Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus

Detalhes bibliográficos
Autor(a) principal: Almeida, Andréa Farias
Data de Publicação: 2010
Outros Autores: Macedo, Gorete Ribeiro, Chan, Leslie Chee Loong, Pedrini, Márcia Regina da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/45091
Resumo: Neste trabalho, analisou-se o comportamento cinético das células Sf9 e Sf21 utilizadas na produção de biopesticida para o controle de Spodoptera frugiperda. Variáveis cinéticas, como velocidade específica máxima de crescimento, produtividade em células, velocidade média de infecção e a velocidade média de produção de OB foram determinadas durante a infecção destas linhagens com o vírus extracelular do nucleopoliedrovirus de S. frugiperda. A linhagem Sf9 resultou em melhores resultados de produção do baculovírus (5 x 10(8) OB/mL), quando comparada à linhagem Sf21 (2,5 x 10(8) OB/mL) e outras linhagens da literatura
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spelling Almeida, Andréa FariasMacedo, Gorete RibeiroChan, Leslie Chee LoongPedrini, Márcia Regina da Silva2021-11-30T13:57:48Z2021-11-30T13:57:48Z2010-04ALMEIDA, Andréa Farias de; MACEDO, Gorete Ribeiro de; CHAN, Leslie Chee Loong; PEDRINI, Márcia Regina da Silva. Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus. Brazilian Archives Of Biology And Technology, [S.L.], v. 53, n. 2, p. 285-291, abr. 2010. Disponível em: https://www.scielo.br/j/babt/a/mmtSMyKjpnfc4NNCgCLrrCx/?lang=en. Acesso em: 30 nov. 2021. http://dx.doi.org/10.1590/s1516-89132010000200006.1516-89131678-4324https://repositorio.ufrn.br/handle/123456789/4509110.1590/S1516-89132010000200006Neste trabalho, analisou-se o comportamento cinético das células Sf9 e Sf21 utilizadas na produção de biopesticida para o controle de Spodoptera frugiperda. Variáveis cinéticas, como velocidade específica máxima de crescimento, produtividade em células, velocidade média de infecção e a velocidade média de produção de OB foram determinadas durante a infecção destas linhagens com o vírus extracelular do nucleopoliedrovirus de S. frugiperda. A linhagem Sf9 resultou em melhores resultados de produção do baculovírus (5 x 10(8) OB/mL), quando comparada à linhagem Sf21 (2,5 x 10(8) OB/mL) e outras linhagens da literaturaIn this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL)ScieloAttribution-NonCommercial 3.0 Brazilhttp://creativecommons.org/licenses/by-nc/3.0/br/info:eu-repo/semantics/openAccessKinetic analysisSfMNPVBaculovirusIn vitro productionKinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8920https://repositorio.ufrn.br/bitstream/123456789/45091/2/license_rdf728dfda2fa81b274c619d08d1dfc1a03MD52ORIGINALKineticAnalysisInVitro_Pedrini_2010.pdfKineticAnalysisInVitro_Pedrini_2010.pdfapplication/pdf63987https://repositorio.ufrn.br/bitstream/123456789/45091/1/KineticAnalysisInVitro_Pedrini_2010.pdf9402690e4187004b19bd3614d5e54b2fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81569https://repositorio.ufrn.br/bitstream/123456789/45091/3/license.txt6e6f57145bc87daf99079f06b081ff9fMD53123456789/450912021-11-30 10:57:48.446oai:https://repositorio.ufrn.br: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ório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-30T13:57:48Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
spellingShingle Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
Almeida, Andréa Farias
Kinetic analysis
SfMNPV
Baculovirus
In vitro production
title_short Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title_full Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title_fullStr Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title_full_unstemmed Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
title_sort Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
author Almeida, Andréa Farias
author_facet Almeida, Andréa Farias
Macedo, Gorete Ribeiro
Chan, Leslie Chee Loong
Pedrini, Márcia Regina da Silva
author_role author
author2 Macedo, Gorete Ribeiro
Chan, Leslie Chee Loong
Pedrini, Márcia Regina da Silva
author2_role author
author
author
dc.contributor.author.fl_str_mv Almeida, Andréa Farias
Macedo, Gorete Ribeiro
Chan, Leslie Chee Loong
Pedrini, Márcia Regina da Silva
dc.subject.por.fl_str_mv Kinetic analysis
SfMNPV
Baculovirus
In vitro production
topic Kinetic analysis
SfMNPV
Baculovirus
In vitro production
description Neste trabalho, analisou-se o comportamento cinético das células Sf9 e Sf21 utilizadas na produção de biopesticida para o controle de Spodoptera frugiperda. Variáveis cinéticas, como velocidade específica máxima de crescimento, produtividade em células, velocidade média de infecção e a velocidade média de produção de OB foram determinadas durante a infecção destas linhagens com o vírus extracelular do nucleopoliedrovirus de S. frugiperda. A linhagem Sf9 resultou em melhores resultados de produção do baculovírus (5 x 10(8) OB/mL), quando comparada à linhagem Sf21 (2,5 x 10(8) OB/mL) e outras linhagens da literatura
publishDate 2010
dc.date.issued.fl_str_mv 2010-04
dc.date.accessioned.fl_str_mv 2021-11-30T13:57:48Z
dc.date.available.fl_str_mv 2021-11-30T13:57:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Andréa Farias de; MACEDO, Gorete Ribeiro de; CHAN, Leslie Chee Loong; PEDRINI, Márcia Regina da Silva. Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus. Brazilian Archives Of Biology And Technology, [S.L.], v. 53, n. 2, p. 285-291, abr. 2010. Disponível em: https://www.scielo.br/j/babt/a/mmtSMyKjpnfc4NNCgCLrrCx/?lang=en. Acesso em: 30 nov. 2021. http://dx.doi.org/10.1590/s1516-89132010000200006.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/45091
dc.identifier.issn.none.fl_str_mv 1516-8913
1678-4324
dc.identifier.doi.none.fl_str_mv 10.1590/S1516-89132010000200006
identifier_str_mv ALMEIDA, Andréa Farias de; MACEDO, Gorete Ribeiro de; CHAN, Leslie Chee Loong; PEDRINI, Márcia Regina da Silva. Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus. Brazilian Archives Of Biology And Technology, [S.L.], v. 53, n. 2, p. 285-291, abr. 2010. Disponível em: https://www.scielo.br/j/babt/a/mmtSMyKjpnfc4NNCgCLrrCx/?lang=en. Acesso em: 30 nov. 2021. http://dx.doi.org/10.1590/s1516-89132010000200006.
1516-8913
1678-4324
10.1590/S1516-89132010000200006
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http://creativecommons.org/licenses/by-nc/3.0/br/
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rights_invalid_str_mv Attribution-NonCommercial 3.0 Brazil
http://creativecommons.org/licenses/by-nc/3.0/br/
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