Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa

Detalhes bibliográficos
Autor(a) principal: Vasconcelos,André Belico de
Data de Publicação: 2009
Outros Autores: Souza,Patrícia Castanheira de, Varago,Fabiana Cristina, Lagares,Monique de Albuquerque, Santoro,Marcelo Matos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010
Resumo: The heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat production (microcalorimetry). Glucose and sperm cell concentrations were determined in order to measure the heat outputs resulting from sperm metabolism. Sperm viability, membrane integrity and sperm motility did not differ among the different glucose concentrations tested. Nevertheless, the highest heat output detected by the microcalorimeter was obtained with 6 mM glucose and 10(8) spermatozoa/mL. Since conduction microcalorimetry offered additional information on equine sperm metabolism, it could be used as a method to study equine semen preservation.
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spelling Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoaMicrocalorimetryGlucoseSpermatozoaSperm metabolismCell viabilityEquineThe heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat production (microcalorimetry). Glucose and sperm cell concentrations were determined in order to measure the heat outputs resulting from sperm metabolism. Sperm viability, membrane integrity and sperm motility did not differ among the different glucose concentrations tested. Nevertheless, the highest heat output detected by the microcalorimeter was obtained with 6 mM glucose and 10(8) spermatozoa/mL. Since conduction microcalorimetry offered additional information on equine sperm metabolism, it could be used as a method to study equine semen preservation.Instituto de Tecnologia do Paraná - Tecpar2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010Brazilian Archives of Biology and Technology v.52 n.5 2009reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132009000500010info:eu-repo/semantics/openAccessVasconcelos,André Belico deSouza,Patrícia Castanheira deVarago,Fabiana CristinaLagares,Monique de AlbuquerqueSantoro,Marcelo Matoseng2010-01-12T00:00:00Zoai:scielo:S1516-89132009000500010Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-01-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
title Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
spellingShingle Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
Vasconcelos,André Belico de
Microcalorimetry
Glucose
Spermatozoa
Sperm metabolism
Cell viability
Equine
title_short Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
title_full Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
title_fullStr Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
title_full_unstemmed Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
title_sort Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa
author Vasconcelos,André Belico de
author_facet Vasconcelos,André Belico de
Souza,Patrícia Castanheira de
Varago,Fabiana Cristina
Lagares,Monique de Albuquerque
Santoro,Marcelo Matos
author_role author
author2 Souza,Patrícia Castanheira de
Varago,Fabiana Cristina
Lagares,Monique de Albuquerque
Santoro,Marcelo Matos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vasconcelos,André Belico de
Souza,Patrícia Castanheira de
Varago,Fabiana Cristina
Lagares,Monique de Albuquerque
Santoro,Marcelo Matos
dc.subject.por.fl_str_mv Microcalorimetry
Glucose
Spermatozoa
Sperm metabolism
Cell viability
Equine
topic Microcalorimetry
Glucose
Spermatozoa
Sperm metabolism
Cell viability
Equine
description The heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat production (microcalorimetry). Glucose and sperm cell concentrations were determined in order to measure the heat outputs resulting from sperm metabolism. Sperm viability, membrane integrity and sperm motility did not differ among the different glucose concentrations tested. Nevertheless, the highest heat output detected by the microcalorimeter was obtained with 6 mM glucose and 10(8) spermatozoa/mL. Since conduction microcalorimetry offered additional information on equine sperm metabolism, it could be used as a method to study equine semen preservation.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132009000500010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.52 n.5 2009
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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