Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005 |
Resumo: | A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP. |
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Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentationStreptomyces gedanensisMethionine amino peptidaseFermentationBox Behnken designA bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP.Instituto de Tecnologia do Paraná - Tecpar2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005Brazilian Archives of Biology and Technology v.57 n.2 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014000200005info:eu-repo/semantics/openAccessRahulan,RajiManjunath,GNampoothiri,Kesavan MadhavanPandey,Ashokeng2014-04-04T00:00:00Zoai:scielo:S1516-89132014000200005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-04-04T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation |
title |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation |
spellingShingle |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation Rahulan,Raji Streptomyces gedanensis Methionine amino peptidase Fermentation Box Behnken design |
title_short |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation |
title_full |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation |
title_fullStr |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation |
title_full_unstemmed |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation |
title_sort |
Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentation |
author |
Rahulan,Raji |
author_facet |
Rahulan,Raji Manjunath,G Nampoothiri,Kesavan Madhavan Pandey,Ashok |
author_role |
author |
author2 |
Manjunath,G Nampoothiri,Kesavan Madhavan Pandey,Ashok |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Rahulan,Raji Manjunath,G Nampoothiri,Kesavan Madhavan Pandey,Ashok |
dc.subject.por.fl_str_mv |
Streptomyces gedanensis Methionine amino peptidase Fermentation Box Behnken design |
topic |
Streptomyces gedanensis Methionine amino peptidase Fermentation Box Behnken design |
description |
A bioprocess was developed for extracellular MAP production from Streptomyces gedanensis by solid-state fermentation. Response surface methodology of Box Behken Design was performed to evaluate the interaction effects of most significant variables {inoculum size, (NH4)2SO4 concentration, MgSO4.7H2O and tryptone) on MAP production after the single parameter optimization and it resulted a maximum MAP production of 55.26 IU/g PUF after 120 h of fermentation. The concentrated crude MAP displayed a pH and temperature optimum of 8.5 and 50°C. By analyzing the thermal stability, the MAP was found to be stable in a temperature range of 50 to 55°C but lost about 50% of its activity at 65°C after 30 min. This is a first report of this kind of study for MAP. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132014000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.2 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276084236288 |