Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFSCAR |
Texto Completo: | https://repositorio.ufscar.br/handle/ufscar/1645 |
Resumo: | Aluminium is very toxic to fish. Biomarkers have been using to indicate water contamination, however, there are few studies concerning tropical fish species. So, the neotropical fish Prochilodus lineatus, were exposed to aproximately 200 μg.L-1 of dissolved Al em acid pH (Al group), only to acid water (pH group) or to water with pH 7.0 (CTR group) for 6, 24, 96h and 10 days to evaluate its effects on haematological, osmo-ionic, metabolic and endocrine parameters besides morphological analysis by light and electronic microscopy. Fishes exposed to Al have all haematological and metabolic parameters increased when compared to CTR group. Osmo-ionic parameters were significantly smaller in fishes exposed to Al as much as the Na+/K+-ATPase enzyme and the CC number smaller Light microscopy reveal that fish exposed to Al in all experimental periods showed lesions widely distributed in the tissue and considered more severe when compared to fish from other groups. In the Al group, the main alterations found were hyperplasia of the epithelial cells, lamellar fusion and hypertrophy of mucous cells. In the animals of pH group, the main alteration was hipertrophy of epithelial cells, especially of the CC. Scanning electron microscopy allowed to see great disorganization in the lamellae of the fishes exposed to Al, detachment of branchial epithelium and reduction of the interlamellar space. Chloride cells with sponge aspect were noted in the pH and Al groups, besides alteration in the microcript of the pv in both groups. Transmission electron microscopy showed that animals from Al group have more CC displaced to the tip of the lamellae and that these CC have a less dense cytoplasm, with a smaller quantity of tubules and mitochondrias and nuclei with altered shapes. The thickness of the lamellae is bigger because of the swollen pv and the interlamellar spaces are reduced. There are, also, more vacuoles in the cytoplasm of these CC. Results allowed to conclude that exposure to Al in acid medium impairs mostly the hyperegulation capacity of the fish, since it affects the active uptake of ions in the gills and also caused serious structural alterations in the organ, leading to functional problems, jeopardizing the survival of the fishes in such situation. |
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Camargo, Marina Mori Pires deFernandes, Marisa Narcisohttp://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4783604Y6http://lattes.cnpq.br/3442998290367091736fe403-c818-462a-b925-f543257899842016-06-02T19:29:17Z2009-11-122016-06-02T19:29:17Z2009-06-05CAMARGO, Marina Mori Pires de. Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus. 2009. 109 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009.https://repositorio.ufscar.br/handle/ufscar/1645Aluminium is very toxic to fish. Biomarkers have been using to indicate water contamination, however, there are few studies concerning tropical fish species. So, the neotropical fish Prochilodus lineatus, were exposed to aproximately 200 μg.L-1 of dissolved Al em acid pH (Al group), only to acid water (pH group) or to water with pH 7.0 (CTR group) for 6, 24, 96h and 10 days to evaluate its effects on haematological, osmo-ionic, metabolic and endocrine parameters besides morphological analysis by light and electronic microscopy. Fishes exposed to Al have all haematological and metabolic parameters increased when compared to CTR group. Osmo-ionic parameters were significantly smaller in fishes exposed to Al as much as the Na+/K+-ATPase enzyme and the CC number smaller Light microscopy reveal that fish exposed to Al in all experimental periods showed lesions widely distributed in the tissue and considered more severe when compared to fish from other groups. In the Al group, the main alterations found were hyperplasia of the epithelial cells, lamellar fusion and hypertrophy of mucous cells. In the animals of pH group, the main alteration was hipertrophy of epithelial cells, especially of the CC. Scanning electron microscopy allowed to see great disorganization in the lamellae of the fishes exposed to Al, detachment of branchial epithelium and reduction of the interlamellar space. Chloride cells with sponge aspect were noted in the pH and Al groups, besides alteration in the microcript of the pv in both groups. Transmission electron microscopy showed that animals from Al group have more CC displaced to the tip of the lamellae and that these CC have a less dense cytoplasm, with a smaller quantity of tubules and mitochondrias and nuclei with altered shapes. The thickness of the lamellae is bigger because of the swollen pv and the interlamellar spaces are reduced. There are, also, more vacuoles in the cytoplasm of these CC. Results allowed to conclude that exposure to Al in acid medium impairs mostly the hyperegulation capacity of the fish, since it affects the active uptake of ions in the gills and also caused serious structural alterations in the organ, leading to functional problems, jeopardizing the survival of the fishes in such situation.O alumínio é muito tóxico para os peixes. Biomarcadores tem sido usados para indicar essa contaminação, entretanto são poucos os trabalhos com espécies tropicais. Assim, juvenis do peixe neotropical Prochilodus lineatus foram expostos a aproximadamente 200 μg.L-1 de Al dissolvido em pH ácido (grupo Al), somente pH ácido (grupo pH) ou à água com pH 7,0 (grupo CTR) por 6, 24, 96h e 10 dias para avaliar seus efeitos em parâmetros hematológicos, osmo-iônicos, metabólicos e endócrino, além dos parâmetros morfológicos, por meio de MO, MEV, MET, AFCC e EDS das brânquias. Os peixes expostos ao Al tiveram os parâmetros hematológicos e metabólicos aumentados em relação ao grupo CTR. Os parâmetros osmo-iônicos foram significativamente menores nesses peixes assim como a enzima Na+/K+-ATPase e o número de CC nos mesmos animais. A MO revelou que os peixes expostos ao Al mostraram lesões mais amplamente distribuídas nas brânquias e consideradas mais severas, quando comparados aos demais grupos, sendo que as principais alterações foram hiperplasia do epitélio branquial, fusão lamelar e hipertrofia das células mucosas. Nos animais expostos ao pH a principal alteração foi a hipertrofia celular, principalmente das CC. A MEV permitiu destacar o extenso desarranjo nas lamelas dos peixes dos grupos Al, descamação do epitélio além da redução do espaço interlamelar e fusão lamelar. CC com aspecto esponjoso foram notadas nos grupos pH e Al além da alteração no padrão das microcriptas das célula pv em ambos os grupos. Animais expostos ao Al mostraram ainda AFCC menores em relação aos CTR e o EDS indicou picos de Al maiores nesses grupos. A MET mostrou que os animais expostos ao Al têm mais CC deslocadas para as pontas das lamelas e que essas células apresentam o citoplasma menos denso, com uma menor quantidade de túbulos e mitocôndrias e núcleos formas alteradas, além de mais vacúolos. A espessura das lamelas é maior devido ao inchaço das pv, reduzindo o espaço interlamelar. Os resultados permitiram concluir que exposições ao Al em meio ácido prejudicam principalmente a capacidade de hiperregulação dos peixes afetando a tomada ativa de íons nas brânquias além de sérias alterações estruturais nas brânquias, comprometendo a sobrevivência dos animais nessas situações.Universidade Federal de Sao Carlosapplication/pdfporUniversidade Federal de São CarlosPrograma de Pós-Graduação em Ecologia e Recursos Naturais - PPGERNUFSCarBRPeixeAlumínioMarcadores biológicosBrânquiasPHAcidezProchilodus lineatusMorfologia branquialOsmorregulaçãoAlminiumAcidityBranchial morphologyOsmoregulationBiomarkersCIENCIAS BIOLOGICAS::ECOLOGIAExposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis-1-15470807a-1e63-4591-8511-0cb223b337f2info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL2471.pdfapplication/pdf5609066https://repositorio.ufscar.br/bitstream/ufscar/1645/1/2471.pdf5eb83354923bf2ef6bb644d15b85bbd2MD51THUMBNAIL2471.pdf.jpg2471.pdf.jpgIM Thumbnailimage/jpeg6591https://repositorio.ufscar.br/bitstream/ufscar/1645/2/2471.pdf.jpge1041b0b06d6d4399a064b3d9e2d204eMD52ufscar/16452023-09-18 18:30:44.417oai:repositorio.ufscar.br:ufscar/1645Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:30:44Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.por.fl_str_mv |
Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus |
title |
Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus |
spellingShingle |
Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus Camargo, Marina Mori Pires de Peixe Alumínio Marcadores biológicos Brânquias PH Acidez Prochilodus lineatus Morfologia branquial Osmorregulação Alminium Acidity Branchial morphology Osmoregulation Biomarkers CIENCIAS BIOLOGICAS::ECOLOGIA |
title_short |
Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus |
title_full |
Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus |
title_fullStr |
Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus |
title_full_unstemmed |
Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus |
title_sort |
Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus |
author |
Camargo, Marina Mori Pires de |
author_facet |
Camargo, Marina Mori Pires de |
author_role |
author |
dc.contributor.authorlattes.por.fl_str_mv |
http://lattes.cnpq.br/3442998290367091 |
dc.contributor.author.fl_str_mv |
Camargo, Marina Mori Pires de |
dc.contributor.advisor1.fl_str_mv |
Fernandes, Marisa Narciso |
dc.contributor.advisor1Lattes.fl_str_mv |
http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4783604Y6 |
dc.contributor.authorID.fl_str_mv |
736fe403-c818-462a-b925-f54325789984 |
contributor_str_mv |
Fernandes, Marisa Narciso |
dc.subject.por.fl_str_mv |
Peixe Alumínio Marcadores biológicos Brânquias PH Acidez Prochilodus lineatus Morfologia branquial Osmorregulação |
topic |
Peixe Alumínio Marcadores biológicos Brânquias PH Acidez Prochilodus lineatus Morfologia branquial Osmorregulação Alminium Acidity Branchial morphology Osmoregulation Biomarkers CIENCIAS BIOLOGICAS::ECOLOGIA |
dc.subject.eng.fl_str_mv |
Alminium Acidity Branchial morphology Osmoregulation Biomarkers |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::ECOLOGIA |
description |
Aluminium is very toxic to fish. Biomarkers have been using to indicate water contamination, however, there are few studies concerning tropical fish species. So, the neotropical fish Prochilodus lineatus, were exposed to aproximately 200 μg.L-1 of dissolved Al em acid pH (Al group), only to acid water (pH group) or to water with pH 7.0 (CTR group) for 6, 24, 96h and 10 days to evaluate its effects on haematological, osmo-ionic, metabolic and endocrine parameters besides morphological analysis by light and electronic microscopy. Fishes exposed to Al have all haematological and metabolic parameters increased when compared to CTR group. Osmo-ionic parameters were significantly smaller in fishes exposed to Al as much as the Na+/K+-ATPase enzyme and the CC number smaller Light microscopy reveal that fish exposed to Al in all experimental periods showed lesions widely distributed in the tissue and considered more severe when compared to fish from other groups. In the Al group, the main alterations found were hyperplasia of the epithelial cells, lamellar fusion and hypertrophy of mucous cells. In the animals of pH group, the main alteration was hipertrophy of epithelial cells, especially of the CC. Scanning electron microscopy allowed to see great disorganization in the lamellae of the fishes exposed to Al, detachment of branchial epithelium and reduction of the interlamellar space. Chloride cells with sponge aspect were noted in the pH and Al groups, besides alteration in the microcript of the pv in both groups. Transmission electron microscopy showed that animals from Al group have more CC displaced to the tip of the lamellae and that these CC have a less dense cytoplasm, with a smaller quantity of tubules and mitochondrias and nuclei with altered shapes. The thickness of the lamellae is bigger because of the swollen pv and the interlamellar spaces are reduced. There are, also, more vacuoles in the cytoplasm of these CC. Results allowed to conclude that exposure to Al in acid medium impairs mostly the hyperegulation capacity of the fish, since it affects the active uptake of ions in the gills and also caused serious structural alterations in the organ, leading to functional problems, jeopardizing the survival of the fishes in such situation. |
publishDate |
2009 |
dc.date.available.fl_str_mv |
2009-11-12 2016-06-02T19:29:17Z |
dc.date.issued.fl_str_mv |
2009-06-05 |
dc.date.accessioned.fl_str_mv |
2016-06-02T19:29:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CAMARGO, Marina Mori Pires de. Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus. 2009. 109 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufscar.br/handle/ufscar/1645 |
identifier_str_mv |
CAMARGO, Marina Mori Pires de. Exposições ao alumínio em meio ácido afetam as brânquias e a osmorregulação do peixe neotropical Prochilodus lineatus. 2009. 109 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009. |
url |
https://repositorio.ufscar.br/handle/ufscar/1645 |
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por |
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por |
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5470807a-1e63-4591-8511-0cb223b337f2 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de São Carlos |
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UFSCar |
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BR |
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Universidade Federal de São Carlos |
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