The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007 |
Resumo: | This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells. |
id |
TECPAR-1_6c451856dc8a5830303f8497c400ca88 |
---|---|
oai_identifier_str |
oai:scielo:S1516-89132014000100007 |
network_acronym_str |
TECPAR-1 |
network_name_str |
Brazilian Archives of Biology and Technology |
repository_id_str |
|
spelling |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5BoHV-5V mRNAtransfectionThis study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.Instituto de Tecnologia do Paraná - Tecpar2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007Brazilian Archives of Biology and Technology v.57 n.1 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014000100007info:eu-repo/semantics/openAccessLima,Francisco Esmaile de SalesBatista,Helena Beatriz RuthnerCampos,Fabrício SouzaKunert Filho,Hiran CastagninoCibulski,SamuelFinoketti,FernandoArantes,ThalitaSpilki,FernandoRoehe,Paulo MichelRijsewijk,Franciscus Antonius MariaFranco,Ana Cláudiaeng2014-02-24T00:00:00Zoai:scielo:S1516-89132014000100007Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-02-24T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5 |
title |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5 |
spellingShingle |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5 Lima,Francisco Esmaile de Sales BoHV-5 V mRNA transfection |
title_short |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5 |
title_full |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5 |
title_fullStr |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5 |
title_full_unstemmed |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5 |
title_sort |
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5 |
author |
Lima,Francisco Esmaile de Sales |
author_facet |
Lima,Francisco Esmaile de Sales Batista,Helena Beatriz Ruthner Campos,Fabrício Souza Kunert Filho,Hiran Castagnino Cibulski,Samuel Finoketti,Fernando Arantes,Thalita Spilki,Fernando Roehe,Paulo Michel Rijsewijk,Franciscus Antonius Maria Franco,Ana Cláudia |
author_role |
author |
author2 |
Batista,Helena Beatriz Ruthner Campos,Fabrício Souza Kunert Filho,Hiran Castagnino Cibulski,Samuel Finoketti,Fernando Arantes,Thalita Spilki,Fernando Roehe,Paulo Michel Rijsewijk,Franciscus Antonius Maria Franco,Ana Cláudia |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima,Francisco Esmaile de Sales Batista,Helena Beatriz Ruthner Campos,Fabrício Souza Kunert Filho,Hiran Castagnino Cibulski,Samuel Finoketti,Fernando Arantes,Thalita Spilki,Fernando Roehe,Paulo Michel Rijsewijk,Franciscus Antonius Maria Franco,Ana Cláudia |
dc.subject.por.fl_str_mv |
BoHV-5 V mRNA transfection |
topic |
BoHV-5 V mRNA transfection |
description |
This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132014000100007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.1 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276054876160 |