Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus

Detalhes bibliográficos
Autor(a) principal: Almeida,Andréa Farias de
Data de Publicação: 2010
Outros Autores: Macedo,Gorete Ribeiro de, Chan,Leslie Chee Loong, Pedrini,Márcia Regina da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200006
Resumo: In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL).
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spelling Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirusBaculovirusin vitro productionkinetic analysisSfMNPVIn this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL).Instituto de Tecnologia do Paraná - Tecpar2010-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200006Brazilian Archives of Biology and Technology v.53 n.2 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000200006info:eu-repo/semantics/openAccessAlmeida,Andréa Farias deMacedo,Gorete Ribeiro deChan,Leslie Chee LoongPedrini,Márcia Regina da Silvaeng2010-04-06T00:00:00Zoai:scielo:S1516-89132010000200006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-04-06T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
spellingShingle Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
Almeida,Andréa Farias de
Baculovirus
in vitro production
kinetic analysis
SfMNPV
title_short Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title_full Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title_fullStr Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title_full_unstemmed Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title_sort Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
author Almeida,Andréa Farias de
author_facet Almeida,Andréa Farias de
Macedo,Gorete Ribeiro de
Chan,Leslie Chee Loong
Pedrini,Márcia Regina da Silva
author_role author
author2 Macedo,Gorete Ribeiro de
Chan,Leslie Chee Loong
Pedrini,Márcia Regina da Silva
author2_role author
author
author
dc.contributor.author.fl_str_mv Almeida,Andréa Farias de
Macedo,Gorete Ribeiro de
Chan,Leslie Chee Loong
Pedrini,Márcia Regina da Silva
dc.subject.por.fl_str_mv Baculovirus
in vitro production
kinetic analysis
SfMNPV
topic Baculovirus
in vitro production
kinetic analysis
SfMNPV
description In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL).
publishDate 2010
dc.date.none.fl_str_mv 2010-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.2 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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