Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe

Detalhes bibliográficos
Autor(a) principal: Mello,Marcia O.
Data de Publicação: 2001
Outros Autores: Melo,Murilo, Appezzato-da-Glória,Beatriz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200014
Resumo: Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed on agar-solidified Murashige and Skoog medium without growth regulators at 25º C in the light. The establishment of these plantlets in soil was about 95%.
id TECPAR-1_4a0086a6dd79cde72efd15e75af99fde
oai_identifier_str oai:scielo:S1516-89132001000200014
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria RoscoeAdventitious budanatomymorphogenesisZingiberaceaeCallus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed on agar-solidified Murashige and Skoog medium without growth regulators at 25º C in the light. The establishment of these plantlets in soil was about 95%.Instituto de Tecnologia do Paraná - Tecpar2001-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200014Brazilian Archives of Biology and Technology v.44 n.2 2001reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132001000200014info:eu-repo/semantics/openAccessMello,Marcia O.Melo,MuriloAppezzato-da-Glória,Beatrizeng2001-11-07T00:00:00Zoai:scielo:S1516-89132001000200014Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2001-11-07T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
title Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
spellingShingle Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
Mello,Marcia O.
Adventitious bud
anatomy
morphogenesis
Zingiberaceae
title_short Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
title_full Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
title_fullStr Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
title_full_unstemmed Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
title_sort Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe
author Mello,Marcia O.
author_facet Mello,Marcia O.
Melo,Murilo
Appezzato-da-Glória,Beatriz
author_role author
author2 Melo,Murilo
Appezzato-da-Glória,Beatriz
author2_role author
author
dc.contributor.author.fl_str_mv Mello,Marcia O.
Melo,Murilo
Appezzato-da-Glória,Beatriz
dc.subject.por.fl_str_mv Adventitious bud
anatomy
morphogenesis
Zingiberaceae
topic Adventitious bud
anatomy
morphogenesis
Zingiberaceae
description Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed on agar-solidified Murashige and Skoog medium without growth regulators at 25º C in the light. The establishment of these plantlets in soil was about 95%.
publishDate 2001
dc.date.none.fl_str_mv 2001-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132001000200014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.44 n.2 2001
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318268666609664

Registros relacionados