Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation

Detalhes bibliográficos
Autor(a) principal: Rahulan,Raji
Data de Publicação: 2014
Outros Autores: Manjunath,G, Nampoothiri,Kesavan Madhavan, Pandey,Ashok
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005
Resumo: A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP.
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spelling Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentationStreptomyces gedanensisMethionine amino peptidaseFermentationBox Behnken designA bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP.Instituto de Tecnologia do Paraná - Tecpar2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005Brazilian Archives of Biology and Technology v.57 n.2 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014000200005info:eu-repo/semantics/openAccessRahulan,RajiManjunath,GNampoothiri,Kesavan MadhavanPandey,Ashokeng2014-04-04T00:00:00Zoai:scielo:S1516-89132014000200005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-04-04T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
title Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
spellingShingle Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
Rahulan,Raji
Streptomyces gedanensis
Methionine amino peptidase
Fermentation
Box Behnken design
title_short Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
title_full Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
title_fullStr Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
title_full_unstemmed Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
title_sort Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
author Rahulan,Raji
author_facet Rahulan,Raji
Manjunath,G
Nampoothiri,Kesavan Madhavan
Pandey,Ashok
author_role author
author2 Manjunath,G
Nampoothiri,Kesavan Madhavan
Pandey,Ashok
author2_role author
author
author
dc.contributor.author.fl_str_mv Rahulan,Raji
Manjunath,G
Nampoothiri,Kesavan Madhavan
Pandey,Ashok
dc.subject.por.fl_str_mv Streptomyces gedanensis
Methionine amino peptidase
Fermentation
Box Behnken design
topic Streptomyces gedanensis
Methionine amino peptidase
Fermentation
Box Behnken design
description A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132014000200005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.57 n.2 2014
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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