Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile

Detalhes bibliográficos
Autor(a) principal: Joshi,Chetna
Data de Publicação: 2012
Outros Autores: Khare,Sunil Kumar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003
Resumo: Regulation of xylanase production in two thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile was investigated. The expression of xylanase was found to be inducible in both the cases. Various carbon sources were tested so as to identify the inducers. Soy flour and oat spelt xylan induced maximum level of xylanase in Scytalidium thermophilum and Sporotrichum thermophile respectively. Induction of xylanase in Scytalidium thermophilum led to simultaneous induction of cellulase. The zymography of enzyme preparations revealed that different carbon sources caused differential expression of multiple isoforms of xylanase in Scytalidium thermophilum, but same isoforms were expressed by Sporotrichum thermophile irrespective of the carbon source used.
id TECPAR-1_0f4356789e018e2b074fa3c5578ed664
oai_identifier_str oai:scielo:S1516-89132012000100003
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophileInductionisoformsXylanasebiobleachingRegulation of xylanase production in two thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile was investigated. The expression of xylanase was found to be inducible in both the cases. Various carbon sources were tested so as to identify the inducers. Soy flour and oat spelt xylan induced maximum level of xylanase in Scytalidium thermophilum and Sporotrichum thermophile respectively. Induction of xylanase in Scytalidium thermophilum led to simultaneous induction of cellulase. The zymography of enzyme preparations revealed that different carbon sources caused differential expression of multiple isoforms of xylanase in Scytalidium thermophilum, but same isoforms were expressed by Sporotrichum thermophile irrespective of the carbon source used.Instituto de Tecnologia do Paraná - Tecpar2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003Brazilian Archives of Biology and Technology v.55 n.1 2012reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132012000100003info:eu-repo/semantics/openAccessJoshi,ChetnaKhare,Sunil Kumareng2012-03-29T00:00:00Zoai:scielo:S1516-89132012000100003Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2012-03-29T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
title Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
spellingShingle Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
Joshi,Chetna
Induction
isoforms
Xylanase
biobleaching
title_short Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
title_full Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
title_fullStr Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
title_full_unstemmed Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
title_sort Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile
author Joshi,Chetna
author_facet Joshi,Chetna
Khare,Sunil Kumar
author_role author
author2 Khare,Sunil Kumar
author2_role author
dc.contributor.author.fl_str_mv Joshi,Chetna
Khare,Sunil Kumar
dc.subject.por.fl_str_mv Induction
isoforms
Xylanase
biobleaching
topic Induction
isoforms
Xylanase
biobleaching
description Regulation of xylanase production in two thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile was investigated. The expression of xylanase was found to be inducible in both the cases. Various carbon sources were tested so as to identify the inducers. Soy flour and oat spelt xylan induced maximum level of xylanase in Scytalidium thermophilum and Sporotrichum thermophile respectively. Induction of xylanase in Scytalidium thermophilum led to simultaneous induction of cellulase. The zymography of enzyme preparations revealed that different carbon sources caused differential expression of multiple isoforms of xylanase in Scytalidium thermophilum, but same isoforms were expressed by Sporotrichum thermophile irrespective of the carbon source used.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132012000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.55 n.1 2012
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318274888859648

Registros relacionados