In vitro organogenesis from root culture segments of Bixa orellana L. (Bixaceae)

• Autor(a) principal: Cruz, Ana Claudia Ferreira da
• Data de Publicação: 2014
• Outros Autores: Rocha, Diego Ismael, Iarema, Lourdes, Ventrella, Marília Contim, Otoni, Wagner Campos, Costa, Marcio Gilberto Cardoso, Paiva Neto, Vespasiano Borges
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1007/s11627-013-9580-2; http://www.locus.ufv.br/handle/123456789/23360
• topic: Annatto; Adventitious bud development; Cytokinins In vitro plant regeneration; Liquid cultures; Root explants

The organogenic potential of root explants derived from cultured seedlings of Bixa orellana L. (annatto) was investigated in response to different incubation conditions and either 4.44 μM 6-benzyladenine, 4.54 μM Thidiazuron, or 4.56 μM Zeatin. Explants cultured in liquid media with agitation generally showed better development of adventitious buds versus explants cultured on semi-solid media. The most adventitious buds developed from explants cultured in liquid media under a 16-h photoperiod. Use of Zeatin and Thidiazuron promoted the development of more adventitious buds than 6-benzyladenine but morphological abnormalities among regenerating shoots and plants were observed. Fewer adventitious buds developed from explants cultured in liquid media supplemented with 6-benzyladenine, but the buds gave rise to the highest percentage of morphologically normal regenerated plants. Histological analyses showed that adventitious buds originated from cell proliferation within the pericycle, opposite the protoxylem poles of the explant. Seedling root tissue is useful for in vitro propagation of B. orellana.