Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005 |
Resumo: | Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20). |
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Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doublingGlycine maxFDAbinucleate pollen grainsmultinucleate pollen grainsspontaneous diploidizationAnthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).Instituto de Tecnologia do Paraná - Tecpar2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005Brazilian Archives of Biology and Technology v.47 n.5 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000500005info:eu-repo/semantics/openAccessCardoso,Milena BarcelosKaltchuk-Santos,ElianeMundstock,Elsa Cristina deBodanese-Zanettini,Maria Helenaeng2004-11-23T00:00:00Zoai:scielo:S1516-89132004000500005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-11-23T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling |
title |
Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling |
spellingShingle |
Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling Cardoso,Milena Barcelos Glycine max FDA binucleate pollen grains multinucleate pollen grains spontaneous diploidization |
title_short |
Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling |
title_full |
Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling |
title_fullStr |
Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling |
title_full_unstemmed |
Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling |
title_sort |
Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling |
author |
Cardoso,Milena Barcelos |
author_facet |
Cardoso,Milena Barcelos Kaltchuk-Santos,Eliane Mundstock,Elsa Cristina de Bodanese-Zanettini,Maria Helena |
author_role |
author |
author2 |
Kaltchuk-Santos,Eliane Mundstock,Elsa Cristina de Bodanese-Zanettini,Maria Helena |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cardoso,Milena Barcelos Kaltchuk-Santos,Eliane Mundstock,Elsa Cristina de Bodanese-Zanettini,Maria Helena |
dc.subject.por.fl_str_mv |
Glycine max FDA binucleate pollen grains multinucleate pollen grains spontaneous diploidization |
topic |
Glycine max FDA binucleate pollen grains multinucleate pollen grains spontaneous diploidization |
description |
Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20). |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132004000500005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.47 n.5 2004 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318269897637888 |