Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m

Detalhes bibliográficos
Autor(a) principal: Terra,Danielle Amorim
Data de Publicação: 2007
Outros Autores: Amorim,Lúcia de Fátima, Catanho,Maria Tereza Jansen de Almeida, Fonseca,Adenilson de Souza da, Santos-Filho,Sebastião David, Brandão-Neto,José, Medeiros,Aldo da Cunha, Bernardo-Filho,Mario
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600015
Resumo: The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p<0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble fraction). The analysis of the results indicates that the extract could have substances that could interfere on the transport of stannous through the erythrocyte membrane alterating the labeling of blood cells with 99mTc.
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spelling Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99mArtemisia vulgaris L.Technetium-99mBlood constituentslabelingThe aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p<0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble fraction). The analysis of the results indicates that the extract could have substances that could interfere on the transport of stannous through the erythrocyte membrane alterating the labeling of blood cells with 99mTc.Instituto de Tecnologia do Paraná - Tecpar2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600015Brazilian Archives of Biology and Technology v.50 n.spe 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000600015info:eu-repo/semantics/openAccessTerra,Danielle AmorimAmorim,Lúcia de FátimaCatanho,Maria Tereza Jansen de AlmeidaFonseca,Adenilson de Souza daSantos-Filho,Sebastião DavidBrandão-Neto,JoséMedeiros,Aldo da CunhaBernardo-Filho,Marioeng2008-03-12T00:00:00Zoai:scielo:S1516-89132007000600015Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-03-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
title Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
spellingShingle Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
Terra,Danielle Amorim
Artemisia vulgaris L.
Technetium-99m
Blood constituents
labeling
title_short Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
title_full Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
title_fullStr Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
title_full_unstemmed Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
title_sort Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
author Terra,Danielle Amorim
author_facet Terra,Danielle Amorim
Amorim,Lúcia de Fátima
Catanho,Maria Tereza Jansen de Almeida
Fonseca,Adenilson de Souza da
Santos-Filho,Sebastião David
Brandão-Neto,José
Medeiros,Aldo da Cunha
Bernardo-Filho,Mario
author_role author
author2 Amorim,Lúcia de Fátima
Catanho,Maria Tereza Jansen de Almeida
Fonseca,Adenilson de Souza da
Santos-Filho,Sebastião David
Brandão-Neto,José
Medeiros,Aldo da Cunha
Bernardo-Filho,Mario
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Terra,Danielle Amorim
Amorim,Lúcia de Fátima
Catanho,Maria Tereza Jansen de Almeida
Fonseca,Adenilson de Souza da
Santos-Filho,Sebastião David
Brandão-Neto,José
Medeiros,Aldo da Cunha
Bernardo-Filho,Mario
dc.subject.por.fl_str_mv Artemisia vulgaris L.
Technetium-99m
Blood constituents
labeling
topic Artemisia vulgaris L.
Technetium-99m
Blood constituents
labeling
description The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p<0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble fraction). The analysis of the results indicates that the extract could have substances that could interfere on the transport of stannous through the erythrocyte membrane alterating the labeling of blood cells with 99mTc.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132007000600015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.50 n.spe 2007
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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