Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar

Detalhes bibliográficos
Autor(a) principal: Montañez Sáenz,Julio
Data de Publicação: 2000
Outros Autores: Téllez,Alejandro, de la Garza,Heliodoro, de la Luz Reyes,María, Contreras-Esquivel,Juan Carlos, Aguilar,Cristóbal Noé
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007
Resumo: Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.
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spelling Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivarExtractionpurificationpropertiespectinesterasepotatoPectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.Instituto de Tecnologia do Paraná - Tecpar2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007Brazilian Archives of Biology and Technology v.43 n.4 2000reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132000000400007info:eu-repo/semantics/openAccessMontañez Sáenz,JulioTéllez,Alejandrode la Garza,Heliodorode la Luz Reyes,MaríaContreras-Esquivel,Juan CarlosAguilar,Cristóbal Noéeng2007-03-12T00:00:00Zoai:scielo:S1516-89132000000400007Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-03-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
title Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
spellingShingle Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
Montañez Sáenz,Julio
Extraction
purification
properties
pectinesterase
potato
title_short Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
title_full Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
title_fullStr Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
title_full_unstemmed Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
title_sort Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
author Montañez Sáenz,Julio
author_facet Montañez Sáenz,Julio
Téllez,Alejandro
de la Garza,Heliodoro
de la Luz Reyes,María
Contreras-Esquivel,Juan Carlos
Aguilar,Cristóbal Noé
author_role author
author2 Téllez,Alejandro
de la Garza,Heliodoro
de la Luz Reyes,María
Contreras-Esquivel,Juan Carlos
Aguilar,Cristóbal Noé
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Montañez Sáenz,Julio
Téllez,Alejandro
de la Garza,Heliodoro
de la Luz Reyes,María
Contreras-Esquivel,Juan Carlos
Aguilar,Cristóbal Noé
dc.subject.por.fl_str_mv Extraction
purification
properties
pectinesterase
potato
topic Extraction
purification
properties
pectinesterase
potato
description Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132000000400007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.43 n.4 2000
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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