Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020 |
Resumo: | Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged cultivations at 30 ºC. The highest level of α-amylase activity corresponded to 10.1 U. mL-1 and was obtained using sisal waste (2.7%) and urea (0.8%) in submerged fermentation after 3 days of cultivation. The partial characterization showed the best α-amylase activity at 50ºC and pH 7.0. These results are of great importance for the use of sisal waste as a substrate for biotechnological proposes. |
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Brazilian Archives of Biology and Technology |
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Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-productsα-amylasessugarcane bagassesisal wasteStreptomyces spApproximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged cultivations at 30 ºC. The highest level of α-amylase activity corresponded to 10.1 U. mL-1 and was obtained using sisal waste (2.7%) and urea (0.8%) in submerged fermentation after 3 days of cultivation. The partial characterization showed the best α-amylase activity at 50ºC and pH 7.0. These results are of great importance for the use of sisal waste as a substrate for biotechnological proposes.Instituto de Tecnologia do Paraná - Tecpar2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020Brazilian Archives of Biology and Technology v.55 n.5 2012reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132012000500020info:eu-repo/semantics/openAccessSantos,Édilla Ribeiro dosTeles,Zozilene Nascimento SantosCampos,Núria MarianaSouza,Diogo Angeli Jacinto deBispo,Aline Simões da RochaNascimento,Rodrigo Pires doeng2012-10-09T00:00:00Zoai:scielo:S1516-89132012000500020Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2012-10-09T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
title |
Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
spellingShingle |
Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products Santos,Édilla Ribeiro dos α-amylases sugarcane bagasse sisal waste Streptomyces sp |
title_short |
Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
title_full |
Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
title_fullStr |
Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
title_full_unstemmed |
Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
title_sort |
Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products |
author |
Santos,Édilla Ribeiro dos |
author_facet |
Santos,Édilla Ribeiro dos Teles,Zozilene Nascimento Santos Campos,Núria Mariana Souza,Diogo Angeli Jacinto de Bispo,Aline Simões da Rocha Nascimento,Rodrigo Pires do |
author_role |
author |
author2 |
Teles,Zozilene Nascimento Santos Campos,Núria Mariana Souza,Diogo Angeli Jacinto de Bispo,Aline Simões da Rocha Nascimento,Rodrigo Pires do |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Édilla Ribeiro dos Teles,Zozilene Nascimento Santos Campos,Núria Mariana Souza,Diogo Angeli Jacinto de Bispo,Aline Simões da Rocha Nascimento,Rodrigo Pires do |
dc.subject.por.fl_str_mv |
α-amylases sugarcane bagasse sisal waste Streptomyces sp |
topic |
α-amylases sugarcane bagasse sisal waste Streptomyces sp |
description |
Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged cultivations at 30 ºC. The highest level of α-amylase activity corresponded to 10.1 U. mL-1 and was obtained using sisal waste (2.7%) and urea (0.8%) in submerged fermentation after 3 days of cultivation. The partial characterization showed the best α-amylase activity at 50ºC and pH 7.0. These results are of great importance for the use of sisal waste as a substrate for biotechnological proposes. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132012000500020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.55 n.5 2012 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318275285221376 |