Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006 |
Resumo: | The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to identify the false negative results. |
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Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosisPolymerase Chain Reactiontuberculosisinternal controlnucleic acid amplificationuracyl DNA glycosilaseThe aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to identify the false negative results.Instituto de Tecnologia do Paraná - Tecpar2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006Brazilian Archives of Biology and Technology v.51 n.4 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000400006info:eu-repo/semantics/openAccessCortez-Herrera,ElizabethSperhacke,Rosa DeaBecker,DanielaKritski,AfrânioZaha,ArnaldoRossetti,Maria Lucia Rosaeng2008-08-26T00:00:00Zoai:scielo:S1516-89132008000400006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-08-26T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis |
title |
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis |
spellingShingle |
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis Cortez-Herrera,Elizabeth Polymerase Chain Reaction tuberculosis internal control nucleic acid amplification uracyl DNA glycosilase |
title_short |
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis |
title_full |
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis |
title_fullStr |
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis |
title_full_unstemmed |
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis |
title_sort |
Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis |
author |
Cortez-Herrera,Elizabeth |
author_facet |
Cortez-Herrera,Elizabeth Sperhacke,Rosa Dea Becker,Daniela Kritski,Afrânio Zaha,Arnaldo Rossetti,Maria Lucia Rosa |
author_role |
author |
author2 |
Sperhacke,Rosa Dea Becker,Daniela Kritski,Afrânio Zaha,Arnaldo Rossetti,Maria Lucia Rosa |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cortez-Herrera,Elizabeth Sperhacke,Rosa Dea Becker,Daniela Kritski,Afrânio Zaha,Arnaldo Rossetti,Maria Lucia Rosa |
dc.subject.por.fl_str_mv |
Polymerase Chain Reaction tuberculosis internal control nucleic acid amplification uracyl DNA glycosilase |
topic |
Polymerase Chain Reaction tuberculosis internal control nucleic acid amplification uracyl DNA glycosilase |
description |
The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to identify the false negative results. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132008000400006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.51 n.4 2008 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318272283148288 |