New isolate for enhancement production of microbial inulinase
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/28265 |
Resumo: | The optimization of growth conditions for the production of inulinase by Penicillium funiculosum cells were studied as well as the continuous production of the enzyme using immobilized cells. The highest amount of enzyme (163.5U/mL) was obtained when the producing cells were incubated for 96 hours at 27oC and 200 rpm in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen sources respectively. However, when the cells of the tested microorganism were adsorbed on different carriers, especially linen fibers, their production ability was also successfully maintained, to different extends, for seven successive batches. Moreover, commercially pure inulin is very expensive in only small quantities, this fermentation medium was later substituted by a crude inulin solution obtained from Jerusalem artichoke tubers (Helianthus tuberosus). The crude inulin juice was able to sustain inulinase production during the second batch cultivation of the P. funiculosum, immobilized by their adsorption on linen fibers, in a satisfactory level of about 122U/mL. Moreover, the use of the previously mentioned crude inulin preparation was also compared to the use of either complete or minimal media, composed solely of 1% pure inulin. The method, adopted in this study for inulinase production, is simple, economic, time saving, non-toxic to the microorganism and the loaded linen pads are reusable. |
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New isolate for enhancement production of microbial inulinase Penicillium funiculosumbiochemical inulinaseinulinContinuous productionAgricultural SciencesThe optimization of growth conditions for the production of inulinase by Penicillium funiculosum cells were studied as well as the continuous production of the enzyme using immobilized cells. The highest amount of enzyme (163.5U/mL) was obtained when the producing cells were incubated for 96 hours at 27oC and 200 rpm in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen sources respectively. However, when the cells of the tested microorganism were adsorbed on different carriers, especially linen fibers, their production ability was also successfully maintained, to different extends, for seven successive batches. Moreover, commercially pure inulin is very expensive in only small quantities, this fermentation medium was later substituted by a crude inulin solution obtained from Jerusalem artichoke tubers (Helianthus tuberosus). The crude inulin juice was able to sustain inulinase production during the second batch cultivation of the P. funiculosum, immobilized by their adsorption on linen fibers, in a satisfactory level of about 122U/mL. Moreover, the use of the previously mentioned crude inulin preparation was also compared to the use of either complete or minimal media, composed solely of 1% pure inulin. The method, adopted in this study for inulinase production, is simple, economic, time saving, non-toxic to the microorganism and the loaded linen pads are reusable.EDUFU2015-09-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/2826510.14393/BJ-v31n5a2015-28265Bioscience Journal ; Vol. 31 No. 5 (2015): Sept./Oct.; 1550-1560Bioscience Journal ; v. 31 n. 5 (2015): Sept./Oct.; 1550-15601981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/28265/17145Egypt; ContemporaryCopyright (c) 2015 Enas Nabil Danial, Nayera A. M. Abdelwahedhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDanial, Enas NabilAbdelwahed, Nayera A. M.2022-05-19T14:48:37Zoai:ojs.www.seer.ufu.br:article/28265Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-05-19T14:48:37Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
New isolate for enhancement production of microbial inulinase |
title |
New isolate for enhancement production of microbial inulinase |
spellingShingle |
New isolate for enhancement production of microbial inulinase Danial, Enas Nabil Penicillium funiculosum biochemical inulinase inulin Continuous production Agricultural Sciences |
title_short |
New isolate for enhancement production of microbial inulinase |
title_full |
New isolate for enhancement production of microbial inulinase |
title_fullStr |
New isolate for enhancement production of microbial inulinase |
title_full_unstemmed |
New isolate for enhancement production of microbial inulinase |
title_sort |
New isolate for enhancement production of microbial inulinase |
author |
Danial, Enas Nabil |
author_facet |
Danial, Enas Nabil Abdelwahed, Nayera A. M. |
author_role |
author |
author2 |
Abdelwahed, Nayera A. M. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Danial, Enas Nabil Abdelwahed, Nayera A. M. |
dc.subject.por.fl_str_mv |
Penicillium funiculosum biochemical inulinase inulin Continuous production Agricultural Sciences |
topic |
Penicillium funiculosum biochemical inulinase inulin Continuous production Agricultural Sciences |
description |
The optimization of growth conditions for the production of inulinase by Penicillium funiculosum cells were studied as well as the continuous production of the enzyme using immobilized cells. The highest amount of enzyme (163.5U/mL) was obtained when the producing cells were incubated for 96 hours at 27oC and 200 rpm in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen sources respectively. However, when the cells of the tested microorganism were adsorbed on different carriers, especially linen fibers, their production ability was also successfully maintained, to different extends, for seven successive batches. Moreover, commercially pure inulin is very expensive in only small quantities, this fermentation medium was later substituted by a crude inulin solution obtained from Jerusalem artichoke tubers (Helianthus tuberosus). The crude inulin juice was able to sustain inulinase production during the second batch cultivation of the P. funiculosum, immobilized by their adsorption on linen fibers, in a satisfactory level of about 122U/mL. Moreover, the use of the previously mentioned crude inulin preparation was also compared to the use of either complete or minimal media, composed solely of 1% pure inulin. The method, adopted in this study for inulinase production, is simple, economic, time saving, non-toxic to the microorganism and the loaded linen pads are reusable. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/28265 10.14393/BJ-v31n5a2015-28265 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/28265 |
identifier_str_mv |
10.14393/BJ-v31n5a2015-28265 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/28265/17145 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Enas Nabil Danial, Nayera A. M. Abdelwahed https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Enas Nabil Danial, Nayera A. M. Abdelwahed https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Egypt; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 31 No. 5 (2015): Sept./Oct.; 1550-1560 Bioscience Journal ; v. 31 n. 5 (2015): Sept./Oct.; 1550-1560 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069075394854912 |