Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis

Detalhes bibliográficos
Autor(a) principal: Cortez-Herrera,Elizabeth
Data de Publicação: 2008
Outros Autores: Sperhacke,Rosa Dea, Becker,Daniela, Kritski,Afrânio, Zaha,Arnaldo, Rossetti,Maria Lucia Rosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006
Resumo: The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to identify the false negative results.
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spelling Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosisPolymerase Chain Reactiontuberculosisinternal controlnucleic acid amplificationuracyl DNA glycosilaseThe aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to identify the false negative results.Instituto de Tecnologia do Paraná - Tecpar2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006Brazilian Archives of Biology and Technology v.51 n.4 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000400006info:eu-repo/semantics/openAccessCortez-Herrera,ElizabethSperhacke,Rosa DeaBecker,DanielaKritski,AfrânioZaha,ArnaldoRossetti,Maria Lucia Rosaeng2008-08-26T00:00:00Zoai:scielo:S1516-89132008000400006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-08-26T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
title Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
spellingShingle Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
Cortez-Herrera,Elizabeth
Polymerase Chain Reaction
tuberculosis
internal control
nucleic acid amplification
uracyl DNA glycosilase
title_short Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
title_full Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
title_fullStr Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
title_full_unstemmed Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
title_sort Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis
author Cortez-Herrera,Elizabeth
author_facet Cortez-Herrera,Elizabeth
Sperhacke,Rosa Dea
Becker,Daniela
Kritski,Afrânio
Zaha,Arnaldo
Rossetti,Maria Lucia Rosa
author_role author
author2 Sperhacke,Rosa Dea
Becker,Daniela
Kritski,Afrânio
Zaha,Arnaldo
Rossetti,Maria Lucia Rosa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cortez-Herrera,Elizabeth
Sperhacke,Rosa Dea
Becker,Daniela
Kritski,Afrânio
Zaha,Arnaldo
Rossetti,Maria Lucia Rosa
dc.subject.por.fl_str_mv Polymerase Chain Reaction
tuberculosis
internal control
nucleic acid amplification
uracyl DNA glycosilase
topic Polymerase Chain Reaction
tuberculosis
internal control
nucleic acid amplification
uracyl DNA glycosilase
description The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to identify the false negative results.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000400006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.4 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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