the ones with BC showing significant higher strengths (p < 0.05), and the presence of probiotics significantly decreasing the tensile strength values. The shear force was also affected by the matrix (being higher for the BC-based leathers) and by the presence of L. acidophilus (which significantly decreased the shear force values). After the dehydration process, the viability of B. coagulans was reduced in about 1 log cycle when compared to that of the film-forming dispersion, showing about 7.6 log cfu g-1, while L. acidophilus did not show enough viability for counting (< 3 log cfu g-1). The viability of B. coagulans remained constant up to 187 days of storage at 25 °C. During the simulated passage through gastrointestinal tract, B. coagulans BC4 resisted well to the pH and enzimatic conditions of the stomach and duodenum, BC- and starch-based leathers released, respectively, 7.45 ± 0.12 e 7.77 ± 0.33 log cfu g-1 in the small intestine (without significant differences between matrices). Therefore, banana leathers can be promising non-dairy foods as carriers of B. coagulans BC4, however, L. acidophilus LA-3 could not be considered a suitable strain for the proposed process. It is recommended that a sensory analysis be carried out in the future with the different formulations of banana leathers to understand how differences in the texture can influence the product acceptance.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-08T15:04:43Z
dc.date.available.fl_str_mv 2021-11-08T15:04:43Z
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dc.identifier.citation.fl_str_mv NIRO, Carolina Madazio. Fitas comestíveis de banana como veículos para probióticos. 2021. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15082.
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identifier_str_mv NIRO, Carolina Madazio. Fitas comestíveis de banana como veículos para probióticos. 2021. Dissertação (Mestrado em Biotecnologia) – Universidade Federal de São Carlos, São Carlos, 2021. Disponível em: https://repositorio.ufscar.br/handle/ufscar/15082.
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