The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5

Detalhes bibliográficos
Autor(a) principal: Lima,Francisco Esmaile de Sales
Data de Publicação: 2014
Outros Autores: Batista,Helena Beatriz Ruthner, Campos,Fabrício Souza, Kunert Filho,Hiran Castagnino, Cibulski,Samuel, Finoketti,Fernando, Arantes,Thalita, Spilki,Fernando, Roehe,Paulo Michel, Rijsewijk,Franciscus Antonius Maria, Franco,Ana Cláudia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007
Resumo: This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.
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spelling The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5BoHV-5V mRNAtransfectionThis study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.Instituto de Tecnologia do Paraná - Tecpar2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007Brazilian Archives of Biology and Technology v.57 n.1 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014000100007info:eu-repo/semantics/openAccessLima,Francisco Esmaile de SalesBatista,Helena Beatriz RuthnerCampos,Fabrício SouzaKunert Filho,Hiran CastagninoCibulski,SamuelFinoketti,FernandoArantes,ThalitaSpilki,FernandoRoehe,Paulo MichelRijsewijk,Franciscus Antonius MariaFranco,Ana Cláudiaeng2014-02-24T00:00:00Zoai:scielo:S1516-89132014000100007Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-02-24T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
title The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
spellingShingle The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
Lima,Francisco Esmaile de Sales
BoHV-5
V mRNA
transfection
title_short The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
title_full The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
title_fullStr The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
title_full_unstemmed The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
title_sort The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
author Lima,Francisco Esmaile de Sales
author_facet Lima,Francisco Esmaile de Sales
Batista,Helena Beatriz Ruthner
Campos,Fabrício Souza
Kunert Filho,Hiran Castagnino
Cibulski,Samuel
Finoketti,Fernando
Arantes,Thalita
Spilki,Fernando
Roehe,Paulo Michel
Rijsewijk,Franciscus Antonius Maria
Franco,Ana Cláudia
author_role author
author2 Batista,Helena Beatriz Ruthner
Campos,Fabrício Souza
Kunert Filho,Hiran Castagnino
Cibulski,Samuel
Finoketti,Fernando
Arantes,Thalita
Spilki,Fernando
Roehe,Paulo Michel
Rijsewijk,Franciscus Antonius Maria
Franco,Ana Cláudia
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima,Francisco Esmaile de Sales
Batista,Helena Beatriz Ruthner
Campos,Fabrício Souza
Kunert Filho,Hiran Castagnino
Cibulski,Samuel
Finoketti,Fernando
Arantes,Thalita
Spilki,Fernando
Roehe,Paulo Michel
Rijsewijk,Franciscus Antonius Maria
Franco,Ana Cláudia
dc.subject.por.fl_str_mv BoHV-5
V mRNA
transfection
topic BoHV-5
V mRNA
transfection
description This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132014000100007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.57 n.1 2014
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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