Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400636 |
Resumo: | The aim of this work was to optimize the growth conditions and continuous production of the enzyme using free and immobilized cells of inulinase by Penicillium funiculosum. The highest yield of enzyme (163.5U/mL) was obtained when the culture was incubated at 27oC and 200 rpm for 96h in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen source, respectively. When the cells of the P. funiculosum were immobilized on different carriers, especially linen fibers, their production ability was successfully maintained for seven successive batches. When the fermentation was carried out using inulin juice prepared from Jerusalem artichoke tubers (in place of pure inulin), inulinase production could be sustained till the second cultivation batch of the P. funiculosum immobilized on linen fibers, yielding 122 U/mL enzyme. Results proved the feasibility of using crude inulin juice as a simple and economic carbon source for the production of inulinase. |
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Brazilian Archives of Biology and Technology |
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Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36Penicillium funiculosumbiochemical inulinaseinulinrepeated batch cultivationThe aim of this work was to optimize the growth conditions and continuous production of the enzyme using free and immobilized cells of inulinase by Penicillium funiculosum. The highest yield of enzyme (163.5U/mL) was obtained when the culture was incubated at 27oC and 200 rpm for 96h in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen source, respectively. When the cells of the P. funiculosum were immobilized on different carriers, especially linen fibers, their production ability was successfully maintained for seven successive batches. When the fermentation was carried out using inulin juice prepared from Jerusalem artichoke tubers (in place of pure inulin), inulinase production could be sustained till the second cultivation batch of the P. funiculosum immobilized on linen fibers, yielding 122 U/mL enzyme. Results proved the feasibility of using crude inulin juice as a simple and economic carbon source for the production of inulinase.Instituto de Tecnologia do Paraná - Tecpar2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400636Brazilian Archives of Biology and Technology v.58 n.4 2015reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201500167info:eu-repo/semantics/openAccessDanial,Enas NabilAyaz,Najla Osman AlyAlnahdi,Hanan Saeed Omereng2015-10-26T00:00:00Zoai:scielo:S1516-89132015000400636Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-10-26T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36 |
title |
Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36 |
spellingShingle |
Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36 Danial,Enas Nabil Penicillium funiculosum biochemical inulinase inulin repeated batch cultivation |
title_short |
Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36 |
title_full |
Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36 |
title_fullStr |
Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36 |
title_full_unstemmed |
Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36 |
title_sort |
Production of Inulinase by Free and Immobilized Cells of Penicillium funiculosum p.36 |
author |
Danial,Enas Nabil |
author_facet |
Danial,Enas Nabil Ayaz,Najla Osman Aly Alnahdi,Hanan Saeed Omer |
author_role |
author |
author2 |
Ayaz,Najla Osman Aly Alnahdi,Hanan Saeed Omer |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Danial,Enas Nabil Ayaz,Najla Osman Aly Alnahdi,Hanan Saeed Omer |
dc.subject.por.fl_str_mv |
Penicillium funiculosum biochemical inulinase inulin repeated batch cultivation |
topic |
Penicillium funiculosum biochemical inulinase inulin repeated batch cultivation |
description |
The aim of this work was to optimize the growth conditions and continuous production of the enzyme using free and immobilized cells of inulinase by Penicillium funiculosum. The highest yield of enzyme (163.5U/mL) was obtained when the culture was incubated at 27oC and 200 rpm for 96h in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen source, respectively. When the cells of the P. funiculosum were immobilized on different carriers, especially linen fibers, their production ability was successfully maintained for seven successive batches. When the fermentation was carried out using inulin juice prepared from Jerusalem artichoke tubers (in place of pure inulin), inulinase production could be sustained till the second cultivation batch of the P. funiculosum immobilized on linen fibers, yielding 122 U/mL enzyme. Results proved the feasibility of using crude inulin juice as a simple and economic carbon source for the production of inulinase. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400636 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400636 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-8913201500167 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.58 n.4 2015 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276948262912 |