Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007 |
Resumo: | Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel. |
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Brazilian Archives of Biology and Technology |
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Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivarExtractionpurificationpropertiespectinesterasepotatoPectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel.Instituto de Tecnologia do Paraná - Tecpar2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007Brazilian Archives of Biology and Technology v.43 n.4 2000reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132000000400007info:eu-repo/semantics/openAccessMontañez Sáenz,JulioTéllez,Alejandrode la Garza,Heliodorode la Luz Reyes,MaríaContreras-Esquivel,Juan CarlosAguilar,Cristóbal Noéeng2007-03-12T00:00:00Zoai:scielo:S1516-89132000000400007Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-03-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar |
title |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar |
spellingShingle |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar Montañez Sáenz,Julio Extraction purification properties pectinesterase potato |
title_short |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar |
title_full |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar |
title_fullStr |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar |
title_full_unstemmed |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar |
title_sort |
Purification and some properties of pectinesterase from potato (Solanum tuberosum L.) alpha cultivar |
author |
Montañez Sáenz,Julio |
author_facet |
Montañez Sáenz,Julio Téllez,Alejandro de la Garza,Heliodoro de la Luz Reyes,María Contreras-Esquivel,Juan Carlos Aguilar,Cristóbal Noé |
author_role |
author |
author2 |
Téllez,Alejandro de la Garza,Heliodoro de la Luz Reyes,María Contreras-Esquivel,Juan Carlos Aguilar,Cristóbal Noé |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Montañez Sáenz,Julio Téllez,Alejandro de la Garza,Heliodoro de la Luz Reyes,María Contreras-Esquivel,Juan Carlos Aguilar,Cristóbal Noé |
dc.subject.por.fl_str_mv |
Extraction purification properties pectinesterase potato |
topic |
Extraction purification properties pectinesterase potato |
description |
Pectinesterase was extracted from potato alpha cultivar, purified and partially characterized The used protocol resulted in a 58.8-fold purification (51 850.2 units/mg protein) with 15.5% recovery of pectinesterase activity. The purified enzyme had a molecular weight of 27 kDa and its isoelectric point was around 4.5 with pH and temperature optima of 8.0 and 60°C, respectively. The purified enzyme had a single symmetric peak of specific activity after chromatographic steps. The homogeneity of the purified pectinesterase was confirmed by gel filtration and polyacrylamide electrophoresis gel. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132000000400007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.43 n.4 2000 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318268318482432 |