Direct RAPD evaluation of bacteria without conventional DNA extraction

Detalhes bibliográficos
Autor(a) principal: Araújo,Welington Luiz
Data de Publicação: 2004
Outros Autores: Angellis,Derlene Attili de, Azevedo,João Lúcio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006
Resumo: The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were centrifuged and the resulting preparation was suspended in TE buffer. After boiling, the cell suspension was diluted and 2.0 µl were used in reactions of 15 µl. The results showed no significant differences among the RAPD profile of the PCR reactions derived from the boiling and phenol extraction methods, suggesting the utilization of this method for genetic population analysis.
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spelling Direct RAPD evaluation of bacteria without conventional DNA extractionBacteriaboiling methodDNA extractionRAPDThe present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were centrifuged and the resulting preparation was suspended in TE buffer. After boiling, the cell suspension was diluted and 2.0 µl were used in reactions of 15 µl. The results showed no significant differences among the RAPD profile of the PCR reactions derived from the boiling and phenol extraction methods, suggesting the utilization of this method for genetic population analysis.Instituto de Tecnologia do Paraná - Tecpar2004-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006Brazilian Archives of Biology and Technology v.47 n.3 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000300006info:eu-repo/semantics/openAccessAraújo,Welington LuizAngellis,Derlene Attili deAzevedo,João Lúcioeng2004-08-03T00:00:00Zoai:scielo:S1516-89132004000300006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-08-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Direct RAPD evaluation of bacteria without conventional DNA extraction
title Direct RAPD evaluation of bacteria without conventional DNA extraction
spellingShingle Direct RAPD evaluation of bacteria without conventional DNA extraction
Araújo,Welington Luiz
Bacteria
boiling method
DNA extraction
RAPD
title_short Direct RAPD evaluation of bacteria without conventional DNA extraction
title_full Direct RAPD evaluation of bacteria without conventional DNA extraction
title_fullStr Direct RAPD evaluation of bacteria without conventional DNA extraction
title_full_unstemmed Direct RAPD evaluation of bacteria without conventional DNA extraction
title_sort Direct RAPD evaluation of bacteria without conventional DNA extraction
author Araújo,Welington Luiz
author_facet Araújo,Welington Luiz
Angellis,Derlene Attili de
Azevedo,João Lúcio
author_role author
author2 Angellis,Derlene Attili de
Azevedo,João Lúcio
author2_role author
author
dc.contributor.author.fl_str_mv Araújo,Welington Luiz
Angellis,Derlene Attili de
Azevedo,João Lúcio
dc.subject.por.fl_str_mv Bacteria
boiling method
DNA extraction
RAPD
topic Bacteria
boiling method
DNA extraction
RAPD
description The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were centrifuged and the resulting preparation was suspended in TE buffer. After boiling, the cell suspension was diluted and 2.0 µl were used in reactions of 15 µl. The results showed no significant differences among the RAPD profile of the PCR reactions derived from the boiling and phenol extraction methods, suggesting the utilization of this method for genetic population analysis.
publishDate 2004
dc.date.none.fl_str_mv 2004-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132004000300006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.47 n.3 2004
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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