In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/25287 |
Resumo: | The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDS-Page of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV. |
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In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell linesInfectividade in vitro de Spodoptera frugiperda multiple nucleopolyhedrovirus a diferentes linhagens celulares de insetosBaculovirus; biological control; cell culture; fall armyworm; in vitro production; SfMNPVBaculovirus; controle biológico; cultura de células; lagarta-do-cartucho; produção in vitro; SfMNPVThe objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDS-Page of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.O objetivo deste trabalho foi avaliar uma gama de hospedeiros, in vitro, quanto à resposta a Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), um vírus patogênico à lagarta-do-cartucho (Spodoptera frugiperda; Lepidoptera: Noctuidae), para o posterior desenvolvimento de um biopesticida baseado em sistema de cultura de células. As linhagens celulares de Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286) e S. frugiperda (IPLB-SF-21AE e Sf9) foram testadas quanto a sua suscetibilidade a um isolado brasileiro de SfMNPV altamente virulento. Os efeitos citopáticos induzidos pelo vírus, a produção de partículas virais e a síntese de polipeptídeos virais foram examinados e comparados. Ambas as células de S. frugiperda apresentaram hipertrofia dos núcleos celulares e produção de muitos poliedros. O SDS-Page de proteínas com radiomarcação mostrou que as proteínas celulares apresentaram inibição de síntese, enquanto uma intensa banda de cerca de 30 kDa, reconhecida como poliedrina, era sintetizada. As outras linhagens celulares não apresentaram produção de poliedros, apesar de algumas terem apresentado alterações morfológicas e inibição de síntese proteica em resposta à infecção viral. As linhagens celulares SF-21 e Sf9 são recomendadas para a posterior produção in vitro de SfMNPV. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraEmpresa Brasileira de Pesquisa Agropecuária (Embrapa)Agricultural Innovation MarketplaceFundação de Apoio à Pesquisa do Distrito Federal (FAPDF)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sihler, WilliamSouza, Marlinda Lobo deValicente, Fernando HercosFalcão, RosanaSanches, Márcio Martinello2018-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/25287Pesquisa Agropecuaria Brasileira; v.53, n.1, jan. 2018; 1-9Pesquisa Agropecuária Brasileira; v.53, n.1, jan. 2018; 1-91678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/25287/14100https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17045https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17046https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17047https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17048https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17049https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17050Direitos autorais 2018 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2018-02-22T15:09:45Zoai:ojs.seer.sct.embrapa.br:article/25287Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2018-02-22T15:09:45Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines Infectividade in vitro de Spodoptera frugiperda multiple nucleopolyhedrovirus a diferentes linhagens celulares de insetos |
title |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines |
spellingShingle |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines Sihler, William Baculovirus; biological control; cell culture; fall armyworm; in vitro production; SfMNPV Baculovirus; controle biológico; cultura de células; lagarta-do-cartucho; produção in vitro; SfMNPV |
title_short |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines |
title_full |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines |
title_fullStr |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines |
title_full_unstemmed |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines |
title_sort |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines |
author |
Sihler, William |
author_facet |
Sihler, William Souza, Marlinda Lobo de Valicente, Fernando Hercos Falcão, Rosana Sanches, Márcio Martinello |
author_role |
author |
author2 |
Souza, Marlinda Lobo de Valicente, Fernando Hercos Falcão, Rosana Sanches, Márcio Martinello |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Agricultural Innovation Marketplace Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
dc.contributor.author.fl_str_mv |
Sihler, William Souza, Marlinda Lobo de Valicente, Fernando Hercos Falcão, Rosana Sanches, Márcio Martinello |
dc.subject.por.fl_str_mv |
Baculovirus; biological control; cell culture; fall armyworm; in vitro production; SfMNPV Baculovirus; controle biológico; cultura de células; lagarta-do-cartucho; produção in vitro; SfMNPV |
topic |
Baculovirus; biological control; cell culture; fall armyworm; in vitro production; SfMNPV Baculovirus; controle biológico; cultura de células; lagarta-do-cartucho; produção in vitro; SfMNPV |
description |
The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDS-Page of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02-16 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/25287 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/25287 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/25287/14100 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17045 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17046 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17047 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17048 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17049 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25287/17050 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2018 Pesquisa Agropecuária Brasileira info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2018 Pesquisa Agropecuária Brasileira |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.53, n.1, jan. 2018; 1-9 Pesquisa Agropecuária Brasileira; v.53, n.1, jan. 2018; 1-9 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416713629335552 |